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Microbial degradation of textile effluent and in genotoxic effect on Allium cepa

Author Affiliations

  • 1Department of Biotechnology, National College, Tiruchirappalli-620 001, Tamil Nadu, India
  • 2Department of Biotechnology, National College, Tiruchirappalli-620 001, Tamil Nadu, India
  • 3Department of Biotechnology, National College, Tiruchirappalli-620 001, Tamil Nadu, India
  • 4Department of Biotechnology, National College, Tiruchirappalli-620 001, Tamil Nadu, India

Int. Res. J. Environment Sci., Volume 7, Issue (8), Pages 1-7, August,22 (2018)


Textile effluent discharged from the small scale industries was assessed. There was a total difference in the physico chemical characteristics of effluent sample and bacterial and fungal consortia degraded samples. The untreated effluent samples were highly colored, fishy odour, slight alkaline. The other physic chemical properties like total solids (TS) total suspended solids (TSS), total dissolved solids (TDS), biological oxygen demand (BOD), chemical oxygen demand (COD), dissolved oxygen (DO), hardness as CaCO3 alkalinity, bicarbonate (HCO3-) alkalinity, chloride, calcium, magnesium, sodium, sulphate, zinc, chromium, copper and lead were found to be above to the permissible level of WHO standards, which ensure the presence of pollutants in the textile effluent. From the polluted sites, four bacterial strains which are identified as indicator bacterial strains able to degrade the effluent and dye were used for the decolourization and degradation in vitro experiments. They are Bacillus subtilis (NCBT 012), Clostridium butyricum (NCBT 017), Enterobacter aerogens (NCBT 024) and Pseudomonas fluorescens (NCBT 046). Four fungal strains which are identified as indicator fungi, able to decolourize effectively were selected for the decolourization and degradation in vitro experimental work. They are Aspergillus erythrocephalus (NCBT 124), Aspergillus fumigates (NCBT 126), Cladosporium herbarum (NCBT 142) and Fusarium oxysporum (NCBT 156). The physico-chemical analysis of bacterial and fungal consortia mediated textile effluent degradation process have shown reduction in all these parameters tested for the untreated textile effluent. Between bacterial consortium and fungal consortium mediated degradation process, the fungal consortium mediated degradation process has shown much reduction in all the parameters than the bacterial consortium mediated degradation. The genotoxicity studies in relation to mitotic index and chromosomal abnormalities have shown gradual increase in active mitotic index and reduction in the chromosomal abnormalities which ensures the reduction in toxicity of textile effluent by bacterial and fungal consortia degradation.


  1. WHO (World Health Organization) (2001)., Guideline for drinking water quality., World Health Organization Press, Geneva.
  2. Mohabansi N.P., Tekade P.V. and Bawankar S.V. (2011)., Physico-chemical parameters of textile mill effluent, Hinganghat, Dist. Wardha (MS)., Current World Environment, 6(1).
  3. Rohilla S.K., Salar R.K. and Kumar J. (2012)., Optimization of physiochemical parameters for decolourization of reactive Black HFGR using soil fungus., Aspergillus allhabadii MTCC 9988, Journal of Bioremediation and Biodegradation, 3, 1-5.
  4. Prasad A. and Rao K.V.B. (2011)., Physicochemical analysis of textile effluent and decolorization of textile azo dye by Bacillus Endophyticus strain VITABR13., Environ. Biotechnol, 2(2), 55-62.
  5. Furaha M.C., Kelvin M.M. and Karoli N.N. (2015)., Assessment of Heavy Metals in Treated Wastewater Used for the Irrigation of Vegetable Plants in Arusha City., International Journal of Research Chemistry and Environment, 5(1), 54-60.
  6. Asfaw A. (2014)., Heavy metals concentration in tannery effluents, associated surface water and soil at Ejersa area of East Shoa, Ethiopia., Herald J Geogr Reg Plan, 3(3), 124-130.
  7. Maruthi Y.A. and Rao S.R. (2001)., Effect of Sugarmill Effluent on Organic Reserves of Fish., Pollution Research, 20(2), 167-171.
  8. Kumar R.S., Swamy R.N. and Ramakrishnan K. (2001)., Pollution studies on sugar mill effluent-physico-chemical characteristics and toxic metals., Pollution Research, 20(1), 93-97.
  9. Jamaluddin Ahmed M. and Nizamuddin M. (2012)., Physico-chemical assessment of Textile Effluents in Chittagong region of Bangladesh and their possible effects on environment., International Journal of Research in Chemistry and Environment, 2, 220-230.
  10. Okunade D. A. and Adekalu K. O. (2013)., Physico-chemical analysis of contaminated water resources due to Cassava wastewater effluent disposal., European International Journal of Science and Technology, 2(6), 75-84.
  11. Basha S.A. and Rajaganesh K. (2014)., Microbial bioremediation of heavy metals from textile industry dye effluents using isolated bacterial strains., Int. J. Curr. Microbiol. Appl. Sci, 3, 785-794.
  12. Faryal R. and Hameed A. (2005)., Isolation and characterization of various fungal strains from textile effluent for their use in bioremediation., Pak. J. Bot., 37(4), 1003-1008.
  13. Rajeswari K., Subashkumar R. and Vijayaraman K. (2013)., Physico-chemical parameters of Effluents collected from Tirupur Textile dyeing and CETP and analysis of Heterotropic bacterial population., Journal of Microbiology and Biotechnology Research, 3, 37-41.
  14. Azbar N., Yonar T. and Kestioglu K. (2004)., Comparison of various advanced oxidation processes and chemical treatment methods for COD and colourremoval from a polyester and acetate fiber dyeing effluent., Chemosphere, 55, 35-43.
  15. Mahawar P. and Akhtar A. (2015)., Physico-chemical characterization of soil and effluent of dye industries in Kaithun region of Kota Rajasthan., Int J Pure Appl Biosci, 3(2), 419-422.
  16. Gangwar Deepali K.K. (2011)., Bioremediation of chromium (VI) from textile industry's effluent and contaminated soil using Pseudomonas putida., Iranica Journal of Energy and Environment, 2, 24-31.
  17. Gabr R.M., Hassan S.H.A. and Shoreit A.A.M. (2008)., Biosorption of lead and nickel by living and non-living cells of Pseudomonas aeruginosa ASU 6a., International Biodeterioration & Biodegradation, 62(2), 195-203.
  18. Joshi V.J. and Santani D.D. (2012)., Physicochemical Characterization and Heavy Metal Concentration in Effluent of Textile Industry., Universal Journal of Environmental Research and Technology, 2(2), 93-96.
  19. Sabour B., Loudiki M., Oudra B., Vasconcelos V., Martins R., Oubraim S. and Fawzi B. (2002)., Toxicology of a Microcystis ichthyoblabe waterbloom from lake oued Mellah (Morocco)., Environmental Toxicology: An International Journal, 17(1), 24-31.
  20. Malik A., Khan I. F. and Aleem A. (2002)., Plasmid incidence in bacteria from agricultural and industrial soils., World J Microbiol Biotechnol, 18(9), 827-833.
  21. Jaishree and Khan T.I. (2014)., Physico-chemical analysis of contaminated soil collected from different areas of Sanganer textile industries Jaipur (Rajasthan)., International Journal of Geology, Earth and Environmental Sciences, 4, 216-219.
  22. Joshi N. and Kumar A. (2011)., Physico-chemical Analysis of Soil and Industrial Effluents of Sanganer Region of Jaipur Rajasthan., Res J of Agri Sci, 2(2), 354-356.
  23. Mathur N., Bhatnagar P. and Verma H. (2006)., Genotoxicity of vegetables irrigated by industrial waste water., J of Environ Sci, 18(5), 964-968.
  24. Bianchi J., Fernandes T.C.C. and Marin-Morales M.M.A. (2016)., Induction of mitotic and chromosomal abnormalities on Allium cepa cells by pesticides imidacloprid and sulfentrazone and the mixture of them., Chemosphere, 144, 475-483.
  25. Kuchy A.H., Waire A.A. and Kamili A.N. (2016)., Cytogenetic effects of three commercially formulated pesticides on somatic and germ cells of Allium cepa., Environ. Sci. Pollut. R., 23(7), 6895-6906.
  26. de Campos Ventura-Camargo B., de Angelis D.D.F. and Marin-Morales M.A. (2016)., Assessment of the cytotoxic, genotoxic and mutagenic effects of the commercial black dye in Allium cepa cells before and after bacterial biodegradation treatment., Chemsphere, 161, 325-332.
  27. Tartar G., Kaymark F. and Murati F.D.G. (2006)., Genotoxic effects of avenoxan on Allium cepa L. and Allium sativum L., Caryologia, 59, 241-247.
  28. Mustafa Y. and Arikan E. S. (2008)., Genotoxicity testing of Quizatofop-p-ethyl herbicide using the Allium cepa anaphase-telophase chromosome aberration assay., Caryologia, 61, 45-52.
  29. APHA. (2005)., Standard methods for the examination of water and waste water., 21st Ed. American Public Health Association, Wahington DC.