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Lead-induced Oxidative Stress and Metabolic Alteration in Seedlings of Brassica juncea L.

Author Affiliations

  • 1Department of Post-Graduate Studies and Research in Botany, Gulbarga University, Gulabarga, 585106, Karnataka, India
  • 2Department of Post-Graduate Studies and Research in Botany, Gulbarga University, Gulabarga, 585106, Karnataka, India

Int. Res. J. Environment Sci., Volume 5, Issue (3), Pages 37-41, March,22 (2016)

Abstract

The present study was conducted to assess lead toxicity causes metabolic alteration and oxidative stress in seedlings of Brassica juncea L. by petri-dish experiment. Surface sterilized seeds were germinated with graded concentrations (10, 20, 30 and 40 mM) of lead nitrate in petriplates lined with Whatman no.1 filter paper. Lead stress resulted, a uniform decrease in germination (100-10%), biomass (0.59-0.08mg/fw and 0.05-0.01mg/dw), root (7.20-0.61cm) and shoot (8.12-1.15cm) elongation with increasing concentrations marked as the primary signs of lead injury. An increasing concentration of lead treatment showed a uniform decrease in chlorophylls (16.94-6.10mg/gm) and &

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