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Isolation and Characterization of Halotolerant Bacillus sp with extra Cellular A-Amylase production Potential from Sambhar salt lake, India

Author Affiliations

  • 1 Department of Zoology, J.N.V.U., Jodhpur, Rajsthan, INDIA

Int. Res. J. Environment Sci., Volume 4, Issue (6), Pages 7-10, June,22 (2015)

Abstract

Sambhar lake (27 58 N, 75 55 E) is a halo-alkaline playa with water density varying from 3-27 Be (Baume). The lake was explored for the diversity of Halophilic microbes. The study led to the isolation of a Moderate halophilic bacterial strain from brine samples of the lake. The isolate showed a growth range of 3-15% NaCl in alkaline medium and was designated as SL-3. The isolate was aerobic, gram +ve, spore forming, rod-shaped and motile. It also tested positive for catalase and oxidase. The isolate was tested for exo-enzyme production and found positive for -Amylase and negative for lipase and protease production. The Isolates was not able to utilize citrate as a sole carbon source and failed to produce HS in TSI-medium. Carbohydrate fermentation revealed utilization of glucose, fructose and sucrose. Taxonomic Identification of isolate was confirmed by partial 16s rRNA gene sequence analysis that contained 1197 nucleotides. The phylogenetic study revealed that the isolate matches 100% with Bacillus licheniformis strain PS-6. Therefore the isolated bacterial strain belongs to phylum Firmicutes with low G+C contents and Genus Bacillus. Gene Bank Accession No. for the isolate is KP172152.

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