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Biodegradation of Dye Reactive Black-5 by a Novel Bacterial Endophyte

Author Affiliations

  • 1 Department of Botany, University of Rajasthan, Jaipur, INDIA

Int. Res. J. Environment Sci., Volume 4, Issue (4), Pages 44-53, April,22 (2015)


The dye Reactive Black-5 was observed to be decolorized and degraded efficiently by a novel bacterial endophyte isolated from plant Amaranthus spinosus, collected from the site contaminated with effluents from textile dyeing and printing industries in Sanganer, Jaipur, Rajasthan. The16SrDNA analysis identified the bacterial endophyte to be Exiguobacterium profundum strain N4. The Exiguobacterium profundum strain N4 efficiently decolorized 901ppm of dye upto 84.78 % after 12 hrs of incubation under static condition at 34C. Beef extract upto 2g/ltr. and glucose upto 10 ml/ltr were found to be most favorable for attaining maximum biodecolorization with the isolated bacterial endophyte. UV–Vis spectroscopy, HPLC and GC-MS analysis confirmed biodegradation of the azo dye. A probable biodegradation pathway was proposed with the help of GC-MS analysis. Phytotoxicity studies with seeds of the plants Triticum aestivum and Phaseolus mungo, confirmed non-toxic nature of the extracted metabolites, while the dye exhibited toxicity.


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