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Identification and Characterization of Microbes from Industrial area for their Heavy metal Tolerance against Cadmium, Lead and Mercury

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Author Affiliations

  • 1 Nitza Biologicals (P) Ltd., Secunderabad, 500056, Telangana, INDIA
  • 2 Siksha O Anusandhan University, Khandagiri, Bhubaneswar, 751003, Odisha, INDIA
  • 3 Trident Academy of Creative Technology, Patia, Bhubaneswar, 751024, Odisha, INDIA

Int. Res. J. Environment Sci., Volume 4, Issue (4), Pages 64-73, April,22 (2015)

Abstract

The search for bacteria and fungus capable against metal toxicity starts with their isolation from the waste water released from the industries that uses either the heavy metals in their process or releases heavy metals as their waste product. The waste effluent released from paper, textile, paint and iron processing industries were collected and different microbial colonies were isolated from those waste water by standard plating methods, identified by their colony morphology, staining methods and different biochemical procedure. Those isolates were then screened for their antibiotics sensitivity and heavy metal toxicity test. From the antibiotics sensitivity test, Erythromycin and Streptomycin proved to be better antibiotics against isolated bacteria and Tetracycline and Ampicillin proved to be better against fungal isolates. Those antibiotics can be used as good selection markers in the molecular biology techniques. For heavy metal toxicity test, three heavy metals such as Cadmium, Mercury and Lead were analyzed at different concentrations such as 1mM, 5mM, 10mM and 20mM for up to 72 hours for bacterial isolates and 144 hours for fungal isolates. The potential isolates were selected over their growth rate at higher concentration of heavy metals. Bacterial isolates such as Bacillus megaterium, Bacillus licheniformis, Pseudomonas fluoroscence, Pseudomonas syringae, Bacillus subtilis, Corynebacterium xerosis, Bacillus macerans and fungal isolates such as Fusarium, Aspergillus niger, Aspergillus flavus, Cladosporium proved to be the better isolates that can be exploited at their molecular level for the bioremediation of heavy metal contamination.

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