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Method Validation and Uncertainity measurement for Determination of 25 Pesticides by GC-ECD and MSD in Banana using modified QuEChERS Technique

Author Affiliations

  • 1 Institute of Pesticide Formulation Technology, Sector-20, Udyog Vihar, Opp. Ambience Mall, NH-8, Gurgaon, Haryana-122016, INDIA

Int. Res. J. Environment Sci., Volume 4, Issue (3), Pages 21-27, March,22 (2015)

Abstract

Method for the determination of multi-residue was developed by slight modification in QuEChERS method and validated for 25 pesticides of different class in banana. Extraction of samples was done with acetonitrile and cleaning by PSA and 18 using Solid Phase Extraction technique Recovery studies at three spiking concentration level 1 LOQ (Limit of quantification), 5 LOQ and 10 LOQ varied from 74 % to 117 % with Relative Standard Deviation (RSD) below 20%. Measurement of uncertainity (MU), for three main independent sources viz. weighing, purity of the standard and repeatability were considered. The method developed can be used as one single determination step for the analysis of all 25 pesticides by GC-ECD and their confirmation by GC-MS.

References

  1. Eskenazi B., Rosas L.G., Marks A.R., Bradman A. And Harley K., Pesticide toxicity and the developing brain, BasicClin. Pharmacol. Toxicol., 102, 228-236 (2008)
  2. Fernndez-Alba A.R. and Garca-Reyes J.F., Large-scale multi-residue methods for pesticides and their degradation products in food by advanced LC-MS, Trac-Trend. Anal. Chem., 27(11), 973-990 (2008)
  3. Ecobichon D.J., Pesticide use in developing countries, Toxicology, 160(1–3), 27-33 (2001)
  4. Handa S.K., Agnihotri N.P. and Kulshrestha G., Pesticides Residues: Significance Management and Analysis, Research Periodicals and Book Publishing Home, Texas: USA, 138-140, (1999)
  5. Belardi R.P. and Pawliszyn J., The application of chemically modified fused silica fibresin the extraction of organics from water matrix samples and their rapid transfer to capillary columns, Water Pollut. Res. J. Can., 24, 179, (1989)
  6. Dasika R., Tangirala S. and Naishadham P., Pesticide residue analysis of fruits and vegetables, Journal of Environmental Chemistry and Ecotoxicology, 4(2), 19-28, (2012)
  7. Durovic R., Markovic M. and Markovic D., Headspace solid phase microextraction in the analysis of pesticide residues – kinetics and quantification prior to the attainment of partition equilibrium, Journal of the Serbian Chemical Society, 72, 879-887, (2007a)
  8. Luo L., Shao B. and Zhang J., Pressurized liquid extraction and cleanup procedure for the determination of pyrethroids in soils using gas chromatography/tandem mass spectrometry, Analytical Sciences, 26(4), 461-465, (2010)
  9. Di Muccio A., Fidente P., Attard Barbini D., Dommarco R., Seccia S., Morrica P., Application of solid-phase extraction and liquid chromatography–mass spectrometry to the determination of neonicotinoid pesticide residues in fruit and vegetables, J. of Chromatogr. A., 1108(1), 1-6 (2006)
  10. Lambropoulou D.A. and Albanis T.A., Methods of sample preparation for determination of pesticide residues in food matrices by chromatography–mass spectrometry-based techniques: a review, Analitycal and Bioanalytical Chemistry., 389(6), 1663-1683 (2007)
  11. Schenck F.J., Lehotay S.J. and Victor V., Comparison of solid-phase extraction sorbents for cleanup in pesticide residue analysis of fresh fruits and vegetables, Journal of Separation Science, 25(14), 883–890 (2002)
  12. Pawliszyn J., Solid phase microextraction: theory and practice, Wiley-VCH, New York, USA, (1997)
  13. Anastassiades M., Lehotay S.J., Stajnbaher D. and Schenck F.J., Fast and easy multiresidue method employing acetonitrile extraction/partitioning and “dispersive solidphase extraction” for the determination of pesticide residues in produce, J. AOAC. Int.,86, 412-431, (2003)
  14. Reed D.V., Lombardo P., Wessel J.R., Burke J.A. andMacmohan B., The FDA pesticides monitoring programme, J.AOAC. Int.,70, 591-595 (1987)
  15. Fong W.G., Moye H.A., Seiber J.N. and Toth J.P., Pesticide residues in foods, Methods, techniques, and regulations, John Wiley and Sons, New York, USA, 139-209 (1999)
  16. Kumazawata T. and Suzuki O., Separation methods for amino group-possessing pesticides in biological samples, J. Chromatogr. B.,747, 241–254 (2000)
  17. Geerdink R.B., Niessen WMA. and Brinkman UATh., Trace-level determination of pesticides in water by means of liquid and gas chromatography, J. Chromatogr. A., 970, 65–93 (2002)
  18. Corley J., Best practices in establishing detection and quantification limits for pesticide residues in foods, Hand book of Residue Analytical Methods for Agrochemicals, Wiley and Sons, New York, USA, 59-74, (2003)
  19. EURACHEM/CITAC Guide CG 4, EURACHEM/ CITAC Guide, Quantifying uncertainity in analytical measurement, 2nd ed. http://www. measurementuncertainity.org, (2000)