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Desiccation tolerance in Artillery Plant (Pilea microphylla (L.) Liebm): A search

Author Affiliations

  • 1Plant Biochemistry and Molecular Biology Laboratory, Department of Botany, University college, Trivandrum, 695 034, Kerala, INDIA

Int. Res. J. Environment Sci., Volume 4, Issue (12), Pages 26-32, December,22 (2015)


Water deficiency is the most significant abiotic stress factor for land plants. Most plants are unable to survive desiccation to the air dry state. There are however a few species from lower plant groups to flowering plants that tolerate desiccation known as resurrection plants. So, the present investigation was undertaken to study the biochemical changes in Pilea microphylla against desiccation (1, 3, 5 and 7 days) and rehydration (30 min) stress. As an initial part of the study total sugar, protein and proline content were analyzed and showed a gradual decline during the stress periods. The SDS-PAGE analysis of protein indicated the disappearance of certain bands in the desiccated and rehydrated samples (7D) when compared to the control indicating the denaturation of proteins during stress. Similarly, the appearance of new bands (15kDa on 3D and 20 and 17kDa on the 5D) were also noticed suggesting the formation of stress related proteins to tide over desiccation. The amount of free amino acids increased in P. microphylla, in pace with desiccation periods. Constitutive increase in the level of proline (the stress amino acid) accumulation is seen when compared with the control i.e., 6 fold higher than that of the control, after exposed to desiccation for 5 D. Increase in proline level relating to exposure time of desiccation stress suggests its role as osmolyte. Similarly, a reduction in chlorophyll level and an increase in carotenoid were also observed during stress. Decrease of total chlorophyll content was observed with duration of desiccation. The decrease in chlorophyll a and b was recovered during rehydration up to 5 D. Further studies are warranted at molecular level in terms of stress proteins and genes involved in desiccation tolerance in this plant.


  1. Tuba Z., Proctor M.C.F. and Csintalan Z.S., Ecophysiological responses of homoiochlorophyllous and poikilochlorophyllous desiccation tolerant plants: a com-parison and an ecological perspective, Plant Growth Regul., 24, 211-217 (1998)
  2. Chappelle E.W., Kim M.S. and McMurtrey J.E.I., Ratio analysis of reflectance spectra (RARS): an algorithm for the remote estimation of the concentrations of chlorophyll , chlorophyll , and carotenoids in soybean leaves, Rem. S. Environ., 39, 239-247 (1992)
  3. Miller G. L., Analytical Chemistry, New York: McGraw Hill Book, 426, 1972)
  4. Moore S. and Stein W.H., Photometric ninhydrin method for use in the chromatography of amino acids, J. Biol. Chem.,176, 367388 (1948)
  5. Bates L. S., Waldron R. P. and Teare I. D., Rapid determination of free proline for water stress studies, Plant and Soil, 39, 205-208 (1973)
  6. Lowry O.H., Rosebrough N.J., Farr A.L. and Randall R.J., Protein measurement with folin phenol reagent, J. Biol. Chem.,193, 265-295 (1951)
  7. Laemmli U.K., Clevage of structural proteins during the assembly of the head of bacteriophage T4, Nature,227, 680-685 (1970)
  8. Fairbanks V.F., Fahey J.L. and Beutter E., Clinical disorders of iron metabolism Clinical 2nd Ed. Grune and Stratton N.Y Stratton, Inc: Newyork (1971)
  9. Georgieva K., Sarvari E. and Keresztes A., Protection of thylakoids against combined light and drought by a luminal substance in the resurrection plant Haberlea rhodopensis, Ann. Bot., 105, 117126 (2010)
  10. Oliver M.J., Velten J. and Mischler B. D., Desiccation tolerance in bryophytes: a reflection of a primitive strategy for plant survival in dehydrating habitats, Integr. Comp. Biol.45, 788-799 (2005)
  11. Anderson J. M., Photoregulation of the composition, function, and structure of thylakoid membranes, Ann. Rev. Plant Physiol., 37, 93136 (1986)
  12. Beer S., Bjork M., Hellblom F. and Axelsson L., Inorganic carbon utilization in marine angiosperms (sea grasses), Funct. Plant Biol., 29, 349354 (2002)
  13. Ma J. F., Zheng S. J., Hiradate S. and Matsumoto H., Detoxifyingaluminum with buck wheat, Nature, 390, 569-570 (1997b)
  14. Rao G. G. and Rao G. R., Pigment composition and chlorophyllase activity in pigeon pea (Cajanusindicus Spreng) and Gingelley (Sesamum indicum L.) under NaCl salinity, Ind. J. Expt. Biol., 19, 768-770 (1981)
  15. Singh A. K. and Dubey R. S., Changes in chlorophyll a and b contents and activities of photosystems I and II in rice seedlings induced by NaCl, Photosynthetica, 31, 489-499 (1995)
  16. Horton ruban A. V. and Walters R. G., Regulation of light harvesting in green plants, Ann. Rev. Plant Physiol. Plant Mol. Biol., 47, 655684 (1996)
  17. Marschall M. and Proctor M. C., Are bryophytes shade plants? Photosynthetic light responses and proportions of chlorophyll , chlorophyll b and total carotenoids, Ann. Bot., 94, 593-603 (2004)
  18. Leong T. Y. and Anderson J. M., Changes in composition and function of thylakoid membranes as a result of photosynthetic adaptation of chloroplasts from pea plants grown under different light conditions, Biochimica et BiophysicaActa, 723, 391-399 (1983)
  19. Hendry G.A.F., Evolutionary origins and natural functions of fructans: a climatological, biogeography and mechanistic appraisal, New Phytologist, 123, 3-14 (1993)
  20. Livingston D.P., Hincha D.K. and Heyer A.G., Fructan and its relationship to abiotic stress tolerance in plants, Cell. Mol. Life Sci., 66, 2007-2023 (2009)
  21. Spollen W.G. and Nelson C.J., Response of fructan to water-deficit in growing leaves of tall fescue, Plant Physiol.,106, 329-336 (1994)
  22. Olien C.R. and Clark J.L., Freeze-induced changes in carbohydrates associated with hardiness of barley and rye, Crop Sci.,35, 496-502 (1995)
  23. Basu P.S., Ali M. And Chaturvedi, S.K., Osmotic adjustment increases water uptake, remobilization of assimilates and maintains photosynthesis in chickpea under drought, Ind. J. Expt. Biol., 45, 261-267 (2007)
  24. Kempa S., Krasensky J., Dal Santo S., Kopka J. and Jonak C., A central role of abscisic acid in stressregulated carbohydrate metabolism, PLoS One, e3935 (2008)
  25. Kaplan F. and Guy C.L., Beta-Amylase induction and the protective role of maltose during temperature shock, Plant Physiol.,135, 1674-1684 (2004)
  26. Davies K.J.A., Protein damage and degradation by oxygen radicals. I. General aspects, J Biochem Chem.,262, 98959901 (1987)
  27. Girousse C., Bournoville R. and Bonnemain J. L., Water deficit-induced changes in concentrations in proline and some other amino acids in the phloem sap of alfalfa, Plant Physiol.,111, 109-113 (1996)
  28. Sharma S. S. and Dietz K. J., The significance of amino acids and amino acid-derived molecules in plant responces and adaptation to heavy metal stress, J. Expt. Bot.,57, 711-726 (2006)
  29. Delauney A. J. and Verma D. P. S., Proline biosynthesis and osmoregulation in plants, Plant J., 215-223 (1993)
  30. Eder A., Hubber W. and Sankhla N., Interaction between salinity and ethylene in nitrogen metabolism of Pennisetum typhoides seedling, Biochemie und Physiologie der Pflanzen, 171, 93-100 (1977)
  31. Chen T. H. and Murata N., Enhancement of tolerance of abiotic stress by metabolic engineering of betaines and other compatible solutes, Current opinion in Plant Biol., 250257 (2002)
  32. Girija C., Smith B. N. and Swamy P. M., Interactive effects of sodium chloride and calcium chloride on the accumulation of proline and glycinebetaine in peanut Arachishypogaea L.), Environ. Expt. Bot.,43, 110 (2002)
  33. Yadav S. K., Jyothilakshmi N., Maheswari M., Vanaja M. and Venkateswarlu B., Influence of water deficit at vegetative, anthesis and grain filling stages on water relation and grain yield in sorghum, Indian. J. Plant Physiol., 10, 2024 (2005)
  34. Nath A. K., Kumari S. and Sharma D. R., In vitro selection and characterization of water stress tolerant cultures of bell pepper, Ind. J. Plant Physiol., 10, 1419 (2005)
  35. Kasturibai K. V. and Rajagopal V., Osmotic adjustment as a mechanism for drought tolerance in coconut (Cocosnucifera L.), Ind. J.Plant Physiol., 320-323 (2000)
  36. Hamada A. M., Amelioration of drought stress by ascorbic acid, thiamin and aspirin in wheat plants, Ind. J. Plant Physiol.,, 358-364 (2000)
  37. Asha S. and Rao K. N., Effect of simulated water logging on the levels of amino acids in groundnut at the time of sowing, Ind. J. Plant Physiol.,, 288-291(2002)
  38. Morgan J. M., Osmoregulation and water stress in higher plants, Ann. Rev. Plant Physiol.,33, 299319 (1984)
  39. Jaleel C. A., Gopi R., Sankar B., Manivannan P., Kishorekumar A., Sridharan R. and Panneerselvam R., Studies on germination, seedling vigour, lipid peroxidation and proline metabolism in Catharanthus roseus seedlings under salt stress, South African J. Bot., 73, 190-195 (2007c)
  40. Chandrasekar K. R. and Sandhyarani S., Salinity induced chemical changes in Crotalaria striata DC. Plants, Ind. J. Plant Physiol., 4448 (1996)
  41. Demir Y., Growth and proline content of germinating wheat genotypes under ultra violet light, Turk. J. Bot.,24, 67-70 (2000)
  42. Lin C. C., Hsu Y. T. and Kao C. H., The effect of NaCl on proline accumulation in riceleaves, Plant Growth Regul.,36, 275285 (2002)
  43. Tripathi B. N. and Gaur J. P., Relationship between copper and zinc induced oxidative stress and proline accumulation in Scenedesmus sp., Planta, 219, 397-404 (2004)
  44. Larosa P. C., Rhodes D., Rhodes J. C., Bressan R. A. and Csonka L. N., Elevated accumulation of proline in NaCl adapted tobacco cells is not due to altered pyrroline- 5-carboreylate reductase, Plant Physiol., 96, 245250 (1991)
  45. Bray E. A., Drought-stress-induced polypeptide accumulation in tomato leaves, Plant Cell and Environ., 13, 531538 (1990)
  46. Fujita T., Maggio A., Rios M. G., Stauffache R. C., Bressan R. A. and Csonka L. N., Identification of regions of the tomato g-glutamyl kinase that are involved in allosteric regulation by proline, J. Biol. Chem., 278, 1420314210 (2003)
  47. Hartl F.U., Molecular chaperones in cellular protein folding, Nature,381, 571-580 (1996)
  48. Frydman J., Folding of newly translated proteins in vivo: the role of molecular chaperones, Ann. Rev. Biochem., 70, 603- 647 (2001)
  49. Ali Ahmad M. and Basha S.M., Effect of water stress on composition of peanut leaves, Peanut Sci.,25, 31-34(1998)
  50. Jyoti Ranjan Rout. and Santi Lata Sahoo., Morphological and Protein Profile Alterations in Withinia somnifera L. with response to iron stress, Ind. J. Life Sci.,2(1), 21-25 (2012)