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Kinetic Study on Biogas Production from Fish Pond Effluent co-digested with Cow dung in a Batch Bioreactor system

Author Affiliations

  • 1Department of Microbiology, Federal University of Technology, P.M.B.1526 Owerri, Imo State, NIGERIA
  • 2 Department of Microbiology, Imo State University, Owerri, NIGERIA

Int. Res. J. Environment Sci., Volume 4, Issue (12), Pages 1-7, December,22 (2015)


This study evaluated biogas production from fish pond effluent co-digested with cow dung using cow rumen microorganisms as the inoculum. The four (4) batch bioreactors of ten (10) litre capacity used were operated at ambient temperature (26-35℃) and pH range of 6.5 - 8.5 for 33 days. The bioreactors were charged with different ratios of fish pond effluent (FPE) and cow dung (CD); 2.5L/400g; 2.5L/ 500g; and 2.5L/600g; for digester 1, 2 and 3, respectively while digester 4 (control) contained 2.5L of the FPE. The Total volatile solid (TVS) of the seeding sludge were 364.1g in bioreactor 1, 493g in 2, 512g in 3 and 74g in 4 (control). Fresh cow rumen liquor (20%) strained with cheesecloth was used as inoculum which provided the source of the methanogens. In order to optimize the pH of the substrate, 3000mg /L of sodium hydrogen carbonate (NaHCO3) was added into the charged bioreactors. Daily biogas yield was measured by the downward water displacement method. Statistical analysis (T test P ≤5%) indicated a significant difference in biogas yield in all the test parameters compared to the control. Significant difference in biogas was also recorded between FPE/400g CD and FPE/600gCD.The cumulative biogas production observed in bioreactor charged with FPE/400g CD, FPE/500g CD and FPE/600g CD were (19.514dm); (21.30dm3) and (25.47dm3), respectively. The bioreactor charged with FPE/600gCD exhibited the highest performance in the production of biogas. Though it demonstrated the highest biogas production potential (Ym), 304.10 ml/gVS but the maximum biogas production rate (U) was exhibited by FPE/ 400g CD, 4.33 ml/ g VS/day.The modified Gomperzt equation properly construes the cumulative biogas produced as a function of time.


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