International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Activities of selected Enzymes in various Tissues of Pila Globosa under Heat Shock

    , ,

Author Affiliations

  • 1 Department of Biosciences, Sri Sathya Sai Institute of Higher Learning, Prasanthi Nilayam Campus, Puttaparthi, Anantapur Dist., AP, INDIA

Int. Res. J. Environment Sci., Volume 3, Issue (6), Pages 11-16, June,22 (2014)

Abstract

The physiology of Pila globosa (Indian apple snail) under heat shock was analyzed by measuring activities of two anti-oxidative enzymes and one oxidative enzyme in selected tissues. The temperature set points for the experiments were based on the climatic variations the snail gets exposed during an annual period in the specific geographical region. From the tissues of hepatopancreas, gills and foot, the enzyme activities were assessed to understand the compensatory mechanisms the snail utilizes by physiological adjustments. The results indicated that catalase (CAT), Glutathione-S-transferase (GST) and Lactate dehydrogenase (LDH) had tissue specific variations and play role in homeostasis. The study assumes significance with rising temperatures on earth due to global warming and also supports in understanding adaptive capabilities of these amphibious snails.

References

  1. Gordon M.R., McLeay D.J., Effect of photoperiod on seasonal variations in glycogen reserves of juvenile rainbow trout Salmo gairdneri, Comp. Biochem. Physiol. A: Physiol., 60, 349-351 (1978)
  2. Halliwell B., Free radicals and antioxidants: updating a personal view, Nutr. Rew.,70(5), 257-65(2012)
  3. Krupanidhi S., Raghavaiah K., B.P., N.; Ramamurthi R., Oxidation of glucose-U-14 c and synthesis of glycogen in different tissues of the garden snail, Cryptozona ligulatawith reference to aestivation and starvation, Proc Indian natn SciAcad., 87B, 27-31 (1978)
  4. Devi S.A., Effects of Aestivation and Rehabilitation on Tissue Catalase Activity of Pila globosa, Proc. Ind. Natn. Sci.Acad., B49, 610-612 (1983)
  5. Pannunzio, Antioxidant defenses and lipid peroxidation during anoxia stress and aerobic recovery in the marine gastropod Littorina littorea J. Exp. Mar. Biol. Ecol., 221, 277–292(1998)
  6. Beers R.F. and Sizer I.W., A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase, J. Biol. Chem, 195(1), 133-40 (1952)
  7. Pabst M.J., Habig W.H. and Jakoby W.B., Glutathione S-transferase A. A novel kinetic mechanism in which the major reaction pathway depends on substrate concentration, J. Biol. Chem., 249(22), 7131-7139 (1974)
  8. Bishop M.J., Everse J. and Kaplan N.O., Identification of lactate dehydrogenase isoenzymes by rapid kinetics, Proc. Natl. Acad. Sci. U S A., 69(7), 1761-5 (1972)
  9. Bhandari G., Study on Climate Change Impacts and Adaptation Measures in Palpa District of Nepal, Int. Res. J. Environmen Sci.,2(3), 15-23 (2013)
  10. Warade N.V., Levels of Glutathione S - Transferase In Different Larval Tissues of Papilio demoleus,Res. J. Recent Sci.,1(ISC-2011)313-316 (2012)
  11. Malanga G., Estevez M.S., Calvo J. and Puntarulo S., Oxidative stress in limpets exposed to different environmental conditions in the Beagle Channel. Aquat. Toxicol.,69(4), 299-309 (2004)
  12. Abele D., Burlando B., Viarengo A., Portne H.O., Exposure to elevated temperatures and hydrogen peroxide elicits oxidative stress and antioxidant response in the Antarctic intertidal limpet Nacella concinna, Comp. Biochem. Physiol. B.,120425–435 (1998)
  13. Malanga G., Estevez M.S., Calvo J., Abele D. and Puntarulo S., Oxidative stress in gills of limpets from the Beagle Channel: comparison with limpets from the Antarctic, Sci.Mar., 69(S2), 297-304 (2005)
  14. Verlecar X.N., Jena K.B. and Chainy G.B., Biochemical markers of oxidative stress in Perna viridis exposed to mercury and temperature, Chem. Biol. Interact.,167 (3), 219-26 (2007)
  15. Lushchak V.I. and Bagnyukova T.V., Effects of different environmental oxygen levels on free radical processes in fish, Comp. Biochem. Physiol. B., 144(3), 283-9 (2006)
  16. Malleswar D., Pila globosa (Indian Apple Snail) a Sentinel Organism to Monitor Climatic Stress in the Ecosystem: Mechanisms of Adaptation. Ph.D thesis., Sri Sathya Sai Institute of Higher learning, Prasanthi Nilayam, (2013)
  17. Findley R.C., Gillies R.J. and Shulman R.G., In vivo phosphorous 31 NMR reveals lowered ATP during heat shock of tetrahymena, Science, 245(385), 39 (1983)
  18. Wieser W., The initial stage of anaerobic metabolism in the snail, Helix pomatia L., FEBS Lett.,95(2), 375-8 (1978)