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Acetylcholinesterase activities in the Nervous system of snail Lymnaea acuminata as Biomarkers of Water pollutants in Ramgarh Lake, Gorakhpur, UP, India

Author Affiliations

  • 1 Department of Applied Science and Humanities, Buddha Institute of Technology, Gida, Gorakhpur, UP, INDIA

Int. Res. J. Environment Sci., Volume 3, Issue (2), Pages 20-26, February,22 (2014)


The effect of different environmental factors in different season and their relative effect on acetylcholinesterase (AChE) enzyme in the nervous tissue of snail Lymnaea acuminate were determined during various months of the year (2007-2008). Temperature, pH, dissolved oxygen (DO), carbon dioxide (CO), conductivity and turbidity were measured in control (tap water) as well as polluted water (Ramgarh lake) was measured simultaneously, that such ,the nervous tissue of the snail was assayed for the activity of acetylcholinesterase at 24h and 96h, physical and chemical parameters of the twelve months of the same year. Highest inhibition of AChE was observed in summer season and minimum in winter season. In summer season AChE inhibition was high because temperature, carbondioxide (CO) and turbidity was high and pH, dissolved oxygen (DO), conductivity was low. There was a significant product positive and negative correlation between AChE and pH Temperature, dissolved oxygen (DO), carbondioxide (CO), conductivity and turbidity of water in corresponding months. Analysis of variance (ANOVA) was used to analyze differences in biomarkers activity and each site.


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