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An in vitro hydroponic study on Physiological and Biochemical responses of Indian wild Rice to varying doses of Hexavalent Chromium

Author Affiliations

  • 1Laboratory of Plant Biochemistry and Environmental Biotechnology, Post Graduate Department of Botany. Utkal University, Bhubaneswar-751004, Odisha, INDIA

Int. Res. J. Environment Sci., Volume 3, Issue (11), Pages 20-28, November,22 (2014)


Indian wild rice (Oryza nivara) grown as a weed in most of the cultivated paddy fields is used as an experimental tool in current investigation and studied for its various physiological and biochemical alterations in response to hexavalent chromium. After exposure to varying concentrations of toxic hexavalent chromium, the two cultivars of Oryza nivara i.e. IC-283169 and IC-336684 showed significant changes in its morphobiometric and physiological parameters. The present hydroponic study exhibited deleterious effects on germination, plant height and biomass of 14 days treated seedlings. IC-336684 cultivar of wild rice showed significant reduction in root-shoot length as well as dry and fresh biomass of shoot beyond a treatment dose of 50 μM Cr+6 as compared to IC-283169 cultivar. Growth parameters, chlorophyll and carotenoid content showed 50% reduction with treatment dose of 25 μM Cr+6. More than 80% reduction in all the above parameters were observed at 100 μM Cr+6 treatments. Present preliminary study screens the tolerance and sensitivity of wild rice cultivars to toxic doses of Cr+6. The aim of this article is to give an overview of the impact of varying doses of hexavalent chromium on two cultivars of Indian wild rice and removal of these toxic contaminants from soil by potent application of weeds like Indian wild rice.


  1. Rouphael Y., Cardarelli M., Reab E. and Colla G., Grafting of cucumber as a means to minimize copper toxicity, Env. Exp. Bot.,63, 49-58 (2008)
  2. Cervantes C., Garcia J.C., Devars S., Corona F.G. and Tavera H.L., Carlos Torres-guzman J, Sanchez RM. Interactions of chromium with micro-organisms and plants, FEMS Microb. Rev., 25, 335347 (2001)
  3. McGrath S.P., The uptake and translocation of tri- and hexavalent chromium and effects on the growth of oat in flowing nutrient solution and in soil, New Phytologist, 92, 381390 (1982)
  4. Sharma R.M.S. and Raju N.S., Correlation of heavy metal contamination with soil properties of industrial areas of mysore, karnataka, India by cluster analysis, I. Res. J. Environment Sci.,2(10), 22-27, (2013)
  5. Poschenrieder C., Vazquez M.D., Bonet A. and Barcelo J., Chromium III iron interaction in iron sufficient bean plants. II Ultrasructural aspects, J. Plant Nutr. 14,415-428 (1991)
  6. Barcelo J., Poschenrieder C., Vazquez M.D. and Gunese B., Aluminium phytotoxicity. A challenger for plant scientists, Fertile. Res.,43, 217-223 (1996)
  7. Barcelo J. and Poschenrieder C., Chromium in plants. In: Carati, S., tottarelli, F., Seqmi, P. (ed): Chromium Environment Issues, Francotangati Press, Milan, 101-129(1997)
  8. Panda S.K. and Patra HK., Physiology of chromium toxicity. A review, Plant Physiol. Biochem., 24,10-17 (1997)
  9. Panda S.K. and Patra H.K., Alternation of nitrate reductase activity by chromium ions in excised wheat leaves, Indian J. Agr. Biochem.,11(2), 56-57 (1998)
  10. Panda S.K and Patra H.K., Does Chromium (III) produce oxidative damage in excised wheat leaves ?, J.Plant Biol.,27, 105-110 (2000)
  11. Suderman F.W., Carcinogenic effect of metals, Fed. Proc.,37(1), 4046 (1978)
  12. Khasim I. and Nanda Kumar N.V., Environmental contamination of chromium in agricultural and animal products near chromate industry, Bull. Environ. Contam. Toxicol., 43, 742746 (1989)
  13. Haq1 B.I.U., Nursafura E. B. and Zakia K., Adsorption Studies of Cr (VI) and Fe (II) Aqua Solutions Using Rubber Tree Leaves as an Adsorbent, I. Res. J. Environment Sci., 2(12), 52-56 , (2013)
  14. Pattnaik N., Mohanty M. and Patra H.K., Effect of chelating agents and metal ions on nickel bioavailability and chlorophyll fluorescence response in wheat- Anapproach for attenuation of Ni stress, J. Stress Physiol. Biochem. 8(3), 99-112 (2012)
  15. Mathan J., Mohanty M., Pradhan C. and Patra H.K., Toxicological changes in rice under nickel stress, Biolife.,2(1),363-369 (2014)
  16. Mohanty M and Patra HK, Effect of Chelate assisted Hexavalent Chromium on Physiological changes, Biochemical alterations and Cr Bioavailability in Crop Plants - An in vitro Phytoremediation Approach, Bioremediat. J.16(3), 147155 (2012)
  17. Mohanty M. and Patra H.K., Effect of ionic and chelate assisted hexavalent chromium on mung bean seedlings Vigna radiata L. wilczek. var k-851) during seedling growth, J. Stress Physiol. Biochem,9(2), 232-241 (2013)
  18. Grataq P.L., Polle A., Lea P.J. and Azevedo A., Making the life of leaves metal stressed plants a little easier, Func. Plant Biol., 32, 481-494 (2005)
  19. Dey S.K., Jena P.P. and Kunelu S., Antioxidative efficiency of Triticum aestivum L, exposed to chromium stress, J. Environ. Biol.,30, 539-544 (2009)
  20. Sandman G. and Boger P., Copper mediated lipid peroxidation process in photosynthetic membranes, Plant physiol.,63,797-800 (1980)
  21. Pandey S.N., Growth and biochemical changes in pulse seedlings irrigated with effluent from electroplating industry, J. Appl. Biosci.,34, 79-82 (2008)
  22. Singh D. and Singh A., Chitosan for the Removal of Chromium from Waste Water, I. Res. J. Environment Sci.,1(3), 55-57 (2012)
  23. Singh D. and Singh A., Chitosan for the Removal of Cadmium Rich Water, I. Res. J. Environment Sci., 1(5),81-83 (2012)
  24. Abeer F.A., and Ghadir A. El-C., Adsorption of Citric Acid from Aqueous Solution onto Activated P. LentiscusLeaves, I. Res. J. Environment Sci.,1(4), 7-13 (2012)