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Effect of Process Parameters on Anaerobic Decolourization of Reactive Azo Dyes Using Bacillus licheniformis isolated from Textile effluent contaminated site in Perundurai, India

Author Affiliations

  • 1Department of Microbiology, Periyar University, Periyar Pakalai Nagar, Salem-636011, TN, INDIA

Int. Res. J. Environment Sci., Volume 2, Issue (8), Pages 37-43, August,22 (2013)


A bacterial strain Bacillus licheniformis with remarkable ability to decolourize the reactive azo dyessuch asreactive red, reactive yellow, reactive brilliant red and reactive brilliant blue was isolated from the textile effluent contaminated site.The effluent sample was collected from Infra Tex textile industry, Perundurai in Erode district situated at Tamil nadu state and country of India. Static conditions with 10 g/l glucose, pH 9, 37C, 20% inoculum concentration, 50 mg/l of dye concentration, 3 g/l of NHNO, were considered to be the optimum decolourizing conditions. Bacillus licheniformis grew well in these optimum conditions, resulting in 82% decolourization extent 7 days of incubation. Phenotypic characterization and phylogenetic analysis of the 16S rRNA sequence indicated that the bacterial strain belonged to the genus Bacillus. UV (Ultra Violet) analysis of bacterial isolate suggested that it exhibited decolourizing activity, rather than inactive surface adsorption. This decolourization extent and facile conditions show the potential for this bacterial strain to be used in the biological treatment of textile effluent or dyes.


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