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Microbial Decolorization of Disperse Textile Dye Brown 21 by Enterobacter gergoviae Isolated from Textile Effluent

Author Affiliations

  • 1Department of Microbiology, Bhagwan Mahavir College of Biotechnology, Surat-394220, Gujarat, INDIA

Int. Res. J. Environment Sci., Volume 2, Issue (5), Pages 31-36, May,22 (2013)


Decolorization of Disperse textile dye Brown 21, a very important commercial dye in textile industries was investigated. A dye decolorizing bacterium was isolated from effluent collected from a GIDC, Pandesara, Surat, India. Various physicochemical parameters like pH, temperature, carbon sources and nitrogen sources were optimized for maximum decolorization of dye. Dye decolorization was observed in the range of pH 7.0 to 10.0. Temperature ranges from 25\rC -39\rC was observed as suitable temperature for dye decolorization. The dye was decolorized more than 90% in aerobic culture conditions. The dye can be used as sole source carbon and energy for cell growth. Additional carbon source glucose increases the rate of dye decolorization. Addition of urea also has increased the rate of dye decolorization. These results suggest that isolated bacterium, Enterobacter gergoviae, is suitable for the biological treatment of dye-containing wastewater.


  1. Allegre C., Moulin P., Maisseu M. and Charbit F., Treatment and reuse of reactive dyeing effluents, Journal of membrane science,269(1), 15-34 (2006)
  2. Tehrani-Bagha A., Mahmoodi N. and Menger F., Degradation of a persistent organic dye from colored textile wastewater by ozonation, Desalination,260(1), 34-38 (2010)
  3. Ali H., Biodegradation of synthetic dyes—a review, Water, Air, & Soil Pollution,213(1), 251-273 (2010)
  4. El-Sersy N.A., Bioremediation of Methylene Blue by Bacillus thuringiensis 4 G 1: Application of Statistical Designs and Surface Plots for Optimization, Biotechnology,6(1), 34-39 (2007)
  5. Salar R.K., Rohilla S.K. and Rohilla J.K., Decolorization of Reactive Black HFGR by Aspergillus sulphureus, ISCA Journal of Biological Sciences,1(1), 55-61 (2012)
  6. Kandelbauer A., Erlacher A., Cavaco-Paulo A. and Guebitz G.M., Laccase-catalyzed decolorization of the synthetic azo-dye diamond black PV 200 and of some structurally related derivatives, Biocatalysis and Biotransformation,22(5-6), 331-339 (2004)
  7. Handayani W., Meitiniarti V.I. and Timotius K.H., Decolorization of Acid Red 27 and Reactive Red 2 by Enterococcus faecalis under a batch system, World Journal of Microbiology and Biotechnology,23(9), 1239-1244 (2007)
  8. Joe M.H., Lim S.Y., Kim D.H. and Lee I.S., Decolorization of reactive dyes by Clostridium bifermentans SL186 isolated from contaminated soil, World Journal of Microbiology and Biotechnology,24(10), 2221-2226 (2008)
  9. Butani N., Parekh H. and Saliya V., Biodegradation of Phenol by a Bacterial Strain Isolated From a Phenol Contaminated Site in India, I Research Journal of Environment Sciences,1(1), 46-49 (2012)
  10. Mir T.A., Manderia S. and Manderia K., Influence of dye industrial effluent on physico chemical characteristics properties of soil at Bhairavgarh, Ujjain, MP, India, I Research Journal of Environment Sciences,1(1), 50-53 (2012)
  11. Pourbabaee A.A., Malekzadeh F., Sarbolouki M.N. and Najafi F., Aerobic decolorization and detoxification of a disperse dye in textile effluent by a new isolate of Bacillussp, Biotechnology and Bioengineering,93(4), 631-635 (2006)
  12. Khalid A., Arshad M. and Crowley D.E., Decolorization of azo dyes by Shewanella sp, under saline conditions, Applied microbiology and biotechnology,79(6), 1053-1059 (2008)
  13. Dawkar V.V., Jadhav U.U., Jadhav S.U. and Govindwar S.P., Biodegradation of disperse textile dye Brown 3REL by newly isolated Bacillus sp. VUS, Journal of Applied Microbiology, 1-11 (2008)
  14. Gerorge M. Garrity, Julia A. Bell and Timothy G. Lilburn, Bergey's manual of systematic bacteriology, New York: Springer; (2004)
  15. Agnes M.D.C., Rajeshwari S. and Venckatesh R., Decolorization of Reactive Violet – 2RL Dye by Aspergillus Flavus and Aspergillus Fumigatus from Textile Sludge, International Research Journal of Environment Sciences,1(2), 8-12 (2012)
  16. Namdhari B.S., Rohilla S.K., Salar R.K., Gahlawat S.K., Bansal P. and Saran A.K., Decolorization of Reactive Blue MR, using Aspergillus species Isolated from Textile Waste Water, ISCA Journal of Biological Sciences,1(2), 24-29 (2012)
  17. Wang H., Zheng X.W., Su J.Q., Tian Y., Xiong X.J. and Zheng T.L., Biological decolorization of the reactive dyes Reactive Black 5 by a novel isolated bacterial strain Enterobacter sp. EC3, J Hazard Mater,171(1-3), 654-659 (2009)
  18. Bafana A., Chakrabarti T., Muthal P. and Kanade G., Detoxification of benzidine-based azo dye by E. gallinarum: time-course study, Ecotoxicol Environ Saf,72(3), 960-964 (2009)
  19. Widhi Handayani V., Irene Meitiniarti and Timotius K.H., Decolorization of Acid Red 27 and Reactive Red 2 by Enterococcus faecalis under a batch system, World Journal of Microbiology & Biotechnology,23, 1239-1244 (2007)
  20. Moutaouakkil A., Zeroual Y., Dzayri F. Z., Talbi M., Lee K. and Blaghen M., Decolorization of azo dyes with Enterobacter agglomerans immobilized in different supports by using fluidized bed bioreactor, Curr Microbiol,48(2), 124-129 (2004)
  21. Waghmode T.R., Kurade M.B., Lade H.S. and Govindwar S.P., Decolorization and biodegradation of Rubine GFL by microbial consortium GG-BL in sequential aerobic/microaerophilic process, Appl Biochem Biotechnol,167(6), 1578-1594 (2012)
  22. Dawkar V.V., Jadhav U.U., Jadhav S.U. and Govindwar S.P., Biodegradation of disperse textile dye Brown 3REL by newly isolated Bacillus sp. VUS, J Appl Microbiol,105(1), 14-24 (2008)
  23. Bhatti H.N., Akram N. and Asgher M., Optimization of culture conditions for enhanced decolorization of cibacron red FN-2BL by Schizophyllum commune IBL-6. Appl Biochem Biotechnol,149(3), 255-264 (2008)
  24. Chen K.C., Wu J.Y., Liou D.J. and Hwang S.C., Decolorization of the textile dyes by newly isolated bacterial strains, J Biotechnol,101(1), 57-68 (2003)
  25. Jain K., Shah V., Chapla D. and Madamwar D., Decolorization and degradation of azo dye--Reactive Violet 5R by an acclimatized indigenous bacterial mixed cultures-SB4 isolated from anthropogenic dye contaminated soil, J Hazard Mater,213-214, 378-386 (2012)
  26. Kumar Praveen G.N. and Sumangala K. Bhat., Fungal Degradation of Azo dye- Red 3BN and Optimization of Physico-Chemical Parameters, ISCA Journal of Biological Sciences,1(2), 17-24 (2012)
  27. Praveen Kumar G.N. and Bhat S.K., Decolorization of Azo dye Red 3BN by Bacteria, International Research Journal of Biological Sciences,1(5), 46-52 (2012)