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Optimization and Characterization of Indigenous Microorganisms Isolated from Tannery Effluents in Nigeria

Author Affiliations

  • Department of Microbiology, Delta State University, Abraka, NIGERIA

Int. Res. J. Environment Sci., Volume 2, Issue (10), Pages 14-21, October,22 (2013)

Abstract

Samples of tannery effluents collected at the end of waste discharge pipes of tanneries in Kano, Nigeria were analysed to isolate, identify and characterize the indigenous bacteria and fungi capable of highly utilizing the tannery effluent as the sole carbon source. On the basis of morphological and biochemical characterization, the bacterial isolates identified were Klebsiella aerogenes, Pseudomonas aeruginosa, Escherichia coli, Bacillus subtilis and Proteus vulgaris. The fungal isolates were Penicillium chrysogenum and Aspergillus niger. All the isolates had proliferated growth on Tannery Wastewater Agar (TWA). The bacterial isolates had 75-100% occurrence in the effluent samples which the fungal isolates were found preent in all the samples. The bacterial isolates had their optimal growth at temperature of 37C, pH 7, 20% tannin concentration, 20% ((NHSO) concentration and 5% inorganic nitrogen source. The optimal growth for the fungal isolates was achieved at temperature of 28C, pH 4.5, inorganic and organic nitrogen concentrations of 20% and 15% respectively and at 20% tannin concentration. All the isolates grew better in the inorganic source of nitrogen ((NHSO) than in the organic source (meat extract). The results revealed that the isolates were able to utilize the tannin hence the ability to grow well at the high concentration. They can thus be recommended for use in the biodegradation of tannin which is one of the major toxic constituents of tannery effluent.

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