A concise review on source, production, purification and characterization of L-asparaginase and its application in food industries
- 1Department of FQA, College of FPTBE, Anand Agricultural University, Anand, Gujarat, India
- 2Department of Biotechnology, Veer Narmad South Gujarat University, Surat, Gujarat, India
Int. Res. J. Biological Sci., Volume 9, Issue (4), Pages 71-76, November,10 (2020)
L-asparaginase enzyme belongs to amidase group which carry out the deamination of L-asparagines to ammonia and aspartic acid. It has been using as a drug for the treatment of disorders like acute lymphoblastic leukemia. Another significant use of L-asparaginase enzyme is in the food manufacturing industry, where it reduces acrylamide formation in fried or baked foods while maintaining their sensory attributes like flavor, odor, taste, etc. The generation of the acrylamide during baking and frying relies on the Maillard reactions, within that acrylamide formation occur from the reaction between L-asparagine amino acid and reducing sugars during baking or frying condition. At the same time, this gives good sensory characteristics to the final product. The application of L-asparaginase can control the acrylamide production without affecting the sensory properties of baked or fried food. During processing a balance of contact time and reaction conditions is very much desired for enzyme effectiveness. Thus, this review represents production, purification, and application of L-asparaginase in the food manufacturing industry.
- Hendriksen, H. V., Kornbrust, B. A., Ostergaard, P. R. and Stringer, M. A. (2009)., Evaluating the Potential for Enzymatic Acrylamide Mitigation in a Range of Food Products Using an L-asparaginase from Aspergillus oryzae., Journal of Agricultural and Food Chemistry, 57(10), 4168-4176.
- Kwan, J. M., Fialho, A. M., Kundu, M., Thomas, J., Hong, C. S., Das Gupta, T. K. and Chakrabarty, A. M. (2009)., Bacterial Proteins as Potential Drugs in the Treatment of Leukemia., Leukemia Research, 33, 1392-1399.
- Joint FAO/WHO Expert Committee on Food Additives, (2006)., Evaluation of Certain Food Additives and Contaminants., Sixty-fourth Report of the Joint FAO/WHO Expert Committee on Food Additives. WHO Technical Report Series, No. 930.
- Zyzak, D. V., Sanders, R. A. and Stojanovic, M. (2003)., Acrylamide Formation Mechanism in Heated Foods., Journal of Agricultural and Food Chemistry, 51(16), 4782-4787.
- Krasotkina, J., Borisova, A. A., Gervaziev, Y. V. and Sokolov, N. N. (2004)., One-Step Purification and Kinetic Properties of the Recombinant L-asparaginase from Erwiniacarotovora., Biotechnology and Applied Biochemistry, 39, 215-221.
- Mahajan, R. V., Saran, S., Kameswaran, K., Kumar, V. and Saxena, R. K. (2012)., Efficient Production of L-asparaginase from Bacillus licheniformis with Low-Glutaminase Activity: Optimization, Scale-Up and Acrylamide Degradation Studies., Bioresource Technology, 125, 11-16.
- Iyer, P. and Singhal, R. S. (2008)., Production of Glutaminase (E.C.22.214.171.124) from Zygosaccharomycesrouxii: Statistical Optimization Using Response Surface Methodology., Bioresource Technology, 99, 4300-4307.
- Sathish, T. and Prakasham, R. S. (2009)., Enrichment of Glutaminase Production by Bacillus subtilisrsp-gluin Submerged Cultivation Based on Neural Network Genetic Algorithm Approach., Journal of Chemical Technology and Biotechnology, 85, 50-58.
- Qin, M. and Zhao, F. S. (2003)., L-asparaginase Release from Escherichia coli Cells with Aqueous Two-Phase Micellar Systems., Applied Biochemistry and Biotechnology, 110(1), 11-21.
- Campbell, H. A. and Mashburn, L. T. (1969)., L-asparaginase EC-2 from Escherichia coli. Some Substrate Specificity Characteristics., Biochemistry, 8, 3768-3775.
- Shrivastava, A., Khan, A., Shrivastav, A., Jain, K. and Singhal, K. (2012)., Kinetic Studies of L-asparaginase from Penicilliumdigitatum., Preparative Biochemistry and Biotechnology, 42(6), 574-581.
- Babu, K. R. and Satyanarayana, T. (1996)., Production of Bacterial Enzymes by Solid State Fermentation., Journal of Scientific and Industrial Research, 55, 464-467.
- Dias, F. F. G., de Castro, R. J. S., Ohara, A., Nishide, T. G., Bagagli, M. P. and Sato, H. H. (2015)., Simplex Centroid Mixture Design to Improve L-asparaginase Production in Solid-State Fermentation using Agroindustrial Wastes., Biocatalysis and Agricultural Biotechnology, 4(4), 528-534.
- Saqib, A. A., Hassan, M., Khan, N. F. and Baig, S. (2010)., Thermostability of Crude Endoglucanase from Aspergillus fumigatus Grown under Solid State Fermentation (SSF) and Submerged Fermentation (SmF)., Process Biochem, 45, 641-646.
- Cachumba, J. J. M., Antunes, F. A. F., Peres, G. F. D., Brumano, L. P., Santos, J. C. D. and Silva, S. S. D. (2016)., Current Applications and Different Approaches for Microbial L-asparaginase Production., Brazilian Journal of Microbiology, 47(1), 77-85.
- Kenari, S. L. D., Alemzadeh, I. and Maghsodi, V. (2011)., Production of L-asparaginase from Escherichia coli ATCC 11303: Optimization by Response Surface Methodology., Food and Bioproducts Processing, 89, 315-321.
- Arastoo, B. D. (2015)., Production & Characterization of L-asparaginase from Pseudomonas aeruginosa strain SN004: Production Optimization by Statistical Methods., Biocatalysis and Agricultural Biotechnology, 4(3), 388-397.
- Prakasham, R. S., Rao, C. S., Rao, R. S., Lakshmi, G. S. and Sarma, P. N. (2007)., L-asparaginase Production by Isolated Staphylococcus Sp. -6A: Design of Experiment Considering Interaction effect for Process Parameter Optimization., Journal of Applied Microbiology, 102(5), 1382-1391.
- El-Bessoumy, A., Sarhan, M. and Mansour, J. (2004)., Production, Isolation, and Purification of L-asparaginase from Pseudomonas aeruginosa 50071 using Solid-State Fermentation., International Journal of Biochemistry and Molecular Biology, 37(4), 387-393.
- Golunski, S., Astolfi, V., Carniel, N., Oliveira, D., Di Luccio, M., Mazutti, A. and Treichel, H. (2011)., Ethanol Precipitation and Ultrafiltration of Inulinases from Kluyveromycesmarxianus., Separation and Purification Technology, 78, 261-265.
- Qin, M. and Zhao, F. (2003)., L-asparaginase Release from Escherichia coli Cells with Aqueous Two-Phase Micellar Systems., Applied Biochemistry and Biotechnology, 110(1), 11-21.
- Lopes, A. M., Oliveira-Nascimento, L. and de Ribeiro, A. (2015)., Therapeutic L-asparaginase: Upstream, Downstream and Beyond., Critical Reviews in Biotechnology, 8551, 1-18.
- Dash, C., Mohapatra, S. B., & Maiti, P. K. (2016)., Optimization, purification, and characterization of L-asparaginase from Actinomycetales bacterium BkSoiiA., Preparative Biochemistry and Biotechnology, 46(1), 1-7.
- Avramis, V. I. (2012)., Asparaginases: biochemical pharmacology and modes of drug resistance., Anticancer research, 32(7), 2423-2437.
- Tareke, E., Rydberg, P., Karlsson, P., Eriksson, S. and Tornqvist, M. (2002)., Analysis of Acrylamide, A Carcinogen Formed in Heated Foodstuffs., Journal of Agricultural and Food Chemistry, 50(17), 4998-5006.
- Hogervorst, J., Schouten, E., Konings, E., Goldbohm, A. and Brandt, P. (2007)., A Prospective Study on Dietary Acrylamide Intake and the Risk of Endometrial, Ovarian and Cancer Breast., Cancer Epidemiology Biomarkers and Prevention, 16, 2304-2313.
- Stadler, R. H., Blank, I., Varga, N., Robert, F., Hau, J., Guy, P. A., Robert, M. C. and Riediker, S. (2002)., Acrylamide from Maillard Reaction Products., Nature, 419, 449-450.
- Parker, J. K., Balagiannis, D. P., Higley, J., Smith, G., Wedzicha, B. L. and Mottram, D. S. (2012)., Kinetic Model for the Formation of Acrylamide during the Finish-Frying of Commercial French Fries., Journal of Agricultural and Food Chemistry, 60(36), 9321-9331.
- Zamora, R. and Hidalgo, F. (2008)., Contribution of Lipid Oxidation Products to Acrylamide Formation in Model Systems., J. Agric. Food Chem., 56(15), 6075-6080.
- Hamzalioglu, A. and Gokmen, V. (2012)., Role of Bioactive Carbonyl Compounds on the Conversion of Asparagine into Acrylamide During Heating., European Food Research and Technology, 235(6), 1093-1099.
- Pedreschi, F., Kaack, K. and Granby, K. (2008)., The Effect of L-asparaginase on Acrylamide Formation in French Fries., Food Chemistry, 109, 386-392.
- Amrein, T. M., Schoonbachler, B., Escher, F. and Amado, R. (2004)., Acrylamide in Ginger Bread: Critical Factors for Formation and Possible Ways for Reduction., Journal of Agricultural and Food Chemistry, 52(13), 4282-4288.
- Hendriksen, H. V., Budolfsen, G. and Baumann, M. J. (2013). L-asparaginase for Acrylamide Mitigation in Food. Aspects of Applied Biology, 116, 41-50., undefined, undefined
- Vass, M., Amrein, T. M., Schonbachler, B., Esher, F., and Amadom R. (2004)., Ways to Reduce the Acrylamide Formation in Cracker Products., Czech Journal of Food Science, 22, 19-21.
- U.S. FDA (2016)., Guidance for Industry Acrylamide in Foods., U.S. Department of Health and Human Services Food and Drug Administration Center for Food Safety and Applied Nutrition. Accessed online at http://www.fda.gov/ FoodGuidances.