6th International Young Scientist Congress (IYSC-2021) and workshop on Intellectual Property Rights on 8th and 9th May 2021.  10th International Science Congress (ISC-2020) will be Postponed to 8th and 9th December 2021 Due to COVID-19.  International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Haematological alteration in fish Heteropneustes fossilis (Bloch) on exposure to fly ash

Author Affiliations

  • 1School of Life Sciences, Sambalpur University, Jyoti Vihar, Burla-768019, India
  • 2School of Life Sciences, Sambalpur University, Jyoti Vihar, Burla-768019, India
  • 3Dera Natung Government College, Itanagar, Arunachal Pradesh-791113, India

Int. Res. J. Biological Sci., Volume 9, Issue (2), Pages 47-50, May,10 (2020)


Study on the changes in haematological parameter of air breathing fish Heteropneustes fossilis was done on exposed to different concentration of fly ash. After estimating the LC50 of fly ash, fishes were exposed to different concentration of fly ash (200g/l, 400g/land 600 g/l) for a period of one week. The haematological parameters like haemoglobin (Hb), total erythrocyte count (TEC), packed cell volume (PCV) and total leucocyte count (TLC) were estimated. The absolute values like mean cell haemoglobin (MCH), mean cell haemoglobin concentration (MCHC) and mean cell volume (MCV) were calculated from the above parameters. There was significant alteration in all the haematological parameters on exposure to fly ash. Erythrocyte indices showed decrease from the control value whereas there was significant increase in total leucocyte count indicating stressful condition.


  1. Walia, Archana, and N. K. Mehra (1998)., A seasonal assessment of the impact of coal fly ash disposal on the river Yamuna, Delhi. I. Chemistry., Water, Air, and Soil Pollution, 103, 1-4, 277-314.
  2. Smolka-Danielowska, D. (2006), Heavy metals in fly ash from a coal fired power station in Poland., Polish J of Environ Stud, 15(6), 943-946.
  3. Sijakova-Ivanova, T., Z. Panov, K. B Lazev and V. Z. Paneva (2011)., Investigation of fly ash heavy metals content and physico chemical properties from thermal power plant, Republic of Macedonia., Int. J. of Eng. Sc. and Tech., 3(12), 8219-8225
  4. Pradhan, S., R. Behera and Prasanta Nanda (2019)., Study of fly ash toxicity to fish Heteropneustes fossilis (Bloch)., Int. Res. J. Biological. Sci., 8(8), 1-8.
  5. Nanda, P. (2014)., Bioaccumulation of heavy metals and physiological response in Anabas testudineus on exposure to paper mill effluent., J. Environ. Anal. Toxicol, 5(1), 1-8.
  6. Satish Akhila and R.K. Singh (2017)., Effect of Fly Ash on the haematological parameters of Channapunctatus (Bloch)., World Jour. Pharm. Pharmactl Sci; 6(7), 1309-1314.
  7. Dacie Lewis (1975):, Practical Haematology., 5th edition, Churchil-Livingston, Edinburg.
  8. Nanda P (1997):, Haematological changes in the common indian cat fish Heteropneustes fossilis under nickel stress., J. Ecobiol., 9(4), 243-246.
  9. Ali, M., Parvez, S., Pandey, S., Atif, F., Kaur, M., Rehman, H., & Raisuddin, S. (2004)., Fly ash leachate induces oxidative stress in freshwater fish Channa punctata (Bloch)., Environment International, 30(7), 933-938.
  10. Javed, M., & Usmani, N. (2012)., Toxic effects of heavy metals (Cu, Ni, Fe Co, Mn, Cr, Zn) to the haematology of Mastacembelus armatus thriving in Harduaganj Reservoir, Aligarh, India., Global Journal of Medical Research, 12(8), 59-64.
  11. Pallaniappan R. and Muthulingam M (2016)., Effect of heavy metal, Chromium on protein and aminoacid contents of gill, liver and kidney of freshwater fish Channastriatus (Bloch)., Intr. J. of Curr. Microbiol, and Appl. Sc., 5(7), 372-381.
  12. Kumari Archana, A. Srivastava and M. M. Jha (2014)., Carbaryl induced alterations in histology and certain biochemical parameters in liver of Clariasbatrachus., Global. Jour. Boisc. Boitech., 3(3), 259-263.
  13. Timothy W. Lohner, Robin J. Reash, V. Ellen Willet, Linda A.Rose (2001)., Assessment of Tolerant Sunfish Populations (Lepomis sp.) Inhabiting Selenium-Laden Coal Ash Effluents: 1. Hematological and Population Level Assessment., Elsevier; 50(3), 203-216.
  14. Sharma J. and S. Langer. (2014)., Effect of manganese on haematological parameters of fish Garragotylagotyla., J of Entom. and Zool. Study, 2(3), 77-81.
  15. Çoğun, H. Y., & Şahin, M. (2013)., The effect of lead and zeolite on hematological and some biochemical parameters in Nile fish (Oreochromis niloticus)., Current Progress Biological Research, 12, 277-286.
  16. Afshan Sehar, S. Ali, A. Ameen, U.S. Ameen, M. Farid, S.A. Bharwana, F. Hannan and R. Ahmad (2014)., Effect of different heavy metal pollution on fish., Res. J. Chem. Environ. Sci., 2(2), 35-40.
  17. Figueiredo-Fernandes Antonio, J.V. Ferreira-Cardoso,S. Garcia-Santos, S. M. Monteiro, J. Carrola, P. Matos and A. Fontainhas-Fernandes (2007)., Histopathological changes in liver and gill epithelium of Nile tilapia, Oreochromisniloticus, exposed to waterborne copper., Pesq. Vet. Bras., 27(3), 103-109.
  18. Gupta K.S., Langer J., Sharma and Sharma S. (2012)., Effects of different sublethal concentration on manganese on the levels of cortical in Garragotylagotyla., Int. J. of Sc. And Res. Pub., 2(10), 1-3.
  19. Deshmukh Manisha R., S.G. Chirde and Y.A. Gadhikar (2015)., Histological and Histochemical study on the stomach and instestine of catfish Heteropneustes fossilis (Bloch 1794)., Global. Jour. Biol Agri. Health. Sc., 4(1), 16-23.
  20. Hadi A.A. and S.F. Alwan (2012)., Histopathological changes in gills, liver and kidney of fresh water fish, Tilapia zillii, exposed to alumunium., Int J. of Pharm. & Life Sci, 3(11), 2071-2081.
  21. Ganeshwade R.M., S.S. Sathe and S.R. Sonawane (2013)., Dimethoate Induced Histological Changes in the Intestine of Fresh Water fish Puntiusticto (Ham)., TFR, 2(2), 2319-4758.
  22. Nanda, P and S, Panigrahi (2016)., Haematological alteration in air breathing fishes on exposure to paper mill effluent., Biolife, 4(4), 704-706.
  23. Diana Thelagathoti Conrad and C. Manjulatha (2016)., A Histological study of polypoidal stomach in Nemipterus japonicas off Visakhapatnam Coast., Ind. J. of Sci. and Tech., l9(9), 1-8.
  24. Das Suchismita and A. Gupta (2013)., Cadmium induced histopathological changes in the intestine of Indian flying barb, Esomusdanricus, Curr., World Environ., 8(2), 305-308.