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Studies on the effects of ethidium bromide and DMSO on cellulase producing isolated bacterial strain

Author Affiliations

  • 1ITM University Gwalior, Madhya Pradesh, India
  • 2R&D, MRD Life Sciences Pvt. Ltd., Lucknow, Uttar Pradesh, India
  • 3R&D, MRD Life Sciences Pvt. Ltd., Lucknow, Uttar Pradesh, India
  • 4Department of Chemistry, Sacred Heart Degree College, Sitapur, Uttar Pradesh, India

Int. Res. J. Biological Sci., Volume 9, Issue (1), Pages 7-16, February,10 (2020)

Abstract

Cellulase enzymes hydrolyse cellulose and are discharged by the microorganisms that grown over cellulose rich matter. Production of cellulase at marketing level is most actively grown research area now a day. Screening of potential strain from new source and there by optimizing production condition for industrial cellulase. Cellulase is one of the several profit making enzymes which have been serviced in different industries like paper and pulp, textile, production of bio-fuel, detergents, feed and food industry and brewing Bacterial cellulose possess more advantages when compared to the cellulose from other sources. In present study 14 Cellulase generating bacteria were deserted from wood degrading area, soil under dead leaves, dung of cow, and forest area. Purification and screening for cellulase production of isolates were done. Results indicate that bacteria SPCP1910 showed best result. Isolated strain was made better by treatment with DMSO and Ethidium Bromide.

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