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Phytochemical analysis, antioxidant, antimicrobial, and antiurolithiasis activities of endophytic Penicillium notatum isolated from Aloe vera roots

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Author Affiliations

  • 1Department of Microbiology and Biotechnology, Karnatak University, Dharwad, Karnataka, India and Department of Life Sciences, Mandsaur University, Mandsaur, M.P. India
  • 2Department of Microbiology, New arts, Commerce and Science College, Ahmednagar, Maharashtra, India
  • 3Department of Microbiology, New arts, Commerce and Science College, Ahmednagar, Maharashtra, India
  • 4Department of Microbiology, Dr. Babasaheb Ambedkar Marathawada University, Sub-Campus Osmanabad, Maharashtra, India
  • 5Department of Microbiology, S.B.B Alias Appasaheb Jedhe College of Arts, Commerce & Science, Pune, India
  • 6Department of Botany, Karnatak University, Dharwad, Karnataka

Int. Res. J. Biological Sci., Volume 8, Issue (10), Pages 31-37, October,10 (2019)

Abstract

The objective of present investigation was to assess phytochemical analysis, antioxidant, antimicrobial, and antiurolithiasis activities of endophytic fungi derived bioactive compounds obtained from Aloe vera roots. Fungal endophytes were isolated by means of potato dextrose agar media from Aloe vera roots and grown in Potato dextrose broth for the production of the bioactive compounds. The qualitative phytochemical screening was performed for the aqueous crude extract of the fungus. Phytochemicals like phenols and flavonoids were quantitatively estimated and further purified by thin layer chromatography and FTIR. The antiurolithiasis and antimicrobial activity of crude extracts was analyzed. A whole of six fungal endophytes were isolated and Penicillium notatum were identified as core endophytic fungus by 18S rRNA analysis. The fungus revealed total 1.86g and 0.15g of wet and dried biomass in PDB/100ml with 2.60g/100ml of the aqueous crude extract. The phytochemical screening showed terpenoids, flavonoids, saponins, phenols and tannins, alkaloid, cardiac glycosides, fixed oils and fats, proteins, carbohydrates with 2.26g of phenol and 0.22g of flavonoids/100g of gallic acid equivalent quantitatively. TLC revealed intermediate polar basic compounds, polar basic compounds, alkaloids, flavonoids, saponins, and terpenoids while FTIR analysis documented different functional groups such as alkanes, amides, alkenes, alcohol and phenols, alkyl halides, ether, amines, and nitrile groups. Ferric ions reducing power was reported at the rate of 1.2407±0.00702 while PM assays reported 0.8983±0.00351. The crude extract showed highest inhibition zone at the 100% concentration against Escherichia coli (16.3±0.57) and Aspergillus niger (20.5± 0.24). The maximum antiurolithiasis activity showed at 100% concentration at the rate of 67.5±1.49 of crude extract.

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