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Isolation of Indole acetic acid producing bacteria from digester effluent and their effect on plant growth promotion

Author Affiliations

  • 1Department of Microbiology, Lal Bahadur Shastri College of Arts, Science and Commerce, Satara-415002, MS, India

Int. Res. J. Biological Sci., Volume 8, Issue (1), Pages 1-9, January,10 (2019)

Abstract

The synthesis and release of indole acetic acid is an important property of bacteria that play key role in stimulating growth of crops. Indole acetic acid is phytohormone involved in growth and development in plants. The study aimed for isolation and to identify IAA producing bacteria from digester effluent of vegetable waste based biomethanation plant and to test its growth stimulatory effect on crops. Seventeen bacteria were isolated from digester effluent. They were tested to determine production ability for IAA. Two most potent IAA producing bacteria were selected further to test its stimulatory effect on growth of crops by pot assay method. The potent IAA producing plant growth promoting bacterial isolates were identified by molecular characterization using 16 S rRNA gene analyses. The results obtained from pot experiment demonstrated that two most potent IAA producing bacterial isolates cause significant increase in plant development parameters of inoculated crop plants by comparing with control. The study suggests two potent IAA producing bacteria from digester effluent can serve as efficient biofertilizer inoculants to enhance soil fertility and plant growth promotion.

References

  1. Matsukawa E., Nakagawa Y., Iimura Y. and Hayakawa M. (2007)., Stimulatory effect of indole-3- acetic acid on aerial mycelium formation and antibiotic production in Streptomyces spp., Actinomycetologica, 21, 32-39.
  2. Carreno-Lopez R., Campos-Reales N., Elmerich C. and Baca B.E. (2000)., Physiological evidence for differently regulated tryptophan-dependent pathways for indole-3-acetic acid synthesis in Azospirillum brasilense., Mol. Gen. Genet., 264(4), 521-530.
  3. Spaepen S., Vanderleyden J. and Remans R. (2007)., Indole-3-acetic acid in microbial and microorganism-plant signaling., FEMS Microbiol. Rev., 31(4), 425-448.
  4. Persello-Cartieaux F., Nussaume L. and Robaglia C. (2003)., Tales from the underground: molecular plant-rhizobacteria interactions., Plant Cell Environ., 26(2), 189-199.
  5. Zhao Y. (2010)., Auxin biosynthesis and its role in plant development., Annu. Rev. Plant Biol., 61, 49-64.
  6. Teale W.D., Paponov I.A. and Palme K. (2006)., Auxin in action: signaling, transport and the control of plant growth and development., Nat. Rev. Mol. Cell Biol., 7(11), 847- 859.
  7. Swain M.R., Naskar S.K. and Ray R.C. (2007)., Indole 3-acetic acid production and effect on sprouting of yam, (Dioscorea rotundata L) Minisetts by Bacillus subtilis isolated from culturable cowdung microflora., Pol. J. Microbiol., 56(2), 103-110.
  8. Pattern C.L. and Glick B.R. (2002)., Role of Pseudomanas putida indo lactic acid in development of the host plant root system., Appl. Environ. Microbiol., 68(8), 3795-3801.
  9. Harikrishnan H., Shanmugaiah V. and Balasubramanian N. (2014)., Optimization for production of Indole acetic acid (IAA) by plant growth promoting Streptomyces sp VSMGT1014 isolated from rice rhizosphere., Int. J. Curr. Microbiol. App. Sci., 3(8), 158-171.
  10. Pant G. and Agrawal P.K. (2014)., Isolation and characterization of indole acetic acid producing plant growth promoting rhizobacteria from rhizospheric soil of Withania somnifera., Journal of Biological and Scientific Opinion, 2(6), 377-383.
  11. Owamah H.I., Dahunusi S.O., Oranusi U.S. and Alfa M.I. (2014)., Fertilizer and sanitary quality of digestate biofertilizer from co-digestion of food waste and human excreta., Waste manag., 34(4), 747-752.
  12. Hassan D.U. and Abdulsalam S. (2017)., Assessement of bio-fertilizer quality of anaerobic digestion of watermelon peels and cow dung., Chemical and Biomolecular Engineering, 2(3), 135-141.
  13. Alfa M.I., Adie D.B., Igboro S.B., Oranusi U.S., Dahunsi S.O. and Akali D.M. (2014)., Assessment of biofertilizer quality and health implications of anaerobic digestion effluent of cow dung and chicken droppings., Renewable Energy, 63, 681-686.
  14. Report (2008)., Bio-fertilizer Entrepreneurial training manual., Tamil Nadu Agricultural University, Coimbatore, India.
  15. Rahman A., Sitepu I.R., Tang S.Y. and Hashidoko Y. (2010)., Salkowski's reagent test as a primary screening index for functionalities of Rhizobacteria isolated from wild dipterocarp saplings growing naturally on medium-strongly acidic tropical peat soil., Biosci. Biotechnol. Biochem., 74(11), 2202-2208.
  16. Gordon S.A. and Weber R.P. (1951)., Colorimetric estimation of indoleacetic acid., Plant Physiol., 26(1), 192-195.
  17. Chung K.R., Shilts T., Erturk U., Timmer L.W. and Uenq P.P. (2003)., Indole derivatives produced by the fungus Colletotrichum acutatum causing lime anthracnose and postbloom fruit drop of citrus., FEMS Microbiol. Lett., 226(1), 23-30.
  18. Sujatha N. and Ammani K. (2013)., Siderphore production by the isolates of fluorescent pseudomonads., Int. J. Cur. Res. Rev., 5(20), 1-7.
  19. Cappuccino J. and Sherman N. (2010)., Microbiology: A Laboratory Manual., 9th edition, Benjamin Cummings Publishing Company, USA.
  20. Alstrom S. and Burns R.G. (1989)., Cyanide production by rhizobacteria as a possible mechanism of plant growth inhibition., Biol. Fertil. Soils, 7(3), 232-238.
  21. Vos P., Garrity G., Jones D., Krieg N.R., Ludwig W., Rainey F.A., Schleifer K.H. and Whitman W.B. (2009)., Bergey, The Firmicutes, 2nd Edition, Springer Dordrecht Heidelberg London, New York, 3, 1-1422.
  22. Brenner D.J., Krieg N.R. and Staley J.T. (2005)., Bergey, Part C, Second Edition, Springer, 2, 308-316.
  23. Wahyudi A.T., Astuti R.P., Widyawati A., Meryandini A. and Nawangsih A.A. (2011)., Characterization of Bacillus sp. strains isolated from rhizosphere of soybean plants for their use as potential plant growth for promoting Rhizobacteria., J.Microbiol. Antimicrob., 3(2), 34-40.
  24. Sridevi M. and Mallaiah K.V. (2007)., Bioproduction of indole acetic acid by rhizobium strains isolated from root nodules of green manure crop., Sesbania sesban (L.) Merr. Iranian J. Biotech., 5(3), 178-182.
  25. Lwin K.M., Myint M.M., Tar T. and Aung W.Z.M. (2012)., Isolation of plant hormone (indole-3-acetic acid-IAA) producing rhizobacteria and study on their effects on maize seedling., Engineering Journal, 16(5), 137-144.
  26. Ahmad F., Ahmad I. and Khan M.S. (2005)., Indole Acetic Acid production by the indigenous isolates of Azotobacter and fluorescent Pseudomonas in the presence and absence of Tryptophan., Turk J. Biol., 29, 29-34.
  27. Malik D.K. and Sindhu S.S. (2011)., Production of indole acetic acid by Pseudomonas sp.: effect of coinoculation with Mesorhizobium sp. Cicer on nodulation and plant growth of chickpea (Cicer arietinum)., Physiol. Mol. Biol. Plants, 17(1), 25-32.
  28. Fatima Z., Saleemi M., Zia M., Sultan T., Aslam M., Rehman R. and Chaudhary M.F. (2009)., Antifungal activity of plant growth promoting rhizobacteria isolates against Rhizoctonia solani in wheat., Afr. J. Biotechnol., 8(2), 219-225.
  29. Xu Z., Shao J., Li B., Yan X., Shen Q. and Zhang R. (2013)., Contribution of Bacillomycin D in Bacillus amyloliquefaciens SQR9 to antifungal activity and biofilm formation., Appl. Environ. Microbiol., 79(3), 808- 815.
  30. Pidello A. (2003)., The effect of Pseudomonas fluorescens strains varying in psoverdine production on the soil redox status., Plant and soil, 253(2), 373-379.