5th International Young Scientist Congress (IYSC-2019).  International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Transcriptomic analysis of Agelenopsis naevia (Aranae: Agelenidae) Venom Gland

Author Affiliations

  • 1Department of Zoology, Ahmadu Bello University Zaria, Nigeria
  • 2Department of Zoology, Ahmadu Bello University Zaria, Nigeria
  • 3Department of Zoology, Ahmadu Bello University Zaria, Nigeria

Int. Res. J. Biological Sci., Volume 7, Issue (3), Pages 9-23, March,10 (2018)

Abstract

The venom of spider is made up of toxins with varying biological activities whose selectivity and affinity for various receptors and ion channels are yet to be sufficiently explored and exploited. Recently, transcriptomics have been employed as a tool to reveal the molecular diversity and structure of animal venoms across species. Thus, this study was carried out to determine the transcripts coding for toxins in the venom gland of Agelenopsis naevia, collected in open gardens of Ahmadu Bello University Zaria, Nigeria. Venom glands were isolated using micro dissection followed by mRNA extraction. A cDNA library was constructed and pair-end sequencing was carried out. A total of 11,167,123 reads were generated which were assembled into 33,182 sequences. Fourty eight (48) transcript coded for proteins/peptides amongst which are sphingomyelinase-D, hyaluronidase, astacin-like metalloproteases, techylectin and cystine knot toxins. The results provide insight towards the discovery of novel potential bioinsecticides and/or drug leads from Agelenopsis Naevia venom for agro-allied/pharmaceutical applications.

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