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Pseudozyma flocculosa Y-1: A potent hydrolytic yeast isolate from the biogas digester effluent

Author Affiliations

  • 1Department of Microbiology, Lal Bahadur Shastri College of Arts, Science and Commerce, Satara-415002, MS, India
  • 2Department of Microbiology Yashavantrao Chavan Institute of Science, Satara-415002, MS, India

Int. Res. J. Biological Sci., Volume 7, Issue (10), Pages 6-9, October,10 (2018)

Abstract

Vegetable waste serves as source of nuisance in markets. The current unscientific disposal methods lead to environmental pollution. Biomethanation is a promising biological treatment method for vegetable wastes. Biomethanation process converts organic matter into biogas and manure. The different types of microorganisms are involved in production of biogas. Hydrolysis is the first step in biomethanation and different kinds of aerobic and anaerobic microorganisms are involved in the process. The biomethanation experiment was carried out in 5litre biogas digester under ambient temperature conditions. The yeasts were isolated by using Sabourauds agar medium. The yeast isolates were subjected to determine their ability for the production of hydrolytic enzymes. The yeast isolate with maximum hydrolytic potential was identified by morphological, cultural, biochemical characterization and molecular identification by 24 S rRNA.

References

  1. Kumar S., Bhattacharyya J.K., Vaidya A.N., Chakrabarti T., Devotta S. and Akolkar A.B. (2009)., Assessment of the status of municipal solid waste management in metro cities, state capitals, class I cities, and class II towns in India: An insight., Waste management, 29(2), 883-895.
  2. Charles W., Walker L. and Cord-Ruwisch R. (2009)., Effect of pre-aeration and inoculum on the start-up of batch thermophilic anaerobic digestion of municipal solid waste., Bioresource technology, 100(8), 2329-2335.
  3. Christy P.M., Gopinath L.R. and Divya D. (2014)., A review on anaerobic decomposition and enhancement of biogas production through enzymes and microorganisms., Renewable and Sustainable Energy Reviews, 34, 167-173.
  4. Weiland P. (2010)., Biogas production: current state and perspectives., Applied microbiology and biotechnology, 85(4), 849-860.
  5. Zverlov V.V., Hiegl W., Kock D.E., Kellermann J., Kollmeier T. and Schwarz W.H. (2010)., Hydrolytic bacteria in mesophilic and thermophilic degradation of plant biomass., Eng. Life Sci., 10(6), 528-536.
  6. Zverlov V.V. and Schwarz W.H. (2008)., Bacterial cellulose hydrolysis in anaerobic environmental subsystems-Clostridium thermocellum and Clostridium stercorarium, thermophilic plant fibre degraders, in Incredible Anaerobes, (Ed: J. Wiegel)., Annals N.Y. Acad. Sci., 1125, 298-307.
  7. Kazda M., Langer S. and Bengelsdorf F.R. (2014)., Fungi open new possibilities for anaerobic fermentation of organic residues., Energy, Sustainability and Society, 4(6), 1-9.
  8. Deshmukh H.V. (2013)., Facultative and non methanogenic micro flora from biogas digester runs on distillery waste and Ipomoea weed biomass., Trends in Biotechnology Research, 2(1), 1-3.
  9. Bengelsdorf F.R. (2012)., Characterization of the microbial community in a biogas reactor supplied with organic residues., Ph.D Dissertation, Faculty of Natural Sciences, University of Ulm.
  10. Santiago A.L.C.M.D. and Motta C.M.D.S. (2008)., Isolation of Mucorales from processed maize (Zea mays L.) and screening for protease activity., Brazilian Journal of Microbiology, 39(4), 698-700.
  11. Lu W.J., Wang H.T., Nie Y.F., Wang Z.C., Huang D.Y., Qiu X.Y. and Chen J.C. (2004)., Effect of inoculating flower stalks and vegetable waste with ligno-cellulolytic microorganisms on the composting process., Journal of Environmental Science and Health, Part B, 39(5-6), 871-887.
  12. Bandounas L., Nick J.P., Wierck W.J.H. and Ruijssenaars H.J. (2011)., Isolation and characterization of novel bacterial strain exhibiting ligninolytic potential., BMC Biotechnol., 11(94), 1-11.
  13. Hendricks C.W., Doyle J.D. and Hugley B. (1995)., A new solid medium for enumerating cellulose-utilizing bacteria in soil., Appl. Environ. Microbiol., 61(5), 2016-2019.
  14. Kurtzman C.P. and Fell J.W. (1998)., The Yeasts, A taxonomic study (4th ed)., Elsevier Press, Amsterdam.
  15. Barnett J.A., Payne R.W. and Yarrow D. (1990)., Descriptions of the species arranged alphabetically., Yeasts: characteristics and identification, 2nd ed. Cambridge University Press, Cambridge, United Kingdom, 679-695.
  16. Campbell J. and Duffus J.H. (1988)., Yeast: A Practical Approach., IRL Press, Oxford.
  17. Gibbs B.M. and Shapton D.A. (1968)., Identification Methods for Microbiologists., Academic Press, New York.
  18. Kreger van Rij N.J.W. (1984)., The Yeasts. A taxonomic study., Amsterdam: Elsevier Science Publishers B.V.
  19. Rose A.H. and Harrison J.S. (1969)., The Yeasts, Biology of Yeasts., Academic Press, London, 1.
  20. Belanger R.R., Labbe C. and Jarvis W.R. (1994)., Commercial-scale control of rose powdery mildew with a fungal antagonist., Plant Dis., 78, 420-424.
  21. Hajlaoui M.R. and Bélanger R.R. (1993)., Antagonism of the yeast‐like phylloplane fungus Sporothrix flocculosa against Erysiphe graminis var tritici., Biocontrol Science and technology, 3(4), 427-434.
  22. Jarvis W.R., Shaw L.A. and Traquair J.A. (1989)., Factors affecting antagonism of cucumber powdery mildew by Stephanoascus flocculosus and S. rugulosus., Mycol. Res., 92, 162-165.
  23. Mimee B., Labbé C. and Bélanger R.R. (2009)., Catabolism of flocculosin, an antimicrobial metabolite produced by Pseudozyma flocculosa., Glycobiology, 19(9), 995-1001.
  24. Mimee B., Labbé C., Pelletier R. and Bélanger R.R. (2005)., Antifungal activity of flocculosin, a novel glycolipid isolated from Pseudozyma flocculosa., Antimicrobial agents and chemotherapy, 49(4), 1597-1599.