MicroRNAs Specific Primer Design using miRNA Design Tool

Author Affiliations

  • 1Veer Narmad South Gujarat University, Surat, Gujarat, India
  • 2Veer Narmad South Gujarat University, Surat, Gujarat, India

Int. Res. J. Biological Sci., Volume 6, Issue (8), Pages 44-46, August,10 (2017)

Abstract

MicroRNAs (miRNAs) are tiny (only 18-24 nucleotide long) non-coding RNAs which involved in post-transcriptional regulation of gene expression in multi-cellular organisms by affecting both translation of mRNAs and the stability. There are several different methods used for quantification of miRNAs like Northern Blotting, quantitative RT-PCR, Microarray etc. but, quantitative RT-PCR is used as the standard that is used to validate & confirmation of the results of various methods. The design of primers for miRNA qRT PCR is very much difficult because of short length of miRNA, which size is the very much near to the length of normal PCR primers. The miRNA Design Tool is based on the Universal Probe Library (ULP) probes to design primer(s) for miRNA detection. The tool is a software based and easy method for design of working primers for target specific miRNA for qRT-PCR. The application is available as online service by AstridBio.

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