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Removal of lead and inhibition of algal growth using prodigiosin produced by serratia marcescens

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Author Affiliations

  • 1Department of Biological Sciences, Niger State Polytechnic Zungeru, Niger State, Nigeria
  • 2Department of Microbiology, Federal University of Technology, Minna, Nigeria
  • 3Department of Microbiology, Federal University of Technology, Minna, Nigeria
  • 4Department of Microbiology, Kaduna State University, Kaduna State, Nigeria
  • 5Department of Microbiology, Federal University of Technology, Minna, Nigeria
  • 6Department of Microbiology, Federal University of Technology, Minna, Nigeria

Int. Res. J. Biological Sci., Volume 6, Issue (8), Pages 1-6, August,10 (2017)

Abstract

This study examined the removal of lead and inhibition of Anabaena sphaerica and Oscillatoria agardhii growth using prodigiosin produced by Serratia marcescens. Inhibition of the algal growth was studied by the addition of different concentration of prodigiosin 50µl, 100µl, 150µl in 90ml of algal culture. Control was without the pigment prodigiosin. Inhibition rates were determined at the interval of 72 hours of incubation using spectrophotometer. A.sphaerica record highest level of inhibition in all concentrations. It was observed to record highest levels of inhibition at 100µg/L concentration of prodigiosin which was 76.7%, while O.agardhii was 66.3% at the same concentration. At concentration of 50µg/L A.sphaerica record of 66.3% inhibition with O.agardhii recording 64.3% while at concentration of 150µg/L it was 67.2% and 66.5% respectively. Statically analysis shows no significant differences between the rate of inhibition by the two algal species studied but there was significant difference between concentration of prodigiosin and the rate of inhibition. Removal of lead polluted soil sample was studied by the addition of different concentration of prodigiosin 50µl, 100µl, 150µl to 5g of lead polluted soil in 90ml of distilled water. The lead removal rate was determined at the interval of 4 weeks of incubation for six months using atomic absorption spectroscopy (AAS). The result shows that 100µl of prodigiosin enhance high amount of lead removal from soil. The amount of lead immobilized by the pigment at 20 weeks was 52.5% and become stable after 24 weeks. This was followed by 50µL and 150µL of prodigiosin in which there was immobilization of 41.2% and 35.3% respectively at 20 weeks of treatment.The results suggest that the red pigment inhibited cyanobacteria growth and can be used as a potential for the removal of soil polluted with lead.

References

  1. White J., Bibb M. and Bld A. (2007)., Dependence of undecyl prodigiosin production in Streptomyces coelicolor A3 (2) involves a pathway-specific regulatory cascade., J. Bacteriology, 179, 627-633.
  2. Ajayi S.O. and Osibanjo O. (1981)., Pollution studies on Nigerian rivers. 2. Water quality of some Nigerian rivers., Environmental Pollution, 2(2), 87-95.
  3. Kadiri M.O. (2006)., Phytoplankton flora and physicochemical attributes of some water in Eastern Nigeria., Nigerian Journal of Botany, 19(2), 188-200.
  4. Khanafari Anita, Assadi Mazaheri M. and Fakhr Ahmadi F. (2006)., Review of prodigiosin, Pigmentation in Serratia marcescens., Journal of Biological Sciences, 6(1), 1-13.
  5. Adesalu T.A. and Nwankwo D.I. (2005)., Studies on the phytoplankton of Olero creek and parts of Benin River, Nigeria., Ekologia, 3(2), 21-30.
  6. Jeong H., Yim J.H., Lee C., Choi S.H., Park Y.K., Yoon S.H., Hur Cheol-Goo, Kang Ho-Young, Kim Dockyu, Hee Lee Hyun, Hyang Park Kyun, Park Seung-Hwan, Park Hong-Seog, Kum Lee Hong, Kwang Oh Tae and Kim Jihyun F. (2005)., Genomic blueprint of Hahella chejuensis, a marine microbe producing an algicidal agent., Nucleic Acids Research, 33(22), 7066-7073.
  7. Kim D., Lee J.S., Park Y.K., Kim J.F., Jeong H., Oh T.K., Kim B.S. and Lee C.H. (2007)., Biosynthesis of antibiotic prodiginines in the marine bacterium Hahella chejuensis KCTC 2396., Applied Microbiology, 102(4), 937-944.
  8. Pandey S., Sree A., Dash S.S., Sethi D.P. and Chowdhury L. (2013)., Diversity of marine bacteria producing beta-glucosidase inhibitors., Microbiology of Cell Fact, 12, 35.
  9. Tsuda K., Takamura N., Matsuyama M. and Fujii Y. (2011)., Assessment method for leaf litters allelopathic effect on cyanobacteria., Journal of Aquatic and Plant Management, 43, 43-46.
  10. Anderson D.M. (2009)., Approaches to monitoring, control and management of harmful algal blooms (HABs)., Ocean Coastal Management, 52(7), 342-347.
  11. Sengco M.R. and Anderson D.M. (2004)., Controlling harmful algal blooms through clay flocculation., Journal of Eukaryotic Microbiology, 51(2), 169-172.
  12. Van Hullebusch E., Deluchat V., Chazal P.M. and Baudu M. (2002)., Environmental impact of two successive chemical treatments in a small shallow eutrophied lake: Part II. Case of copper sulfate., Environmental Pollution, 120(3), 627-634.
  13. Alam Z.B., Otaki M., Furumai H. and Ohgaki S. (2001)., Direct and indirect inactivation of Microcystis aeruginosa by UV-radiation., Water Research, 35(4), 1008-1014.
  14. Su J., Yang X., Zhou Y. and Zheng T. (2011)., Marine bacteria antagonistic to the harmful algal bloom species Alexandrium tamarense (Dinophyceae)., Biological Control, 56, 132-138.
  15. Jeong S.Y., Ishida K., Ito Y., Okada S. and Murakami M. (2003)., Bacillamide, a novel algicide from the marine bacterium, Bacillus sp. SY-1, against the harmful dinoflagellate, Cochlodinium polykrikoides., Tetrahedr. Letters, 44(43), 8005-8007.
  16. Bai S.J., Hung L.P., Sue J.Q., Tien Y. and Zeng T.L. (2011)., Algicide effect of a novel marine actinomycete on the toxic dinoflagellate Alexandrium tamarense., Current Microbiology, 62, 1774-1781.
  17. Cai W., Wang H., Tien H., Tian Y., Chen F. and Zheng T.L. (2011)., Influence of a bacteriophage on the population dynamic of toxic dinoflagellate by lysis of algicidal bacteria., Applied and Environmental Microbiology, 77(21), 7837-7840.
  18. Mayali X. and Azam F. (2004)., Algicidal bacteria in the sea and their impact on algal blooms., Journal of Eukaryotic Microbiology, 51(2), 139-144.
  19. Wang X., Gong L., Liang S., Han X., Zhu C. and Li Y. (2012)., Algicidal activity of rhamnolipid biosurfactants produced by Pseudomonas aeruginosa., Harmful Algae, 4(2), 433-443.
  20. Yoshinaga I., Kim M.C., Katanozaka N., Imai I., Uchida A. and Ishida Y. (1998)., Population structure of algicidal marine bacteria targeting the red tide forming alga Heterosigma akashiwo (Raphidophyceae), determined by restriction fragment length polymorphism analysis of the bacterial 16S ribosomal RNA genes., Marine Ecology, 170, 33-44.
  21. Wang X., Gong L., liang S., Han X.J., Zhu C. and Li Y. (2005)., Algicidal activity of rhamnolipid biosurfactants by Pseudomona aeruginosa., Harmful Algae, 4(2), 433-443.
  22. Imai I., Fujimaru D., Nishigaki T., Kurosaki M. and Sugita H. (2006)., Algicidal bacteria isolated from the surface of seaweeds from the coast of Osaka Bay in the Seto Inland Sea, Japan., African Journal of Marine Sciences, 28(2), 319-323.
  23. Lee S.O., Kato J., Takiguchi N., Kuroda A., Ikeda T., Mitsutani A. and Ohtake H. (2000)., Involvement of an extracellular protease in algicidal activity of the marine bacterium Pseudoalteromonas sp. strain A28., Applied Environmental Microbiology, 66(10), 4334-4339.
  24. Cho J.C. and Giovannoni S.J. (2004)., Cultivation and growth characteristics of a diverse group of oligotrophic marine Gammaproteobacteria., Applied Environmental Microbiology, 70(1), 432-440.
  25. Nagayama K., Shibata T., Funjimoto K., Honjo T. and Nakamur T. (2003)., Algicidal effect of phlorotannins from the brown algae Eckloniakurome on red tide microalgae., Aquaculture, 218(1-4), 601-611.
  26. Jin Z.P., Luo K., Zhang S., Zheng Q. and Yang H. (2012)., Bioaccumulation and catabolism of prometryne in green algae., Chemosphere, 87(3), 278-284.
  27. Furstner A. (2003)., Chemistry and biology of roseophilin and the prodigiosin alkaloids: a survey of the last 2500 years., Angew. Chemistry International Edition England, 42(31), 3582-3603.
  28. Takamatsu S., Hodges T.W., Rajbhandari I., Gerwick W.H., Hamann M.T. and Nagle D. G. (2003)., Marine natural products as novel antioxidant prototypes., Journal of Natural Products, 66(5), 605-608.
  29. Dembitsky V.M., Rezanka T., Spiìzek J. and Hanus L.O. (2005)., Secondary metabolites of slime molds (Myxomycetes)., Phytochemistry, 66, 747-769.
  30. Mekhael H. and Yousif H. (2009)., The role of red pigment produced by Serratia marcescens as antibacterial plasmid curring agent., Journal of Duhok University, 12(1), 268-274.
  31. Berlanaga M., Ruiz N., Hernandez-Borrell J., Montero T. and Vinas M. (2000)., Role of outer membrane in the accumulation of quinolones by Serratia marcescens., Canadian Journal of Microbiology, 46, 716-721.