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In vitro bioactivity and phytochemical characterization of Nypa fruticans. Wurmb: a mangrove from Kerala, India

Author Affiliations

  • 1Department of Botany, St’ Teresa’s College, Ernakulam, Kerala, India
  • 2Department of Botany, St’ Teresa’s College, Ernakulam, Kerala, India

Int. Res. J. Biological Sci., Volume 6, Issue (6), Pages 41-51, June,10 (2017)

Abstract

Nypa fruticans Wurmb is an underutilized mangrove plant in India. Mangroves generally contain more secondary metabolites to thrive in a saline environment. The present study focuses on estimation of primary metabolites like carbohydrates, proteins, lipids etc and secondary metabolites like flavonoids, phenols, tannins etc from different parts of Nypa plant such as leaf, stem, male flower, bracts, unripe endosperm and ripe endosperm. Total phenolic content (79.91±1.63 mg/g) and flavonoid contents (99.5±0.936mg/g) were high in the leaves compared to young and mature endosperm. The antioxidant activity using DPPH and SOD scavenging assay also showed very high in leaves. IC50 value of leaves for DPPH scavenging assay was 6.11±1.68 µg/ml and SOD scavenging assay was 11.85±1.16 µg/ml. GC-MS and LC-MS analysis of NFME of leaves identified several bioactive compounds. Brenzcatechin, 4-vinylphenol, 4-vinyl 2-methoxy-phenol, hexadecanoic acid etc were some of the compounds obtained from GC-MS analysis. In vitro study of methanolic extract for anti-diabetic, anti-arthritic, and anti-inflammatory activity were evaluated and % inhibition was high. Percentage of &

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