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Combined effects of metals and chlorophenols on dehydrogenase activity of bacterial consortium

Author Affiliations

  • 1Department of Microbiology, Federal University of Technology, P.M.B. 1526, Owerri, Nigeria
  • 2Department of Microbiology, Federal University of Technology, P.M.B. 1526, Owerri, Nigeria
  • 3Department of Microbiology, Federal University of Technology, P.M.B. 1526, Owerri, Nigeria
  • 4Department of Microbiology, Federal University of Technology, P.M.B. 1526, Owerri, Nigeria

Int. Res. J. Biological Sci., Volume 6, Issue (4), Pages 10-20, April,10 (2017)


Toxicity of Zinc, Cadmium, 4-Chlorophenol (4-CP), 2,4-Dichlorophenol (2,4-DCP) and their binary and quaternary mixtures were determined based on inhibition of dehydrogenase activity of a consortium of Pseudomonas, Bacillus, Micrococcus and Staphylococcus species. The toxicity of chemicals and their mixtures were evaluated in the concentration range of 0-3mM while Cadmium and 2,4-Dichlorophenol binary mixture range was 0-1.8mM. Zinc, 4-CP and 2,4-DCP exhibited hormetic effect at low concentration. The IC50 were determined using monotonic and hormesis dose-response models. The binary and quaternary mixtures of the pollutants evaluated showed progressive inhibition of the enzyme activity. The combined effects of the mixtures on the enzyme activity of the bacterial consortium were evaluated with isobolographic representation and toxic index (TI) model. The isobolographic analysis indicated additive, synergistic and antagonistic interactions for the various binary mixtures evaluated. However, the TI of most mixtures was within the range of 0.5-2.0 and are considered additive. Modulation of the toxic interactions by the components of the mixture through synergistic and antagonistic interaction of the heavy metals and phenolic compounds against the dehydrogenase activity of the bacterial consortium were possible depending on the relative amount of the components.


  1. Gikas P. (2007)., Kinetic responses of activated sludge to individual and joint nickel (Ni(II)) and cobalt (Co(II)): An isobolographic approach., Journal of Hazardous Materials, 143(1), 246-256. http:/dx.doi.org/10.1016/j.jhazmat. 2006.09.019
  2. Nies D.H. (1999)., Microbial heavy metal resistance., Applied Microbiology and Biotechnology, 51(6), 730-750.
  3. Șengor S.S., Barua S., Gikas P., Ginn T.R., Peyton B., Sani R.K. and Spycher N.F. (2009)., Influence of heavy metals on microbial growth kinectics including Lag Time: Mathematical modeling and experimental verification., Environmental Toxicology and Chemistry, 28(10), 2020-2029. http:/dx.doi.org/10.1897/08-273.1
  4. Babich H. and Stotzky G. (1982)., Nickel toxicity to microbes: Effect of pH and implications for acid rain., Environmental Research, 29(2), 335-350.
  5. Mowat A. (1976)., Measurement of metal toxicity by biochemical oxygen demand., Journal of the Water Pollution Control Federation, 48(5), 853-866.
  6. Sandrin T. and Maier R. (2003)., Impact of metals on the biodegradation of organic pollutants., Environmental Health Perspective, 111(8), 1093-1101. http:/dx.doi.org/ 10.1289/ehp.5840
  7. Gikas P. and Romanos P. (2006)., Effects of tri-valent (Cr (III)) and hexavalent (Cr(VI)) chromium on the growth rate of activated sludge., Journal of Hazardous Materials, 133(1), 212-217. http:/dx.doi.org/10.1016/j.jhazmat.2005.10.023
  8. Nwanyanwu C.E., Nweke C.O., Orji J.C. and Opurum C.C. (2013)., Phenol and heavy metal tolerance among petroleum refinery effluent bacteria., Journal of Research in Biology, 3, 922-931.
  9. Stohs S.J. and Bagchi D. (1995)., Oxidative mechanisms in the toxicity of metal ions., Free Radical Biology and Medicine, 18(2), 321-336.
  10. Peitzsch N., Eberz G. and Nies D.H. (1998)., Alcaligenes eutrophus as a bacterial chromate sensor., Applied and Environmental Microbiology, 64(2), 453-458.
  11. Roane T.M., Rensing C., Pepper I.L. and Maier R.M. (2008)., Microorganisms and Metal Pollution., Environmental microbiology, 2nd Elsevier Science, San Diego, CA., 427-448.
  12. Nweke C.O. and Okpokwasili G.C. (2010)., Influence of exposure time on phenol toxicity to refinery wastewater bacteria., Journal of Environmental Chemistry and Ecotoxicology, 2(2), 20-27.
  13. Kibret M., Somitsch W. and Robra K.H. (2000)., Characterization of a phenol degrading mixed population by enzyme assay., Water Research, 34(4), 1127-1134. http:/dx.doiorg/10.1016/S0043-1354(99)00248-1
  14. Song-Hu Y. and Xiao-Hua L. (2005)., Comparison treatment of various chlorophenols by electro-Fenton method: Relationship between chlorine content and degradation., Journal of Hazardous Materials, 118(1), 85-92. http:/dx.doi.org/10.1016/j.jhazmat.2004.08.025
  15. Manojlovic D., Ostojic D.R., Obradovic B.M., Kuraica M.M., Krsmanovic V.D. and Puric J. (2007)., Removal of phenol and chlorophenols from water by new ozone generator., Desalination and Water Treatment, 213(1-3), 116-122. http:/dx.doi.org/10.1016/j.desal.2006.05.059
  16. Nweke C.O. and Okpokwasili G.C. (2013)., Removal of phenol from aqueous solution by adsorption onto activated carbon and fungal biomass., International Journal of Biosciences, 3(8), 11-21. http://dx.doi.org/ 10.12692/ijb/3.8.11-21
  17. Keweloh H., Weyrauch G. and Rehm H.J. (1990)., Phenol induced membrane changes in free and immobilized Escherichia coli., Applied Microbiology and Biotechnology, 33(1), 66-71. http:/dx.doi.org/10.1007/ BF00170572.
  18. Choi S.H. and Gu M.B. (2001)., Phenolic toxicity: detection and classification through the use of a recombinant bioluminescent Escherichia coli., Environmental Toxicology and Chemistry, 20(2), 248-255.
  19. Santos V.L., Heilbuth N.M. and Linardi V.R. (2001)., Degradation of phenol by Trichosporon sp. LE3 cells immobilized in alginate., Journal of Basic Microbiology, 41(3-4), 171-178.
  20. Quilchano C. and Maranon T. (2002)., Dehydrogenase activity in Mediterranean forest soil., Biology and Fertility of Soils, 35(2), 102-107. http:/dx.doi.org/10.1007/s00374-002-0446-8
  21. Nweke C.O. and Okpokwasili G.C. (2010)., Inhibition of dehydrogenase activity in petroleum refinery wastewater bacteria by phenolic compounds., Ambi-Água, 5(1), 6-16. http:/dx.doi.org/10.4136/ambi-agua.115
  22. Preston S., Coad N., Townend J., Killham K. and Paton G.I. (2000)., Biosensing the acute toxicity of metal interactions: are they additive, synergistic, or antagonistic?., Environmental Toxicology and Chemistry, 19(3), 775-780.
  23. Le-Blanc G.A. and Wang G.R. (2006)., Chemical mixtures greater than additive effects., Environmental Health Perspective, 114(9), 517-518.
  24. Choi S.H. and Gu M.B. (2002)., A portable toxicity biosensor using freeze-dried recombinant bioluminescent bacteria., Biosensors and Bioelectronics, 17(5), 433-440.
  25. Nweke C.O., Ahumibe N.C. and Orji J.C. (2014)., Toxicity of binary mixtures of formulated glyphosate and phenols to Rhizobium Species dehydrogenase activity., Journal of Microbiology Research, 4(4), 161-169. http:/dx.doi.org/ 10.5923/j.als.20150502.01
  26. Schabenberger O., Tharp B.E., Kells J.J. and Penner D. (1999)., Statistical test for hormesis and effective dosages in herbicide dose–response., Agronomy Journal, 91(4), 713-721. http:/dx.doi.org/10.2134/agronj1999.914713x
  27. Boillot C. and Perrodin Y. (2008)., Joint-action ecotoxicity of binary mixtures of glutaraldehyde and surfactants used in hospitals: use of the Toxicity Index model and isobologram representation., Ecotoxicology and Environmental Safety, 71(1), 252-259. http:/dx.doi.org/10.1016/j.ecoenv. 2007.08.010
  28. Calabrese E.J. and Blain R. (2005)., The occurrence of hormetic dose responses in the toxicological literature, the hormesis database: an overview., Toxicology and Applied Pharmacology, 202(3), 289-301.
  29. Christofi N., Hoffmann C. and Tosh L. (2002)., Hormesis responses of free and immobilized light-emitting bacteria., Ecotoxicology and Environmental Safety, 52(3), 227-231. http:/dx.doi.org/10.1006/eesa.2002.2203
  30. Mahgoub S., Abdelbasit H. and Abdelfattah H. (2015)., Removal of phenol and zinc by Candida isolated from wastewater for integrated biological treatment., Desalination and Water Treatment, 53(12), 3381-3387.
  31. Altenburger R., Nendza M. and Schüürmann G. (2003)., Mixture toxicity and its modeling by quantitative structure–activity relationships., Environmental Toxicology and Chemistry, 22(8), 1900-1915.
  32. Deneer J.W. (2000)., Toxicity of mixtures of pesticides in aquatic systems., Pest Management Science, 56(6), 516-520.
  33. Brzo´ska M.M., Galaz´yn-Sidorczuk M., Rogalska J., Roszczenko A., Jurczuk M., Majewska K. and Moniuszko-Jakoniuk J. (2008)., Beneficial effect of zinc supplementation on biomechanical properties of femoral distal end and femoral diaphysis of male rats chronically exposed to cadmium., Chemical and Biological Interactions, 171(3), 312-324.
  34. Xu X., Li Y., Wang Y. and Wang Y. (2011)., Assessment of toxic interactions of heavy metals in multi-component mixtures using sea urchin embryo-larval bioassay., Toxicology in Vitro, 25(1), 294-300. http:/dx.doi.org/10.1016/j.tiv.2010.09.007
  35. Zhu B.Z., Shechtman S. and Chevion M. (2001)., Synergistic cytotoxicity between pentachlorophenol and copper in a bacterial model., Chemosphere, 45(4), 463-470.
  36. Ishaque A.B., Johnson L., Gerald T., Boucaud D., Okoh J. and Tchounwou P.B. (2006)., Assessment of individual and combined toxicities of four non-essential metals (As, Cd, Hg and Pb) in the Microtox Assay., International Journal of Environmental Research and Public Health, 3(1), 118-120.
  37. Mowat F.S. and Bundy K.J. (2002)., Experimental and mathematical/computational assessment of the acute toxicity of chemical mixtures from the Microtox® assay., Advances in Environmental Research, 6(4), 547-558. http:/dx.doi.org/10.1016/S1093-0191(01)00099-5
  38. Heipieper H.J., Diefenbach R. and Keweloh H. (1992)., Conversion of cis unsaturated fatty acids to trans: a possible mechanism for the protection of phenol-degrading Pseudomonas putidaP8 from substrate toxicity., Applied and Environmental Microbiology, 58(6), 1847-1852.
  39. Okolo J.C., Nweke C.O., Nwabueze R.N., Dike C.U. and Nwanyanwu C.E. (2007)., Toxicity of phenolic compounds to oxidoreductases of Acinetobacter species isolated from a tropical soil., Scientific Research EssayS, 2(7), 244-250.
  40. Ren S. and Frymier P.D. (2002)., Estimating the toxicities of organic chemicals to bioluminescent bacteria and activated sludge., Water Research, 36(17), 4406-4414. http:/dx.doi.org/10.1016/j.watres.2010.01.009
  41. Rajani M.R., Sreekanth D. and Himabindu V. (2011)., Degradation of mixture of phenolic compounds by activated sludge processes using mixed consortia., International Journal of Energy and Environment, 2(1), 151-160.
  42. Agarry S.E., Solomon B.O. and Durojaiye A.O. (2008)., Microbial degradation of phenols: a review., International Journal of Environmental and Pollution, 32(1), 12-28.
  43. Beelen P.V. and Fleuren-Kemila A.K. (1997)., Influence of pH on the toxic effects of Zinc, Cadmium, and Pentachlorophenol on pure cultures of soil microorganisms., Environmental Toxicology and Chemistry, 16(2), 146-153. http:/dx.doi.org/ 10.1002/ etc.5620160208