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Screening of protease produced by bacterial isolates of bakery industry

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Author Affiliations

  • 1Department of Microbiology, Cashew Export promotion Council of India (CEPCI), Mundakkal, Kollam, Kerala, India
  • 2Department of Microbiology, Cashew Export promotion Council of India (CEPCI), Mundakkal, Kollam, Kerala, India

Int. Res. J. Biological Sci., Volume 6, Issue (2), Pages 36-41, February,10 (2017)

Abstract

Quantitative estimation of protease produced by bacterial isolates of bakery industry was performed. Primary screening of protease production was carried out using gelatine agar plates followed by quantitative estimation of protease was conducted for five bacterial isolates named as TKMFT8,TKMFT22,TKMFT25, TKMFT39 and TKMFT53 using MRS broth inoculated with incubated over a 10 days period in a rotary shaker at 37&

References

  1. Theron L.W. and Divol B. (2014)., Microbial aspartic proteases: current and potential applications in industry., Appl Microbiol Biotechnol , 98(21), 8853-8868.
  2. Verma O.P, Prashansa K., Shruti S. and Abha S. (2011)., Production of Alkaline Protease by Bacillus subtilis (MTCC7312) using Submerged Fermentation and Optimization of Process Parameters., European Journal of Experimental Biology, 1, 124-129.
  3. Sumantha A., Larroche C. and Pandey A. (2006)., Microbiology and industrial biotechnology of food-grade proteases: a perspective., Food Technol Biotechnol, 44(2), 211-220.
  4. Ward O.P., Rao M.B. and Kulkarni A. (2009)., Proteases, Production., Encyclopedia of Enzymes, Schaechter, M., Ed., New York: Elsevier Inc., 495-511.
  5. Vasantha S.T. and Subramanian A.T. (2012)., Optimization of cultural conditions for the production of an extracellular protease by Pseudomonas species., International Current Pharmaceutical Journal, 2(1), 1-6.
  6. Anita A. and Rabeeth M. (2010)., Isolation, purification and characterization of protease enzyme from Bacillus sp., possible use in biodiesel production., Asian Jr of Microbiolo Biotech Env Csi, 12, 71-77.
  7. Ganzle M.G., Loponen J. and Gobbetti M. (2008)., Proteolysis in sourdough fermentations: mechanisms and potential for improved bread quality., Trends in Food Science & Technology, 19(10), 513-521.
  8. Rao M.B., Tanksale A.M., Ghatge M.S. and Deshpande V.V. (1998)., Molecular and biotechnological aspects of microbial proteases., Microbiology and Molecular Biology Reviews, 62(3), 597-635.
  9. Barrett A.J. (1995)., Proteolytic enzymes: aspartic and metallopeptidases., Methods Enzymol , 248, 183-228.
  10. Tiwari O.N., Devi T.B., Devi K.S., Oinam G., Indrama T., Ojit K., Avijeet O. and Ningshen L. (2015)., Isolation and optimization of alkaline protease producing Bacteria from undisturbed soil of NE-region of India falling under Indo-Burma biodiversity hotspots., Journal of Applied Biology & Biotechnology, 3(4), 25-31.
  11. Sankeerthana C., Pinjara S., Jambagia R.T., Bhavimania S., Anupama S., Sarovar B. and Inamdar S.R. (2013)., Production and Partial Characterization of Protease from Aspergillus flavus using Rice Mill Waste as a Substrate and its Comparison with Aspergillus Niger Protease., International Journal of Current Engineering and Technology, 1, 143-147.
  12. Sjodahl J., Emmer A., Vincent J. and Roeraade J. (2002)., Characterization of proteinases from Antarctic krill (Euphausia superba)., Protein Expression and Purification, 26(1), 153-161.
  13. Tsuchida O., Yamagata Y., Ishizuka T., Arai J., Yamada J., Takeuchi M. and Ichishima E. (1986)., An alkaline protease of an alkalophilic Bacillus sp., Curr. Microbiol, 14(1), 7-12.
  14. Lowry O.H., Rosebrough N.J., Farr A.L. and Randall R.J. (1951)., Protein measurement with Folin phenol reagent., J. Biol. Chem, 193(1), 265-275.
  15. Folasade M. and Joshua O. (2005)., Production dynamics of extracellular protease from bacillus species., African J. Biotech, 4(8), 776-779.
  16. Cupp-Enyard C. (2008)., Sigma’s non-specific protease activity assay Casein as a substrate., J. Vis. Exp, 19, 899.
  17. Khan M.A., Nadeem Ahmad, Zafar A.U., Nasir I.A. and Qadir M.A. (2011)., Isolation and screening of alkaline protease producing bacteria and physio-chemical characterization of the enzyme., African Journal of Biotechnology, 10(33), 6203-6212.
  18. Sharma A.K., Sharma V., Saxena J., Yadav B., Alam A. and Prakash A. (2015)., Isolation and Screening of Extracellular Protease Enzyme from Bacterial and Fungal Isolates of Soil., International Journal of Scientific Research in Environmental Sciences, 3(9), 334-340.
  19. Alnahdi H.S. (2012)., Isolation and screening of extracellular proteases produced by new isolated Bacillus sp., J. Appl. Pharma. Sci, 2(9), 71-74.
  20. Sinha P., Singh R.K., Srivastva R., Sharma R. and Tiwari S.P. (2013)., Characterization and optimization of alkaline protease enzyme produced by soil brone bacteria., Trends Life Sci, 2(2), 38-46.
  21. Smita G.S., Ray P. and Mohapatra S. (2012)., Quantification and Optimisation of Bacterial Isolates for Production of Alkaline Protease., Asian J. Exp. Biol. Sci., 3, 180-186.