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Use of leaves of mulberry, Morus alba (L) treated with Stevia Inulin for the improvement of activities of enzymes in the mid gut protease and amylase of the last stage silkworm larvae

Author Affiliations

  • 1Department of Zoology, Shardabai Pawar Mahila Mahavidyalaya Shardanagar, Tal-Baramati, Dist-Pune, Pin-413 115, India
  • 2Department of Zoology, Shardabai Pawar Mahila Mahavidyalaya Shardanagar, Tal-Baramati, Dist-Pune, Pin-413 115, India

Int. Res. J. Biological Sci., Volume 6, Issue (11), Pages 24-30, November,10 (2017)


The inulin is a heterogeneous collection of fructose polymers anda soluble dietary fiber. The present attempt is concerned with use of leaves of mulberry, Morus alba (L) treated with Stevia Inulin for the improvement of activities of mid gut protease and amylase in last stage silkworm larvae. Four different concentrations of aqueous solution of herbal formulation: Stevia inulin powder (5.0 mg/ lit; 10.0 mg/ lit; 20.0 mg/ lit and 50.0 mg/ lit) were used to soak the leaves of mulberry. The treated leaves were used feeding. The schedule of feeding for each strength of solution for the first group (A-First; B-First; C-First and D-First) was for the three days (First; Second and Third); For second group (A-Second; B-Second; C-Second and D-Second)) was for two days (second and third) and for the third group (A-Third; B-Third; C-Third and D-Third) was only for one day (third). The analysis of biochemical parameters was carried through the use of fifth stage instars of five days old. The biochemical characters considered for analysis were proteins (Soluble fraction of Proteins and Total fraction of Proteins). The activities of enzymes, namely protein digesting enzyme (protease) and carbohydrate digesting enzyme (amylase) in were also considered in the present attempt. The tissue used for the biochemical analysis was mid gut homogenate. Soaking the mulberry leaves with Stevia inulin powder and supplying the treated leaves to last larval instar was found effected into significant changes contents of both soluble and total proteins. The activities of protein digesting enzyme (protease) and carbohydrate digesting enzyme (amylase) were also found effected through the inulin treatment. The contents of soluble proteins and total proteins of mid gut homogenate pattern was found increased respectively from 32.147 percent to 90.074 percent and 5.657 percent to 39.052 percent. The activities of protein digesting enzyme (protease) and carbohydrate digesting enzyme (amylase) were observed to increase from 21.444 to 83.706 and from 14.540 to 54.257 percent respectively. The composition of Stevia inulin powder serve to fortify the digestion. It may also exert the effects on activity in metabolism in the last stage larval of silkworm, Bombyx mori (L). Inclusion of the method of “Treating the leaves of mulberry with Stevia inulin powder before the feeding” may be effecting to speed up the rate of metabolism in the last larval instars of silkworm. Utilization of Stevia inulin powder for soaking the leaves of mulberry before supplying to the last larval stage of silkworm, Bombyx mori (L) may be considered for introduction in the “Methodlogy of Sericulture. It may help to improve the health of silkworm.


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