9th International Science Congress (ISC-2019).  International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Heterochromatin Distribution in the species of Iphegenia Kunth

Author Affiliations

  • 1Department of Botany, Hutatma Rajaguru Mahavidyalaya, Rajagurunagar, MS, India

Int. Res. J. Biological Sci., Volume 6, Issue (1), Pages 40-43, January,10 (2017)


Banding patterns were revealed in the somatic chromosome of 4 species Iphigenia Kunth using HCl-Giemsa staining techniques. There was considerable variation both regarding the amount and distribution of bands; C-banding of chromosome promises to be the most valuable technique for routine chromosome analysis due to its inherent simplicity, sensitivity and stability of the material obtained. HCl- Giemsa banding technique was used to study Heterochromatin (HC) banding pattern in genus Iphigenia. The genus Iphigenia is a monocot. The species studied here named as, I. pallida (Baker), I stellata (Blat), I. magnifica (A and R) and I. indica (Linn.) were characterized by 2n= 22 chromosome. The chromosome complement exhibited telomeric and centromeric HC. I. magnifica (A and R) and I. indica (Linn.) showed more banded chromosomes than I. pallida (Baker) and I. stellata (Blat). Telomeric bands were mostly present on short arm of the chromosomes.


  1. Unakul W. and Hsut C. (1972)., The C-and G-banding patterns of Rattus noryegicus Chromosomes., J Nat Cancer Inst, 49, 1425-1431
  2. Yosida T.H. and Sagai T. (1972)., Banding pattern analysis of polymorphic Karyotype in black art by a new differential staining technique., Chromosoma, 37, 387-394.
  3. Evans H.J., Buckland R.A. and Sumner A.T. (1973)., Chromosome homology and heterochromatin in goat, sheep ox studied by banding techniques., Chromosoma, 42, 387-403.
  4. Natarajan A.T. and Sharma N.P. (1974)., Chromosome banding patterns and the origin of the B genome in wheat (T.aestivum var.lokwan)., Genet Res, 24, 103-108.
  5. De Vries J.M. and Sybenga J. (1976)., Identification of Rye-Chromosomes: The giemsa banding pattern and the translocation tester set., Theo and Appl Genet, 35-43.
  6. Islam A.K.M.R. (1980)., Identification of wheat barley addition lines with N-banding of chromosomes., Chromosoma, 76, 365-373.
  7. Arrighi F.E., Hsu T.C., Saunders F. and Saunders G.F. (1971)., Localization of repetitive DNA in the chromosome of Mierotya agrestris by means of in situ hybridization., Chromosoma, 32, 224-236.
  8. Vosa C.G. (1976)., Heterochromatin banding patterns in Allium II Heterochromatin variation in species of the Paniculatum group., Chromosoma, 57, 119-133.
  9. Mizianty M. (1984a)., Banding patterns in plant chromosomes., II. Bibliography (1970-1980) - Anthophyta. Acta Soc. Bot. Pol. 53 (Suppl. 1), 485-487.
  10. Mizianty M. (1984b)., Banding patterns in plant chromosomes., II. Bibliography (1970-1980) - Anthophyta. Acta Soc. Bot. Pol. 53,111-136.
  11. John B. (1988)., The biology of heterochromatin., In: Heterochromatin Molecular and Structural Aspects (Verma, R.S., ed.). Cambridge University Press, Cambridge, 1-47.
  12. Hennig W. (1999)., Heterochromatin., Chromosoma, 108,1-9.
  13. Joshi and Ranjekar (1983)., Mechanism of Hcl (hydrochloric acid) Giemsa Banding Technique., The Neucleus. 26(1), 35- 48.
  14. C.P. Joshi and P.K. Ranjekar (1980)., Technique for heterochromatin visualization and chromosome banding in plants., Nucleus 23, 169.
  15. Vosa C.G. (1985)., Chromosome banding in plants., In: Chromosome and Cell Genetics (Sharma, A.K. and Sharma, A., eds.). Gordon and Breach Science Publishers, London, 79-104.
  16. Coming D.E. and Harris D.C. (1976)., Nuclear proteins., II Exp. Cell Res. 96:161.
  17. Sumner A.T. (1990)., Chromosome Banding., Unwin Hyman, London.
  18. Filion W.G. (1974)., Giemsa staining in plant., I Banding patterns three cultivars of Tulipa Chromosoma. 49, 51-60.
  19. Benget B. and Landstrom T. (1975)., Polymorphism in chromosome of Leopoldia comosa (Liliaceae) revealed by Giemsa staining., Hereditas, 80, 219-232.
  20. Lavania U.C. and Sharma A.K. (1979)., Trypsinorcin banding in plant chromosomes., Stain Tech., 54, 261.
  21. Blaky D.H. and Vosa C.G. (1982)., Heterochromatin and chromosome variation in cultivated species of Tulipa subg Leiostemones., Pl Syst Evol., 138, 163-178.
  22. Delay C. (1947)., Recherches sur la structure des noyaux quiescents chez les phanérogames., Ver. Cytol. Cytophysiol. Veg. 10, 103-228.
  23. Barlow P. (1977)., Determinants of nuclear chromatin structure in angiosperms., Ann. Sci. Nat. Bot. Biol. Veg. 18, 193-206.
  24. Guerra M. (1987)., Cytogenetics of Rutaceae IV. Structure and systematic significance of interphase nuclei., Cytologia. 52, 213-222.