9th International Science Congress (ISC-2019).  International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Synthesize a transfer vector containing the gene encoding ADP-glucose pyrophosphorylase enzyme to improve the starch content in Manihot esculenta Crantz

Author Affiliations

  • 1Faculty of Biology - Chemistry, Tay Bac University, Quyet Tam, District - Sơn La City, Vietnam
  • 2Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Vietnam
  • 3Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Vietnam

Int. Res. J. Biological Sci., Volume 6, Issue (1), Pages 25-33, January,10 (2017)


Starch is polysaccharide which plays important role in the metabolism of living organisms. In addition, starch is raw materials in the food and materials industry. Increasing the starch content of crops based on improving the activity of some genes encoding enzymes plays an important role in the process of biosynthesis and accumulation of starch, which is one of the research are of interest. Like in bacteria, the process of starch biosynthesis in plants occurs by using ADP-Glc as raw materials to made long link chain &


  1. Thro A.M. (1996)., Cassava R&D: a public investment., Nat Biotechnol., 14(8), 929.
  2. K. Sriroth, K. Piyachomkwan, S. Wanlapatit (2000)., Cassava starch technology: the Thai experience., Starch‐Stärke, 52(12), 439-449.
  3. Fernie A.R., Willmitzer L. and Trethewey R.N. (2002)., Sucrose to starch: a transition in molecular plant physiology., Trends in plant science, 7(1), 35-41.
  4. Zeeman S.C., Kossmann J. and Smith A.M. (2010)., Starch: its metabolism, evolution, and biotechnological modification in plants., Annual review of plant biology, 61, 209-234.
  5. Tiessen A. et. al. (2002)., Starch synthesis in potato tubers is regulated by post-translational redox modification of ADP-glucose pyrophosphorylase a novel regulatory mechanism linking starch synthesis to the sucrose supply., The Plant Cell., 14(9), 2191-2213.
  6. U. Ihemere, D. Arias‐Garzon (2006)., Genetic modification of cassava for enhanced starch production., Plant Biotechnology Journal, 4(4), 453-465.
  7. Stark D.M. et. al. (1992)., Regulation of the amount of starch in plant tissues by ADP glucose pyrophosphorylase., Science, 258(5080), 287-292.
  8. S. Ball, T. Marianne, L. Dirick, M. Fresnoy, B. Delrue (1991)., A Chlamydomonas reinhardtii low-starch mutant is defective for 3-phosphoglycerate activation and orthophosphate inhibition of ADP-glucose pyrophosphorylase., Planta, 185(1), 17-26.
  9. Smidansky E.D. et. al. (2002)., Enhanced ADP-glucose pyrophosphorylase activity in wheat endosperm increases seed yield., Proceedings of the National Academy of Sciences, 99(3), 1724-1729.
  10. Sakulsingharoj C. et. al. (2004)., Engineering starch biosynthesis for increasing rice seed weight: the role of the cytoplasmic ADP-glucose pyrophosphorylase., Plant science, 167(6), 1323-1333.
  11. Merrick M.J., Gibbins J.R. and Postgate J.R. (1987)., A rapid and efficient method for plasmid transformation of Klebsiella pneumoniae and Escherichia coli., Microbiology, 133(8), 2053-2057.
  12. Sambrook J.G. et. al. (2002)., Fugu orthologues of human major histocompatibility complex genes: a genome survey., Immunogenetics, 54(6), 367-380.
  13. Ball S.G. and Morell M.K. (2003)., From bacterial glycogen to starch: understanding the biogenesis of the plant starch granule., Annual review of plant biology, 54(1), 207-233.
  14. Ballicora M.A., Iglesias A.A. and Preiss J. (2003)., ADP-glucose pyrophosphorylase, a regulatory enzyme for bacterial glycogen synthesis., Microbiology and Molecular Biology Reviews, 67(2), 213-225.
  15. Morán-Zorzano M.T. et. al. (2007)., Escherichia coli AspP activity is enhanced by macromolecular crowding and by both glucose‐1, 6‐bisphosphate and nucleotide‐sugars., FEBS letters, 581(5), 1035-1040.