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Quantification of LUPEOL in Excoecaria agallocha Leaf, Stem and Root by HPTLC

Author Affiliations

  • 1School of Environment and Sustainable Development, Central University of Gujarat, Gandhinagar 382030, Gandhinagar, Gujarat, India
  • 2School of Environment and Sustainable Development, Central University of Gujarat, Gandhinagar 382030, Gandhinagar, Gujarat, India

Int. Res. J. Biological Sci., Volume 6, Issue (1), Pages 1-5, January,10 (2017)


An attempt to augment to quantify Lupeol, pharmacologically bioactive triterpenoid in the Excoecaria Agallocha mangrove (methanol extracts of leaf, stem, root parts), has been made. A HPTLC method was developed with the combination of n- Hexane: Ethyl acetate: Methanol (9:1:1 v/v) as solvent system and TLC silica gel 60 F254plates as stationary phase under constant room temperature and relative humidity of 26.1 0.4 0C and 76 2 % respectively. Detection and quantification were performed by densitometry scanning at 540 nm after derivatization with Anisaldehide Sulfuric Acid Reagent (ASR). This method gave compact spots at RF 0.31 corresponding to Lupeol. As a result, the content of Lupeol in the leaf, stem and root were found 492.42 ng, 704.23 ng and 728.15 ng respectively as well as % amount (w/w) of Lupeol in the leaf, stem and root were found 0.09948, 0.14085 and 0.14568 respectively.


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