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Influence of Cellular Phone and Tower on Mitosis in Allium Cepa Model

Author Affiliations

  • 1Cell Biology, School of studies in Zoology and Biotechnology, Vikram University, Ujjain, MP, 456010, India
  • 2Cell Biology, School of studies in Zoology and Biotechnology, Vikram University, Ujjain, MP, 456010, India
  • 3Cell Biology, School of studies in Zoology and Biotechnology, Vikram University, Ujjain, MP, 456010, India
  • 4Cell Biology, School of studies in Zoology and Biotechnology, Vikram University, Ujjain, MP, 456010, India
  • 5Limnology Laboratory, School of studies in Zoology and Biotechnology, Vikram University, Ujjain, MP, 456010, India
  • 6Limnology Laboratory, School of studies in Zoology and Biotechnology, Vikram University, Ujjain, MP, 456010, India

Int. Res. J. Biological Sci., Volume 5, Issue (8), Pages 53-57, August,10 (2016)

Abstract

An attempt is made to know cytological effects of a cell phone (mobile phone) and a cell phone tower on mitotically dividing root tip cells in Allium cepa protocol. Unexposed Allium cepa bulbs grown in the laboratory acted as control (Gr I). Second group of bulbs were exposed overnight to a cell phone (CP) in a room on the first floor of building (Gr II). In third group bulbs were placed on the open terrace, outside the room and exposed to cell phone tower (CPT) located on the roof of same room (Gr III). Medium for growing Allium cepa for roots was laboratory tap water. At the end of experimentations (96 hours) dissolved oxygen (DO) was measured in the medium of all groups. Every subsequent day temperature of the medium was recorded for Gr I and II. Morphology of root tips was observed, mean root length (MRL) was calculated. N-HCl acetocarmine squash preparations were made to observe mitosis, chromosomal aberration, micronuclei formation and disturbed mitosis. Mitotic index was also calculated. Results revealed no change in the temperature of the medium due to cell phone exposure. Dissolved oxygen increased following both exposures; more in tower exposure Gr III. No morphological changes could be detected in Gr I and II but in Gr III i.e tower exposure caused ‘blunt ended’ tips which is an unusual observation. No aberrations, no micronuclei and no abnormal mitoses were seen in any group however, mean root length and mitotic index declined in Gr II and III; more in Gr III. Unusual mitosis could be seen in Gr III as most of the cells were seen arrested in ‘prophase’ only; no other stages seen. At some places prophase nuclei were seen fused. It is concluded that CP and CPT lowers mitosis but CPT affects more severely. Genotoxicity not detected.

References

  1. Jain A. and Bagai D. (2014)., Effects of exposure to electromagnetic radiations on living beings and environment., International Journal of Electrical, Electronics and Data Communication., 2(7), 1-5.
  2. Fathi E. and Farahzadi R. (2014)., Interaction of mobile telephone radiation with biological systems in veterinary and medicine., Journal of Biomedical Engineering & Technology., 2(1), 1-4.
  3. Gautam D. N. (2015)., Biological effects of electromagnetic waves from cell phones and cell phone towers: A Mini Review., Submitted as a part of M. Phil. Course work to Vikram University, Ujjain, India
  4. Ruediger H. W. (2009)., Genotoxic effects of radiofrequency electromagnetic fields., Pathophysiology., 16, 89.
  5. Fiskesjo G. (1995)., Allium Test., Methods in Molecular Biology,43(14), In Vitro Toxicity Testing Protocols (Edited by O’ Hare S, Atterwill C.K.). Humana Press Inc. Totowa, NJ., 119-127.
  6. APHA. (1998)., Standard methods for examinations of water and waste water., 20th edn., Washington DC.
  7. Winkler L.W. (1888)., Die Bestimmung des in Wasser gelösten Sauerstoffen., Berichte der Deutschen Chemischen Gesellschaft, 21, 2843-2855.
  8. Tkalec M., Malaric K., Pavalika M., Pevalek-Kozina B. and Vidakovic-Cifrek Z. (2009)., Effects of radiofrequency electromagnetic fields on seed germination and root meristematic cells of Allium cepa L., Mutation Research/Genetic Toxicity and Environmental Mutagenesis., 672(2), 76.
  9. Sharma V. P., Singh H. P., Kohli R. K. and Batish D. R. (2009)., Mobile phone radiation inhibits Vigna radiata (Mung bean) root growth by inducing oxidative stress., Sci Total Environ., 407(21), 5543-07.
  10. Akbal A., Kiran Y., Sachin A., Turgut-Balik D. and Balik H. H. (2012)., Effects of electromagnetic waves emitted by mobile phones on germination, root growth and root tip cell mitotic division of Lens Culinaris Medik., Pol. J. Environ. Stud., 21(1), 23-29.
  11. Fiskesjo G. (1993)., The Allium Test- A potential standard for the assessment of environmental toxicology., Environmental Toxicology and Risk Assessment, 2. ASTM STP 1216, Gorsuch JW, Dawyer FJ, Ingersoll CG, La Pount T W Eds, American Society for testing and Materials, Philadelphia, PA, 331-344.
  12. Pesnya D. S. and Romanovsky A. V. (2013)., Comparision of cytotoxic and genotoxic effects of plutonium-239 alpha particles and mobile phones GSM 900 radiation in the Allium cepa test., Mutation Research/Genetic Toxicity and Environmental Mutagenesis., 750(1-2), 27-33.
  13. Rathore H. S., Rathore M., Panchal S., Makwana M., Sharma A. and Shrivastava S. (2010)., Can genotoxic effect be model dependent in Allium test?- An Evidence., Environment Asia., 3(2), 29-33.
  14. Rathore H. S., Khare A., Sharma A., Shrivastava S. and Bhatnagar D. (2006)., A study on the cytological effects of myrobalan (Fruit of Terminalia chebula) in Allium tests., Ethanobotanical Leaflets., 10, 92-97.
  15. Marmat S. and Rathore H. S. (2013)., Cytological effects of Triphala in Allium cepa models., International Journal of Pharmacy & Technology., 5(2), 5602-5609.
  16. Kashiwada Y., Nonaka G., Nishioka I., Chang J. J. and Lee K. H. (1992)., Antitumor agents, 129, Tannins and related compounds as selective cytotoxic agents., J. Nat Prod., 55, 1033.
  17. Lee S.H., Ryn S. Y., Choi S. U., Lee C. O., No Z., Kim S. K. and Ahn J. W. (1995)., Hydrolysable tannins and related compounds having cytotoxic activity from fruit of Terminalia chebula., Arch. of Pharmacol Res., 18, 118.
  18. Kaur S., Micheal H., Arora S., Harbonen P. L. and Kumar S. (2005)., The in- vitro cytotoxic and apoptotic activity of Triphala: an Indian drug., J Ethnopharmacol., 97, 15-20.
  19. Jose J. K., Kuttan G. and Kuttan R. (2001)., Antitumor activity of Emblica officinalis., J Ethnopharmacol., 75, 65-69.
  20. Solanke P., Singh M., Rathore H. S., Sharma A., Makwana M. and Shrivastava S. (2007)., An evaluation of the genotoxic effects of seed decoction of Cassia tora Linn (Leguminosae) in Allium cepa model., Ethanobotanical Leaflets., 11, 217-223.
  21. Yamabhai M., Chumseng S., Yoohat K. and Srila W. (2014)., Diverse biological effects of electromagnetic treated water., Homeopathy., 103(3), 186-192.
  22. Kitazawa K., Ikezoe Y., Uetake H. and Hirota N. (2001)., Magnetic Field effects on water, air and powders., Physica B., 294-295, 709-714.