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Isolation and Identification of Fungi Microbiome in oral Cavity of Students in Ebonyi State University, Abakaliki, Nigeria

Author Affiliations

  • 1Department of Biological Sciences, Faculty of Science, Ebonyi State University, Abakaliki, Ebonyi, Nigeria

Int. Res. J. Biological Sci., Volume 5, Issue (7), Pages 85-87, July,10 (2016)


Microbiomes occupy different parts of the body including the oral cavity and they influence health and diseases. Studies on isolation and identification of culturable fungal microbiome prevalent in oral cavity of students in Ebonyi State University, Abakaliki was carried out in November, 2015. The aim of the study was to determine the most prevalent culturable fungi species inhabiting the oral cavity of healthy students. Swab sticks were employed for the collection of oral samples and inoculated onto potato dextrose agar (PDA) media and incubated for 72 hours at 250C, fungal species were isolated and identified. Fungi species identified were Candida, Aspergillus , Fusarium, Blastomyces and Histoplasma. The research revealed that Candida spp. and Aspergillus spp. were the most preponderant fungi organisms in oral cavity of healthy students.


  1. Oever J.O. and Netea M.G. (2014)., The bacteriome-mycobiome interaction and antifungal host defence., European Journal of Immunology, 44, 3182-3191
  2. Floyd E., Dewhirst, Tuste C., Jacques I., Anne C.R., Tanner, Wen-Han Yu, Abirami Lakshmanan and William G. Wade (2010)., The human Oral Microbiome., Journal of Bacteriology, 192(19), 5002-5017, doi:10.1128/JB. 00542-10.
  3. Dupuny A.K., David M.S., Li L., Heider T.N., Peterson J.D., Montano E.A., Dongari B.A., Diaz, P.I. and Strausbaugh L.D. (2014)., Redefining the human oral mycobiome with improved practices in amplicon-based taxonomy: discovery of malassezia as a prominent commensal., PLoS One doi: 9(3), e90899, 10.1371/journal. pone.0090899.
  4. Aas J.A., Paster B.J., Stokes L.N., Olsen I. and Dewhirst F.E. (2005)., Defining the normal Bacterial flora of the oral cavity., Journal of Clinical Microbiology, 43(11), 5721-5732
  5. Jenkinson H. and Douglas L. (2002)., Interactions between Candida species and bacteria in mixed infections., Brogden K.A. Polymicrobial diseases. Washington, DC, ASM Press.
  6. Coleman D.C., Rinaldi M.G., Haynes K.A., Rex J.A, Summerbell R.C., Anaussie E.J., Li, A. and Sullivan D.J. (1998)., Importance of Candida species other than Candida albicans as opportunistic pathogens., Med Mycol. 36(1), 156-165.
  7. Ghannoum M.A., Jurevic R.J., Mukherjee P.K., Cui F., Sikaroodi M., Naqvi A. and Gillevet P.M. (2010)., Characterization of the oral fungal microbiome (mycobiome) in healthy individuals., PLoS Pathog., 6, e1000713.
  8. Pfaller M.A. and Diekema D.J. (2010)., Epidemiology of invasive mycoses in North America., Crit Rev Microbiol 36, 1-53.
  9. Diaz P.I., Xie Z., Sobue T., Thompson A., Biyikoglu B., Ricker A., Ikonomou,L. and Dongari-Bagtzogbu A. (2014)., Synergistic interaction between Candida albicans and commensal oral streptococci in a novel in vitro mucosal model., Infect. Immun. 80, 620-632.
  10. Costa K.R., Ferreira J.C, Lavrador M.A., Baruffi M.D. and Candido R.C. (2012)., Virulence attributes and genetic variability of oral Candida albicans and Candida tropicalis isolates., Mycoses, 55, 97-105.
  11. Lalla R.V., Patton L.L. and Dongari-Bagtzoglou A. (2013)., Oral candidiasis: pathogenesis., clinical presentation, diagnosis and treatment strategies, J. Calif. Dent. Assoc., 41, 263-268.
  12. Deepa A.G., Nair B.J., Sivakumar T.T. and Joseph A.P. (2014)., Uncommon opportunistic fungal infection of oral cavity: A review., J. Oral Maxillofac Pathol, 18(2), 235-243.
  13. Cho H., Lee K.H., Colquhoun A.N. and Evans S.A. (2010)., Invasive oral Aspergillosis in a patient with acute myeloid leukemia., Aust Dent. J., 55(2), 214-218.
  14. Bathoorn E., Salazar N.E., Sepehrkhouy S., Meijer M., Cock H. and Haas P. (2013)., Involvement of the opportunistic pathogen Aspergillus tubingensis in osteomyelitis of the maxillary bone: A case report., BMC Infect Dis, 13, 59, DOI: 10.1186/1471-2334-13-59.
  15. Ge Y., Caufield P.W., Fisch G.S. and Li Y. (2010)., Streptococcus mutans and Streptococcus sanguinis colonization correlated with caries experience in children., Caries Res,. 42, 444-448.