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Bioremediation of Anthracene by Aspergillus niger and Penicillium Funiculosum

Author Affiliations

  • 1Department of Environment and Pollution, Marshes Research Centre, Thi-Qar University, Iraq

Int. Res. J. Biological Sci., Volume 5, Issue (6), Pages 1-11, June,10 (2016)

Abstract

Five filamentous fungi isolated from the upper surface of sediments of center marshes (Abu-Sabayat marshe) in four stations S1,S2,S3,S4 in AI-Nasiriya governorate by using dilution method. The results showed that Aspergillus niger and Peniclllium funiculosum were more common fungi among other fungi isolated in this study with high frequency. The appearance percent of A. niger, Peniclllium funiculosum reached to 100%, and A.versicolor appear with 75%, but the percent of A.ostianus reached to 62.5%, as well as the results obtained that A. fumigatus appear with low percent (25%). The results showed that the different sites in present study were no high effect on fungal diversity in a sediments of marshe because the similarity of environmental conditions in study sites. The results showed that A.niger, P.funicculosum were the highest resistance to anthracene. The colony diameters were calculated in 2, 5, 7 days in the 2.0 mg of anthracene. Statistical methods obtained no significant differences between fungi, but obtained significant with the time of incubation with anthracene . The dry weight of both fungi were also resistant to anthracene. Mixed to fungal was appear A.niger + P.funiculosum the highest resistance to 2.0 mg anthracene in mineral salts medium. Also the mycelial dry weight of axenic culture of of P.funiculosum was higher than control ,but the mycelia dry weight of of axenic culture of A.niger was lower than control. The statistical methods obtained non significant differences between fungi in polluted liquid medium with anthracene. The results showed that both fungi can degraded anthracene to other compounds.

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