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Insecticidal Activity of Tinospora rumphii Plant Powder Against Maize Weevil, Sitophilus zeamais

Author Affiliations

  • 1Department of Biology, College of Arts and Sciences, Cebu Normal University, Cebu City, Philippines
  • 2Department of Biology, College of Arts and Sciences, Cebu Normal University, Cebu City, Philippines

Int. Res. J. Biological Sci., Volume 5, Issue (4), Pages 37-44, April,10 (2016)

Abstract

The insecticidal activity of Tinospora rumphii stem powder was evaluated on corn weevil, Sitophilus zeamais M. under laboratory condition. Phytochemical analysis of the plant stem was conducted to determine the active toxic compounds. Three various concentrations (6%, 12% and 18%) of T. rumphii powder were exposed to corn grains containing the mature weevils within 120 hours. Results revealed that T. rumphii stem contain the following phytochemicals: flavonoids, alkaloids, steroids and tannins. Tinospora rumphii powder showed significant insecticidal activity against maize weevil as compared to the control group at 0.05 level of significance. In addition, the mortality of maize weevil treated with the plant extract is concentration-dependent effect since the percentage of weevil’s mortality increase in relation to the increase of extract concentration. Higher concentrations of the extract had stronger insecticidal effect than the lower concentrations. The corn seeds treated with the plant extract shows the least number of holes (0.25 to 0.75 out of 10 seeds) compared to the negative control (10.25 out of 10 seeds). This indicates that the plant extract has a preventive effect against weevil from damaging the corn seeds. Makabuhay extract also showed a concentration-dependent in terms of the percentage weight loss of corn grains infected with the weevil. The significant decrease of weight loss in treatment/experimental groups as compared to the negative control could be a result of higher corn weevil’s mortality. Moreover, result also reveals a significant difference, p = 0.000 on the percentage of germination among the control groups and those treated with various concentrations of the plant extract. The lowest percentage of germination was observed in the negative control (15 %) which is extremely low than those treated with the plant extract and the positive control, on the other hand, the highest concentration of the plant extract showed the highest percentage of germination (95%). Hence, T. rumphii stem powder possess insecticidal activity against maize weevil, S. zeamais.

References

  1. Asawalam E.F. (2006)., Insecticidal and repellent properties of Piper guineense seed oil extract for the control of maize weevil, Sitophilus zeamais., Electronic Journal of Entomology and Agricultural Food Chemistry, 5(3), 1389-1394.
  2. Ogunsina OO., Oladimeji MO. and Lajide L. (2011)., Insceticidal action of hexane extracts of three plants against bean weevil, Sitophilus zeamais (F.) and maize weevil, Sitophilus zeamais Motsch., J. Ecol. Nat. Environ, 3(1), 23–28.
  3. Nukenine E.N., Monglo B., Awason I., Ngamo L.S.T, Thuenguem F.F.N and Ngassoum M.B. (2002)., Armer, Journal of Biological and Biochemical Science, 12(1), 18-30.
  4. Arannilewa S.T., Ekrakene T. and Akinneye J.O. (2006)., Laboratory evaluation of four medicinal plants as protectants against the maize weevil, Sitophilus zeamais (Mots)., African Journal of Biotechnology, 5(21), 2032-2036.
  5. Storey C.L. (1987)., Effect and control of insects affecting corn quality., In S.A. Watson and P.E. Ramstad (Eds.). Corn Chemistry and Technology, 185-199. American. SPSS (2003). Statistical Package for Social Sciences, 10.0 for windows. SPSS Inc. Illinios, USA.
  6. Lajide L., Adedire C.O, Muse W.A and Agele S.O. (1998)., Insecticidal activity of powders of some Nigerian plants against the maize weevil, Sitophilus zeamais Motsch., In: Lale, N.E.S., Molta, N.B., Donli, P.O., Dike, M.C. and Aminu – Kano, M. (Eds.). Entomology in the Nigerian Economy: Research Focus in the 21st Century. Entomological Society of Nigeria (ESN), Maiduguri, Nigeria, ESN Ocassional Publication. 31, 227-235.
  7. Tadesse A. and Basedow T. (2005)., Laboratory and field studies on the effect of natural control measures against insect pests in stored maize in Ethiopia., J. Plant Dis. Prot., 112(2), 156–172.
  8. Adedire C.O. (2003)., Use of nutmeg Myristica fragans (Houtt.) powder and oil for the control of cowpea storage bruchid, Callosobruchus maculatus (Fabricius)., Journal of Plant Diseases and Protection, 109(2), 193–199.
  9. Al-Moajel N.H. (2006)., Use of Sesbania sesban (L.) Merr seed extract for the protection of wheat grain against the granary weevil, Sitophilus granarius (L.) (Coleoptera: Curculionidae)., Scientific Journal of King Faisal niversity (Basic and Applied Sciences), 7(2), 121-136.
  10. Arannilewa S.T., Ekrakene T. and Akinneye J.O. (2006)., Laboratory evaluation of four medicinal plants as protectants against the maize weevil, Sitophilus zeamais (Mots)., African Journal of Biotechnology, 5(21), 2032-2036.
  11. Huang Y., Lam S.L. and Ho S.H. (2000)., Bioactivities of essential oil from Elletaria cardamomum (L.) Maton. to Sitophilus zeamais Motschulsky and Tribolium mcastaneum (Herbst)., Journal of Stored Products Research, 36, 07–117.
  12. Koschier E.H., Sedy K.A. and Novak J. (2002)., Influence of plant volatiles on feeding damage caused by the onion thrips Thrips tabaci., Crop Protection, 21, 419–425.
  13. Asawalam E.F. and Hassanali A. (2006)., Constituents of the essential oil of Vernonia amygdalina as maize weevil protectants., Tropical and Subtropical Agroecosystems, 6, 95-1002.
  14. Salazar N.P., Sabordo N.T., Romero R.C., Montalban C.S., Acacio M.R., Tarrayo M.G., Bustos D.G. and Moragas T.R. (1987)., Tinospora rumphii Boerl., (Makabuhay) in the treatment of scabies. Phil J. Microbial Infect Dis, 16(1), 25-29.
  15. Fernandez T.J. Jr. (1996)., Panyawan (Tinospora rumphii) as dewormer for goats, Regional R and D [Research and Development] Symposia., undefined
  16. Gutierrez P. Jr., Antepuesto A., Eugenio B. and Santos M. (2014)., Larvicidal Activity of Selected Plant Extracts against the Dengue vector, Aedes aegypti Mosquito., International Research Journal of Biological Sciences. 3(4), 23-32.
  17. Harborne J. (1998)., Phytochemical methods: A Guide to Modern techniques of plant analysis., London: Kluwer Academic Publishers.
  18. Adedire C.O., Obembe O.M., Akinkurolere R.O. and Oduleye S.O. (2011)., Response Callosobruchus maculates Fabricius (Coleoptera: Chrysomelidae: Bruchinae) to extracts of cashew kernels., Journal of Plant Diseases Protection, 118(2), 75-79.
  19. Ileke K.D. and Oni M.O. (2011)., Toxicity of some plant powders to maize weevil, Sitophilus zeamais (motschulsky) [Coleopteran: Curculiondae] on stored wheat grains (Triticum aestivum)., Afr. J. Agric. Res., 6, 3043-3048.
  20. Howard A.F.B., Zhou G. and Omlin F.X. (2007)., Malaria mosquito control using edible fish in western Kenya: preliminary findings of a controlled study., BMC Public Health. 7. 199-204.
  21. Venketachalam M.R. and Jebasan A. (2001)., Repellent activity of Ferronia elephantum Corr, (Rutaceae) leaf extract against Aedes aegypti., Biores Technol. 76(3). 287-288.
  22. Rupp M., M.E. da S. Cruz, K.R.F. Schwan-Estrada, S.P. Souza Junior, J.C.T. Collella, M.J. da S. Cruz1 and A.C. Fiori-Tutida1. (2005)., Toxic effect of vegetable extracts on adults of Sitophilus zeamais Mots., 1855 (Col., Curculionidae). 9th International Working Conference on Stored Product Protection 890 PS7-31 – 6291
  23. Stangarlin J.R., Kuhn O.J. and Schwan-Estrada KRF. (2005)., Control de doencas de plantas por extratos de origem vegetal., Revis Anu Patol Plantas. 16, 265–304.
  24. Azmathullah N. Md., Asrar Sheriff M. and Sultan Mohideen A.K. (2011)., Phytochemical Screening of Calotropisprocera Flower Extracts and Their Bio-Control Potential on Culex sp. Mosquito Larvae and Pupae, International J. of Pharmaceutical and Biological Archives, 2(6), 1718-1721.
  25. Pieta P. (2000)., Flavonoids as antioxidants., Journal of Natural Products, 63, 1035-1042.
  26. Hopkins W. and Huner N. (2009)., Introduction to Plant physiology., 4th ed. John Wiley and Sons, Inc.
  27. Khanna V.G. and Kannabiran K. (2007)., Larvicidal effect of Hemidesmusindicus, Gymnema sylvestre, and Ecliptaprostrataagainst Culex qinquifaciatus mosquito larvae., African Journal of Biotechnology, 6(3), 307-311.
  28. Kogan M. (1986)., Natural chemicals in plant resistance to insects., Iowa State J. Res., 60, 501-527.
  29. Ge N. (2014)., Toxic and antifeedant activities of prenylated flavonoids isolated from Tephrosia apollinea L. against three major coleopteran pests of stored grains with reference to their structure-activity relationship., Nat Prod Res. 2014, 28(24), 2245-52.
  30. Mahmood Z.A., Ahmed S., Ahmed S.W. and Hasan M. (2012)., Insecticidal Activity of Castanospermum australe against stored Grain Pest Callosobruchus analis., IOSR Journal of Pharmacy. 2(2), 189-191.
  31. Akinbuluma M., Yeye E. and Ewete. F. (2015)., Qualitative Phytochemical screening of Acalypha fimbriata, and its methanol extract as protectant against Sitophilus zeamais (Coleoptera: Curculionidae) on stored maize., Journal of Natural Sciences Research. 5(6).
  32. Adeniyi S., Orjiekwe C., Ehiagbonare J. and Arimah B. (2010)., Phytochemical screening and insecticidal activity of leaf extracts of Bryophyllum pinnatum and Eucalptus globules against rice weevil (Sitophilus oryzae)., Int. J. Biol. Chem. Sci. 4(1), 241-246.
  33. Rejesus M.B., H.A. Maini and C.M.G arcia. (1987)., The Insecticidal Actions of Some Indigenous Plants with Special Reference to Makabuhai (Tinospora rumphii)., Trans. Nat. Acad. Sci and Tech. (Phils.), 9, 189-208.
  34. Gemechu F., Santiago D. and Sori W. (2013)., Laboratory Evaluation of Cotton (Gossypium hirsutum) and Ethiopian Mustard (Brassica cariata) Seed Oils as Grain Protectants against Maize Weevil, Sitophilus zeamais M. (Coleoptera:Curculionidae)., African Journal of Agricultural Research. 8(32), 4374-4379.