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Isolation and Identification of α- Amylase activity Inhibiting compounds from Bryophyllum Pinnatum

Author Affiliations

  • 1Department of Biotechnology, G.B. Pant Engineering College, Pauri Garhwal, Uttarakhand, India
  • 2Department of Biotechnology, G.B. Pant Engineering College, Pauri Garhwal, Uttarakhand, India
  • 3Department of Biotechnology, G.B. Pant Engineering College, Pauri Garhwal, Uttarakhand, India

Int. Res. J. Biological Sci., Volume 5, Issue (10), Pages 21-27, October,10 (2016)

Abstract

Bryophyllum pinnatum (B. Pinnatum) is an herb growing widely and utilized in folkloric medication in tropical Africa, tropical America, India, China, and Australia. Aqueous extract of B. pinnatum leaf was tested for alpha amylase inhibition properties which showed significant inhibitory activity against alpha amylase. B. pinnatum found to have maximum alpha amylase inhibitory activity at a concentration of 100µg/ml. A compound Digitoxin showed maximum binding energy with alpha amylase comparative to drug Metformin, Metformin at concentration 100 µg/ml showed 67.34±0.002% inhibitory effects on the alpha-amylase activity with an IC50 value 3.25µg/ml. The alpha-amylase inhibitory activity of 79.41±0.002% with an IC50 value 2.54µg/ml was shown by aqueous leaf extract of B. pinnatum at a concentration 100 µg/ml. The plant extract showed potential alpha-amylase inhibitory activity in a dose dependent manner.

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