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Seed storage Protein profiling of Pea (pisum sativum l.) Genotypes using SDS-PAGE

Author Affiliations

  • 1Dept. of Vegetable Science, G.B. Pant University of Agriculture and Technology, Pantnagar-263145, Uttarakhand, INDIA

Int. Res. J. Biological Sci., Volume 5, Issue (1), Pages 37-41, January,10 (2016)

Abstract

Ten pea genotypes of different origin from were investigated for genetic divergence based on seed protein profile using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) in G.B. Pant University of Agriculture and Technology, Pantnagar during 2012-13. Based on seed proteins, 13 subunits were observed and among these 7 were polymorphic. The un-weighted paired group method with arithmetic averages (UPGMA) exhibited 2 clusters which were further grouped into distinct subgroups. Further, bifurcation at different levels leads to the different similarity index groups at the 90 per cent. In broader spectrum, the genotypes from various sources differed in grouping and it was difficult to establish relationship between origin and cluster pattern. The protein banding data were investigated in relation to agronomic traits that indicated influence of polymorphic bands on quantitative traits. Particular clusters were better for specific traits that are suggested to utilize in crop improvement program.

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