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Phenolic composition, Antioxidant activity and FT-IR Spectroscopic Analysis of Halophyte Sesuvium portulacastrum L. Extract

Author Affiliations

  • 1Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai, 608 502, Tamil Nadu, INDIA

Int. Res. J. Biological Sci., Volume 5, Issue (1), Pages 1-13, January,10 (2016)

Abstract

Sesuvium portulacastrum L. commonly known as ‘sea pursulane’ of family Aizaceae is a good source of food and conventional medicine. The aim of the present work was to analyse the total phenolic compounds (TPC) and to observe its antioxidant capacity, in four different extract using hexane, dichloromethane (DCM), ethyl acetate and methanol solvents respectively. Results presented that extraction with Hexane (0.6± 0.03 gm) and DCM (0.5 ± 0.02 gm) showed the highest yield. The methanol extract revealed highest amount (40.75 mg/g dry wt. Gallic acid equivalent) of TPC. Extract in DCM showed maximum reducing power (0.350±0.009)s and minimum (0.239±0.009) in hexane extracts was observed. Also, DCM showed the highest antioxidant activity of 112.95 ± 0.087 mg/g dry wt. Ascorbic acid equivalent, followed by ethyl acetate (83.56 ± 0.25 mg/g dry wt. Ascorbic acid equivalent). Among the four extracts as mentioned above, in DPPH (2, 2-diphenyl-1-picryl hydrazyl) assay methanol extract revealed maximum efficacy with respect of scavenging ability (75.1%). Here DCM fraction was found powerful inhibitor (84.76%) in comparison to hydroxyl radical at 1000μg/mL concentration. The maximum hydrogen peroxide radical scavenging activity (83%) was showed by DCM fraction. The FT-IR spectrum confirmed its functional group, responsible for antioxidant potential of the halophyte S. portulacastrum. The findings of the present research work helped us to identify the proper solvent for extracting phenolic compounds from the halophyte which may provide a rich source of natural antioxidants in food industry.

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