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A new Bacterial strain Streptomyces indiaensis (LMG 19961) and its larvicidal and Histopathological effect against Anopheles stephensi: A Malaria Mosquito

Author Affiliations

  • 1Department of Zoology, University College of Science, Mohan Lal Sukhadia, University, Udaipur, Rajasthan, INDIA

Int. Res. J. Biological Sci., Volume 4, Issue (4), Pages 43-48, April,10 (2015)

Abstract

The present study was carried out to establish larvicidal activity (LC50 determination) and mode of action of a soil bacteria Streptomyces indiaensis strain (LMG 19961)by taking midgut as a target tissue against third instar larvae of Anopheles stephensi (L.), the vector of malaria. LC50 of Streptomyces indiaensis was calculated by biotoxicity test. The LC50 (1.645 cfu/ml) treated third instar larvae showed dose dependent effect on mortality. The histoarchitecture of midgut revealed weak and disoriented epithelial cells with larger intercellular spaces and disruption of junctional complexes. The lysed gastric caecal cells were also noticed and reduced lumen further revealed the severity of bacterial strain against mosquito larvae. The peritrophic membrane was dislodged from the place. Since this strain of Streptomyces indiaensis was reported first time in our laboratory (NCBI Accession number KJ170314) the probability of ecosafe vector control agents from soil bacteria is quite encouraging.

References

  1. WHO report., WHO Globla Malaria Programme, (2012)
  2. WHO report., Current states of Malaria, (2011)
  3. Christophers S.R., Aedes aegypti (L) The yellow fever mosquito: Its life history, bionomics and structure. Cambridge University Press, Cambridge, (1960)
  4. Knight K.L. and Stone A.A., catalog of the mosquitoes of the World. Geo. W. King Co, Baltimore, MD (1977)
  5. Tabachnick W.J., Evolutionary genetics and the yellow fever mosquito, Am. Entomol., 37, 14–24 (1991)
  6. Mattingl Y.P.F. and Knight K.L., The mosquito of Arabia, I. Bull. Br. Mus. (Nat. Hist.)., 91-141 (1956)
  7. Büttiker W., Observations on urban mosquitoes in Saudi Arabia. Fauna of Saudi Arabia., 3, 472-479 (1981)
  8. Abdoon A.M. and Ibrahim A.A., Mosquito breeding habitats in Tihama lowlands of Asir region, Kingdom of Saudi Arabia. Proceedings of the 3rd Conference of Applied Entomology, 1-18 (2005)
  9. ALkhreji M.A., Survey and distribution of mosquito species (Diptera: Culicidae) and description of its habitat in Riyadh district, Kingdom of Saudi Arabia. M.Sc. thesis, King Saud University, Kingdom of Saudi Arabia, (2005)
  10. Ahmed A.M., Shaalan E.A., Aboul-soud M.A.M., Tripet F. and AL-khedhairy A.A., Mosquito vectors survey in Al-Ahsaa district, eastern region, Kingdom of Saudi Arabia. J. Insect Sci., 11, 1-11 (2011)
  11. Piyusha G., Shelar S., Reddy VKS., Shelar GS. and Vidhyasagar Reddy, Medicinal value of Mangroves and its antimicrobial properties - A review. Continental J of Fisheries and Aquatic science, 6(1), 26-37 (2012)
  12. WHO, Insecticide resistance in arthropods (eds. A.W.A. Brown and R. Pal). A monograph series, 38 (2nd edition) (1971)
  13. Hecker H., Structure and function of midgut epithelial cells in culicidae mosquitoes (Insecta,Diptera), Cell and tissue research, 184, 3, 321-341 (1977)
  14. World Health Organization, Global Strategic Framework for Integrated Vector Management, Geneva, Available: http://whqlibdoc.who.int/hq/2004/ WHOCDSCPEPVC 10, (2006)
  15. World Health Organization, World Malaria Report, ISBN9789241564533, (2012)
  16. Finney D.J., Probit Analysis. Cambridge Univ. Press, Cambridge, 333, (1971)
  17. Zieler H., Garon C.F., Fischer E.R. and Shahabuddin M.A., tubular network associated with the brush-border surface of the Aedes aegypti midgut: Implications for pathogen transmission by mosquitoes. The Journal of Experimental Biology, 203, 1599-1611 (2000)
  18. Volkman A. and Peters W, Investigations on the midgut caeca of mosquito larvae, I-Fine structure, Tissue Cell, 21: 243-251. (1989a)
  19. Zhuang Z., Paul J., Linser and William R. Harvey, Antibody to H+ V-atpase subunit E colocalizes with portasomes in alkaline larval midgut of a freshwater mosquito (Aedes aegypti (L), The Journal of Experimental Biology, 202, 2449–2460 (1999)
  20. Fillinger U., Knols B.G.J. and Becker N., Efficacy and efficiency of new Bacillus thuringiensis var. israelensis and Bacillussphaericus formulations against Afrotropical anophelines in western Kenya, Trop Med Int Health, (8), 37–47 (2003)
  21. Becker N. and Margalit J., Use of Bacillus thuringiensis israelensis against mosquitoes and black flies. In: Bacillus thuringiensis, An environmental biopesticide: Theory and practice (eds. P.F. Entwhistle, J.S. Cory, M.J. Bailey and S. Higgs), Wiley, New York, 255–266 (1993)
  22. Clark T.M., Koch A. and Moffett D.F., The anterior and posterior ‘Stomach’regions of larval Aedes aegypti midgut: regional specialization of ion transport and stimulation by 5- hydroxytryptamine, J. Exp. Biol202, 247-252 (1999)
  23. Rey D., Pautou M.P. and Meyran J.C., Histopathological effects of tannic acid on the midgut epithelium of some aquatic dipteral larvae, Journal of Invertebrate Pathology, 73, 173-181 (1999)
  24. Gill S.S., Cowles E.A. and Pietrantonio P.V., The mode of action of Bacillus thuringiensis endotoxins, Annu. Rev. Ent., 37, 615-636 (1992)
  25. Allured V.S., Collier R.J., Carroll S.F. and Mckay D.B., Structure of exotoxin A of Pseudomonous aeruginosa at 3.0-ngstrom resolution, Proc. natl. Acad. Sci. USA, 83, 1320-1324 (1986)
  26. Bravo A., Gill S.S. and Soberón M., Mode of action of Bacillus thuringiensis Cry and Cyt toxins and their potential for insect control, Toxicon, 49, 423–435 (2007)
  27. AL-mehmadi R.M. and AL-khalaf A.A., Larvicidal and histological effects of Melia azadarach extract on Culex quinquefasciatus Say larvae (Diptera: Culicidae), J. King Saud Univ. (Sci.), 22, 77–85 (2010)
  28. Ndione R.D., Faye O., Ndiaye M., Dieye A. and Afoutou J.M., Toxic effects of neem products Azadirachta indica A. Juss on Aedesaegypti Linnaeus 1762 larvae, African Journal of Biotechnology, 6, 24, 2846-2854 (2007)
  29. Ferreira C., Ribeiro A.F. and Terra W.R, Fine structure of the larval midgut of the fly Rhynchosciara and its physiological implications, J. Insect Physiol, 27, 559-570 (1981)
  30. Lehane M.J., Peritrophic matrix structure and function, Annu. rev. Entomol, 42, 525-50 (1997)