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Methi Lutein prevents Oxidative Stress induced DNA Damage and Cytotoxicity

Author Affiliations

  • 1Adichunchanagiri Bitotechnology and Cancer Research Institute, B.G.Nagara-571448, Mandya, Karnataka, INDIA

Int. Res. J. Biological Sci., Volume 4, Issue (2), Pages 54-61, February,10 (2015)

Abstract

The detrimental effect of oxidative stress has been known since a long, which may cause the deleterious effect on DNA and finally leads to death. The present investigation revealed the DNA protectant effect of Lutein anti-oxidant purified from fresh Methi (Trigonellafoenumgraecum) leaves. Ferrous sulphate and ascorbic acid (10:100µM) induced the lipid peroxidation and fragmentation in DNA in human lymphocytes model systems. Methi lutein inhibits the formation of lipid peroxidation and DNA fragmentation in fresh human lymphocytes to the magnitude of 80% and 76% respectively. On treatment of Cow dung smoke condensate with human lymphocytes could lead to the adduct formation in human DNA which was controlled by the Methi Lutein (20M) effectively. U.V radiation induced DNA double strand break and cross linking was strongly controlled by the Methi lutein in Calf thymus DNA when compared to the standard antioxidants BHA and -tocopherol. Methi lutein might also avoid the cytotoxicity induced by the H2 and Fe:AA in fresh lymphocytes by maintaining the 80% viability even after 2 hours of treatment.

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