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Electrophoretic Analysis in two groups of enzymes of Musca domestica L. (Diptera: Muscidae)

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Author Affiliations

  • 1Department of Zoology, Iswar Saran Degree College, University of Allahabad, Allahabad-211004, INDIA
  • 2Department of Zoology, CMP Degree College, University of Allahabad, Allahabad-211002, INDIA

Int. Res. J. Biological Sci., Volume 4, Issue (10), Pages 20-23, October,10 (2015)

Abstract

Indian population of Musca domestica was examined by PAGE for allozyme variation. One to five enzyme loci in the glucose metabolizing system (Group I) and six to fourteen enzyme loci in the non-glucose metabolizing system (group II) were assayed. The parameter estimated were the allele frequencies, proportion of polymorphic loci and average heterozygosity for Group I and Group II loci. The present finding is that the genetic variability measured by allozyme variation is much higher for group II than for group I enzymes in M.domestica population. The loci coding for the hydrolytic and other nonspecific enzymes are much more variable than the loci coding for the enzymes of the glycolytic pathway, Kreb’s cycle and other specific enzymes.

References

  1. Gillespie J.H. and Kojima K., The degree of polymorphism in enzymes involved in energy production compared to that in nonspecific enzymes in two Drosophila ananassae populations, Proc. Nat. Acad. Sci. U.S.A.61, 582-585 (1968)
  2. Kojima K., Gillespie J. and Tobari Y.N., A profile of Drosophila species enzymes assayed by electrophoresis, I. Number of Alleles, heterozygosities, and linkage disequilibrium in glucose-metabolizing systems and some other enzymes, Biochem. Genet., 627-637 (1970)
  3. Gillespie J.H. and Langley C.H., A general model to account for enzyme variation in natural populations, Genetics,76, 837-848 (1974)
  4. Band H.T., A survey of isozyme polymorphism in a Drosophila melanogaster natural population, Genetics, 80, 761-771 (1975)
  5. Prakash S, Patterns of gene variation in central and marginal populations of Drosophila robusta, Genetics, 75, 347- 369 (1973)
  6. Singh K. and Thakur S., A survey of polymorphism in laboratory population of S. dux (Diptera , Sarcophagidae), 3(2), 638 -644 (2012)
  7. Allendorf F.W., Luikart G. and Aitken S.N., Genetic variation in natural populations: chromosomes and proteins. In: Conservation and the Genetics of Populations, Allendorf F. W., Luikart G. and Aitken S. N. (eds) Blackwell Publishing Ltd, 35–53 (2013)
  8. Stanger J., Preliminary observations on genetic variation in three colonies of Musca domestica (Diptera: Muscidae) isolated from central Alberta, Quaest. Entomol., 20, 51-59 (1984)
  9. Black IV W.C. and Krafsur E.S., Electrophoretic analysis of genetic variability in the house fly (Musca domestica L.),Biochem. Genet, 23(3-4), 193-203 (1985)
  10. Krafsur E.S., Helm J.M. and Black IV W.C., Genetic diversity at electrophoretic loci in the house fly, Musca domestica L. Biochem. Genet.,30, 317-328 (1992)
  11. Krafsur E.S., Bryant N.L., Marquez J.G. and Griffith N.T., Genetic distances among North American, British and West African house fly populations Musca domesticaL. Biochem, Genet., 38, 275-284 (2000)
  12. Taskin B.G., Taskin V. and Kucukakyuz K., Electrophoretic analysis of genetic diversity in natural house fly (Musca domestica L.) populations from the Western and Southern coasts of Turkey, Tubitak ,Turk. J. Biol., 35, 337-346 (2011a)
  13. Taskin B.G., Taskin V., Kucukakyuz K. and Kence M., Determination of esterase enzyme polymorphism in house fly (Musca domestica.) populations in Turkey, Tubitak, Turk. J. Zool., 35(6), 869-877 (2011 b)
  14. Tripathi M., Tewari R.R. and Agrawal U.R., Genetic variations in house fly, Musca domesticaL (Diptera :Mucsidae) from Allahabad India, Proc. Nat. Acad. Sciences, India (Biological Sciences), 80(1), 24-29 (2010)
  15. Tripathi M., Agrawal U.R. and Tewari R.R., Seasonal genetic variation in house fly populations, Musca domestica, (Diptera :Mucsidae), Cell. Mol. Biol., 57, 129-134 (2011)
  16. Tripathi M., Agrawal U.R., Tripathi J and Tewari R.R., Spatial genetic variation in house fly populations, Musca domestica (Diptera :Mucsidae). ). Int. J. Pharm. Bio. Sci., 3(4), 927 – 934 (2012)
  17. Tripathi M., Agrawal U.R., and Tripathi J., Allozyme variation in house fly populations, Musca domestica from Allahabad, India, I. Res. J. Biological Sci., 2(11), 37–40 (2013)
  18. Ayala F.J., Powell J.R., Tracey M.L., Mourao C.A. and Perez-Salas S., Enzyme variability in the Drosophila willistoni group IV, Genic variation in natural populations of Drosophila willistoni, Genetics., 70, 113-139 (1972)
  19. Tsukamoto M., Enhancement of Staining intensity of mosquito larva zymograms after electrophoresis. J. UOEH., 11, 461-479 (1989)
  20. Tsukamoto M. and Horio M., Electrophoretic comparison of the lactate dehydrogenase banding pattern among Japanese mosquito larvae (Diptera: Culicidae), J. Med. Entomol., 22, 491-498 (1985)
  21. Berlocher S.H., An Electrophoretic key for distinguishing species of the genus Rhagoletis (Diptera : Tephritidae) as larvae, pupae, or adults, Ann. Entomol. Soc. Am., 73, 131-137 (1980)
  22. Tripathi M., Genetic variation in house fly, Musca domestica (Muscidae: Diptera), D. Phil. dissertation, University of Allahabad, (2007)
  23. Matsuoka N and Nakamura Y., Enzyme variation within the population of the sea urchin, Glyptocidaris crenularis, from Japanese water, Comp. Biochem. Physiol.B., 96(2), 335-338 (1990)
  24. Matsuoka N. and Hatanaka T., Molecular evidence for the existence of four sibling species within the sea urchin, Echinometra mathaei in Japanese waters and their evolutionary relationships. Zool. Sci., 121-133, (1991)
  25. Asanuma T. and Matsuoka N., Phylogenetic relationships among seven fish species of the order Clupeiformes inferred from allozyme variation, Bull. Fac. Agric. and Life Sci., Hirosaki Univ. 4, 1-15, (2002)
  26. Matsuoka N, Molecular Phylogeny and Allozyme Variation of the Five Common Fish Species of the Suborder Percoidei. Bull. Fac. Agric. and Life Sci., Hirosaki Univ., 5, 17-22 (2003)
  27. Yamazaki T., Enzyme polymorphism and functional difference: mean, variance and distribution of heterozygosity. In: Molecular Evolution and Polymorphism, Mishima: National Institute of Genetics, Mishima, M. Kimura (eds), 127–147 (1977)
  28. Gojobari T., Means and variances of heterozygosity and protein function. In: Molecular Evolution, Protein Polymorphism and the Neutral Theory, M. Kimura (eds), Japan Scientific Societies Press, Springer-Verlag, Berlin, 137–148 (1982)
  29. Frydenberg O. and Simonsen V., Genetics of Zoarces populations, V. Amount of protein polymorphism and degree of genic heterozygosity, Hereditas, 75, 221-232 (1973)
  30. Kimura M., The Neutral Theory of Molecular Evolution, Cambridge University Press, Cambridge, (1983)