6th International Virtual Congress (IVC-2019) And Workshop.  International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Genetic Relationship between the Muscids Using RAPD-PCR as marker

Author Affiliations

  • 1Cytogenetics Laboratory, Department of Zoology, University of Allahabad, UP, INDIA
  • 2Department of Zoology, C.M.P. Degree College, Allahabad, UP, INDIA

Int. Res. J. Biological Sci., Volume 4, Issue (1), Pages 66-70, January,10 (2015)

Abstract

Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) markers have become the most common yardsticks to study genetic relatedness and to resolve intra and inter-specific relationship in many insect groups. In the present study, RAPD-PCR has been used to infer genetic relationship between the two muscid species i.e., housefly Musca domestica (L.) and pepper fruit fly Atherigona orientalis. Genomic DNA from the two species were extracted and PCR amplified using twenty arbitrary decamer primers which produced 127 reproducible and scorable bands ranging from 121bp to 2568bp. Genetic identity between the two species was calculated using Tools for population genetic analysis(TFPGA) software. High values of Genetic identity (0.680) display close relationship between the two species.

References

  1. Ferreira M.J.M. and Lacerda P.V., Muscóides sinantrópicos associados ao lixo urbano em Goiânia, GO. Rev. Bras. Zool., 10, 185-195 (1993)
  2. Scott J.G., Liu N., Kristensen M. and Clark A.G., A case for sequencing of the genome of Musca domestica (Diptera: Muscidae)., J. Med. Entomol., 46(2), 175-182 (2009)
  3. Greenberg B., Flies and disease. Ecology, Classification and biotic associations New Jersey: Univ. Press Princeton., 1 (1970)
  4. Greenberg B., Flies and disease, Biology, disease transmission New Jersey : Univ. Press Princeton, 2, (1973)
  5. Welsh J. and McClelland M., Fingerprinting genomes using PCR with arbitrary primers, Nucleic Acids Res., 18(24), 7213-7218 (1990)
  6. Infante-Malachias M.E., Yotoko K.S.C. and Azeredo-Espin A.M.L., Random amplified polymorphic DNA of screwworm fly populations (Diptera: Calliphoridae) from Southeastern Brazil and Northern Argentina, Genome, 40, 772-779 (1999)
  7. Brito L.G., Regitano L.C.A., Huacca M.E.F., Carrilho E., Paes M.J. and Borja G.E., Genotype characterization of the Haematobia irritans (Diptera : Muscidae) from Brazil, Dominican Republic and Colombia based on randomly amplified polymorphic DNA (RAPD) analysis, Revista Brasileira de Parasitologia Veterinaria, 17(4), 179-184 (2008)
  8. Skoda S.R, Figarola J.L., Pornkulwat S. and Foster J.E., Inter- and intra-specific identification of the screwworm, Cochliomyia hominivorax, using random amplified polymorphic DNA-polymerase chain reaction, J. Insect Sci., 13(76), 1-15 (2013)
  9. Vasconcelos S.M., Campos A.P.S. Franco M.M. andGoulart Filho L.R., Selecao de primers RAPD para characterizacao de divergencia genetica intrapopulational em Haematobia irritans. Arquivos Instituto Biologico., 71, 1-749 (2004)
  10. Castiglioni L. and Bicudo H.E.M.C. Molecular characterization and relatedness of Haematobia irritans (hornfly) populations, by RAPD-PCR. Genetica, 124, 11-21(2005)
  11. Bajpai N. and Tewari R.R. Genetic characterization of sarcophagid flies by Random Amplified Polymorphic DNA- Polymerase Chain Reaction (RAPD-PCR), Natl. Acad. Sci. Lett., 33, 3-4 (2010)
  12. Malviya S., Bajpai N. and Tewari R.R. Genetic Relatedness among three populations of housefly, Musca domestica L. using RAPD - PCR marker, Int. J. Pharm. BioSci.,2(4), 198-204 (2011)
  13. Malviya S., Bajpai N. and Tewari R.R. RAPD-PCR Based genetic relationship of muscid flies (Diptera: Muscidae), Int. J. Pharma and BioSci., 3(3), 1018-1024 (2012)
  14. Xu M., Sun S., Wang R. and Li S. Genetic variation and phylogenetic relationships among six populations of corn borers in China, Biochem.Genetics., 36, 289-297 (1998)
  15. Sambrook J., Fritsch E.F. and Maniatis T. Molecular cloning. A laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, p. 6.3-6.34. Cold Spring Harbor Laboratory Press, New York (1989)
  16. Miller M.P. Tools for population genetic analysis (TFPGA) (http://bioweb.usu.edu/mpmbiol). (1997)
  17. Nei M. Genetic distance between populations. Am. Nat., 106, 283-292 (1972)
  18. Chandra G., Saxena A. and Barat A. Genetic diversity of two riverine populations of Eutropiichthys vacha (Hamilton, 1822) using RAPD markers and implications for its Conservation, J. Cell Mol. Biol.,8(2), 77-85 (2010)
  19. Santos J.M.M., Fraga E.C., Maia J.F. and Tadei W.P. Genetic Diversity in Dengue Mosquito, Aedes aegypti (Diptera: Culicidae) from Amazon Region : Comparative Analysis with Isozymes and RAPD Loci, Open Trop. Med. J.,11-20 (2011)
  20. Williams J.G., Kubelik A.R., Livak K.J., Rafalski J.A. and Tingey S.V. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers, Nucleic Acids Res., 18(22), 6531-6535 (1990)
  21. Williams K.P. and Bartel D.P., PCR product with strands of unequal length, Nucleic Acids Res., 23(20), 4220-4221 (1995)
  22. Hadrys H., Balick M. and Schierwater B. Applications of random amplified polymorphic DNA (RAPD) in molecular ecology, Mol. Ecol.,, 55-63 (1992)
  23. Krafsur E.S., Helm J.M. and Black IV, W.C. Genetic diversity at electrophoretic loci in the house fly, Musca domestica L. Biochem. Genet., 30, 317-328 (1992)
  24. Szalanski A.L., Taylor D.B. and Peterson II, R.D. Population genetics and gene variation of stable fly populations (Diptera: Muscidae) in Nebraska, J. Med. Entomol., 33, 413-420 (1996)
  25. McDonald P.T., Hilburn L.R. and King S.E., Genetic similarities among natural populations of the horn fly (Diptera: Muscidae), Ann. Entomol. Soc. Am., 80, 288-292 (1987)
  26. Li W.H. and Graur D., Fundamentals of Molecular Evolution, Sunderland, Massachusetts, Sinauer Associates (1991)
  27. Santos J.M.M., Rodriguez G.A.D., Maia J.F. and Tadei W.P., Variability and genetic differentiation among Anopheles(Ano) intermedius Chagas, 1908 and Anopheles(Ano) mattogrossensis Lutz and Neiva, 1911 (Diptera: Culicidae ) from the Brazilian Amazon, Mem. Inst.Oswaldo. Cruz.,100, 631-637 (2005)
  28. Sharma A.K., Mendki M.J., Tikar S.N., Chandel K., Sukumaran D., Parashar B.D., Vijay Veer, Agarwal O.P. and Shri Prakash, Genetic variability in the geographical populations of Culex quinquifasciatus Say (Diptera: Culicidae) from India based on random amplified polymorphic DNA analysis, Acta tropica., 112, 71-76 (2009)
  29. Haymer D.S., Mei H. and McInnis D.O., Genetic marker analysis of spatial and temporal relationships among existing populations and new infestations of the Mediterranean fruit fly (Ceratitis capitata), Heredity79, 302-309 (1997)
  30. Shinonaga S. and Tewari R.R., Record of the muscid flies collected in India, SriLanka and Bangladesh (Diptera, Muscidae), Jap. J. Syst. Entomol., 14(2), 205-251 (2008)