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Protective Effect of Curcumin on Diethanolamine-Induced Toxic Effects on Human Spermatozoa: An in Vitro Study

Author Affiliations

  • 1Department of Zoology, University School of Sciences, Gujarat University, Ahmedabad-380009, INDIA

Int. Res. J. Biological Sci., Volume 3, Issue (7), Pages 34-39, July,10 (2014)


Curcumin is a yellow pigment from Curcuma longa which has desirable preventive or putative therapeutic properties. In this study protective effect of curcumin on diethanolamine-induced toxicity on human spermatozoa in in vitro condition was investigated. For this study samples were collected from normal healthy donors. After liquefaction, samples were used for preparation of sperm suspension to evaluate sperm motility, sperm viability and sperm morphology. Statistical analysis was performed using analysis of variance (ANOVA) followed by Tukey’s test and the level of significance was accepted with p0.05. When sperm suspension was treated with diethanolamine (300 µg/ml) it caused significant decrease in sperm motility and sperm viability as compared to control. Treatment also caused significantly increased different kinds of sperm morphological abnormalities as compared to control. Addition of different concentrations (10-40 µg/ml) of curcumin to sperm suspension along with diethanolamine caused significant increase in sperm motility and sperm viability as compared to treated which was time-dependent as well as concentration-dependent. As compared to treated, concentration-dependent decrease in various kinds of morphological abnormalities were also observed. This findings clearly indicate that curcumin ameliorates diethanolamine-induced spermatotoxic effect on human spermatozoa.


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