International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Isolation and Characterization of Keratinolytic Bacteria from Poultry farm soils

    ,

Author Affiliations

  • 1Department of Microbiology, K.R.P. Kanya Mahavidyalaya, Islampur, Dist- Sangli Maharashtra, INDIA
  • 2Shri. Jagdishprasad Jhabarmal Tibrewala University, Zhunjhunu, Rajasthan, INDA

Int. Res. J. Biological Sci., Volume 3, Issue (7), Pages 29-33, July,10 (2014)

Abstract

Keratinolytic microorganisms have a great importance in poultry waste degradation and its bioconversion to compost or animal feed. The present study aimed at selection of keratin degrading bacteria from poultry soil. The poultry farm soil samples were added in basal medium with feathers as a source of carbon and nitrogen. The enriched samples were streaked on nutrient agar and hichromebacillus agar for isolation of keratinolytic bacteria.15 isolates were obtained. All the isolates were screened for their keratinolytic potential on milk agar. Out of fifteen isolates five were keratinolytic.Five isolates were further studied for feather degradationin shake flasks.Of the five, four showed complete degradation of feather barbules within 120 h and one isolate showed degradation within 72 h. The isolate was identified morphologically and by 16 s rRNA sequencing as Breuvendimonosterrae. It produced maximum keratinase at 37 C temperature and at 130 rpm speed within 72 h. Breuvendimonosterrae showed 76U/ml enzyme activity.The bacterium can be used for biotechnological purpose.

References

  1. Yammura S., Y. Morita, Q. Hasan, K. Yokoyama and E. Tamiya, Keratin degradation a Cooperative action of two enzymes from Stenotrophomonossp. Biochem, Biophys. Res. Commun,294, 1138-1143 (2002)
  2. Onifade A.A., N.A. Al-Sane, A.A. Al-Mussallan and S. Alzarban, Potentials for biotechnological applications of keratin degrading microorganisms and their enzymesforNutritional improvements of feathers and other keratins as livestock feed resources, Bioresource. Technol,66, 1-11 (1998)
  3. Wang J.J. and Shih J.C.H., Fermentation production of keratinase from Bacillus licheniformis PED-1 and Bacillus subtilis FDB-29, J.Ind. Microbiol Biotechnol,22, 608-619 (1999)
  4. Takami, H.H. Satoshi, R. Aono and K. Horikoshi, Degradation of human hair by a thermostable alkaline Protease from alkalophilic Bacillus sp, no.AH-101, Biosci.Biotechnol.Biochem,56, 1667-1669 (1992)
  5. Ession J.P., A.A. Umoh, E.J. Akpan, S.J. Eduok and A. Umoiyoho, Keratinolytic Proteinase activity and thermo tolerance of dermatophytes associated with Alopecia in Uyo, Nigeria, Acta Microbiological Et Immunologica Hungarica, 56, 69 (2009)
  6. Kaul S. and Sumbali G.G., Keratinolysis by Poultry farm Soil Fungi, Mycopathologia 139, 137-140 (1557)
  7. Cai C., B. Lou and X. Zheng, Keratinase Production and Keratin degradation by Mutant Strain of Bacillus subtilis, J. Zhejiang Univ.ScI.B., 60-67 (2008)
  8. P. Jeevana Lakshmi, Ch. M. Kumari Chitturi and V.V. Lakshmi, Efficient degradation of feather by keratinase producing Bacillus sp. International journal of Microbiology, Article ID 608321, (2013)
  9. Sreenivasa Nayaka and Vidyasagar G.M., Purification and characterization of Keratinase from Native feather degrading Streptomyces albus, International journal of Development Research, 3(8), 034-039 (2013)
  10. Sarita Agrahari and Neeraj Wadhwa, Degradation of chicken feather, a poultry waste Product by Keratinolytic Bacteria Isolated from Dumping Site at Ghazipur Poultry processing plant, International Journal of Poultry science, 9(5), 482-489 (2010)
  11. Lowry O.H., Rosebrough N.J., Farr A.L. and Randall R.J., Protein measurement with the Folin phenol reagent, J. Biochem, 193(1), 265-75 (1951)
  12. K. Salvam and Vishnupriya B., Biochemical and Molecular characterization of Microbial Keratinase, International journal of Pharmacutical and Biological Archives, 3(2),267-275 (2012)
  13. Friedrich J., Gradisar H., Mandian D. and Chaumont J.P., Screening Fungi for Synthesis of Keratinolytic Enzymes, Letter in Applied Microbiology, 127-130 (1999)
  14. Vigneshwaran C., Shanmugam S. and Sathish Kumar T.,Screening and Characterization of Keratinase from Bacillus licheniformis isolated from namakkal Poultry farm, 2(4), 89-96 (2010)
  15. Bockle B.B. Galunski and R. Muller, Characterization of a Keratinolytic serine Protease from Streptomyces Pactum dsm 40530, Appl.Environ Microbiol,61, 3505-3710 (1995)
  16. Lee.C.G., Ferket.P.R. and Shih.C.H. Improvement of feather degradation by Bacterial Keratinase AS A Feed Additive, fasebJ,59, 1312 (1991)
  17. Riffel A.A. Brandelli, P. Heeb and F.Lucas, Characterization of new Keratinolytic bacterium that degrades native feather keratin, Arch, Microbial, 179, 258-265 (2003)
  18. Jeong J.H., Park K.H., Oh D.J., Hwang D.Y., Kim H.S., Lee C.Y., Son H.J., Keratinolytic enzyme-mediated biodegrdation of recalcitrant feather by a newly isolated Xanthomonos sp.P5. Polym. degrad.Stab.,95, 1969-1977 (2010b)
  19. Minghai Han, Wei Luo, Qiuy Gu and Xiaobin Yu. Isolation and characterization of Keratinolytic Proteaswe from a feather degrading bacterium Pseudomonos aeruginosa C 11, African Journal of Microbiology Research, 6(9), 2211-2221, (2012)
  20. Revathi K, Shaifali Singh, Mohd Azzem Khan and Suneetha V., A potential strain of keratinolytic Bacteria VIT RSAS2 from Katpadi and its Pharmacological Benefits, International Journal of Pharmacutical sciences Review and Research, 20(2),14, 89-92 (2013)