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Isolation and Characterization of Keratinolytic Bacteria from Poultry farm soils

Author Affiliations

  • 1Department of Microbiology, K.R.P. Kanya Mahavidyalaya, Islampur, Dist- Sangli Maharashtra, INDIA
  • 2Shri. Jagdishprasad Jhabarmal Tibrewala University, Zhunjhunu, Rajasthan, INDA

Int. Res. J. Biological Sci., Volume 3, Issue (7), Pages 29-33, July,10 (2014)

Abstract

Keratinolytic microorganisms have a great importance in poultry waste degradation and its bioconversion to compost or animal feed. The present study aimed at selection of keratin degrading bacteria from poultry soil. The poultry farm soil samples were added in basal medium with feathers as a source of carbon and nitrogen. The enriched samples were streaked on nutrient agar and hichromebacillus agar for isolation of keratinolytic bacteria.15 isolates were obtained. All the isolates were screened for their keratinolytic potential on milk agar. Out of fifteen isolates five were keratinolytic.Five isolates were further studied for feather degradationin shake flasks.Of the five, four showed complete degradation of feather barbules within 120 h and one isolate showed degradation within 72 h. The isolate was identified morphologically and by 16 s rRNA sequencing as Breuvendimonosterrae. It produced maximum keratinase at 37 C temperature and at 130 rpm speed within 72 h. Breuvendimonosterrae showed 76U/ml enzyme activity.The bacterium can be used for biotechnological purpose.

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