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Genetic Analysis of Y-chromosomal STRs in Khandayat Population of Odisha, INDIA

Author Affiliations

  • 1Amity Institute of Forensic Sciences (AIFS), Amity University, Noida, U.P., INDIA
  • 2Shriram Institute for Industrial Research, New Delhi, INDIA

Int. Res. J. Biological Sci., Volume 3, Issue (7), Pages 26-28, July,10 (2014)

Abstract

This study was conducted to analyze haplotype diversity Khandayat Community of Odisha, INDIA using Y-chromosome specific short tandem repeat (Y-STR) loci. We collected blood samples from 106 unrelated healthy men of Khandayat Community and isolation of DNA was carried out by standard phenol-chloroform extraction method. Genomic DNA samples were amplified by multiplex-PCR using AmpFl STR Yfiler PCR Amplification Kit for 12 Y-STR loci followed by genotyping. A total of 97 haplotypes were identified among 106 individuals, out of which 93 were unique. Allelic frequency and gene diversity (GD) were calculated for each Y-STR locus. The resulting haplotype diversity (HD) was found to be 0.9798. Discrimination capacity (DC) was observed to be 0.9135. Haplotypes of Khandayat population of Odisha were compared with that of other Indian populations using AMOVA (Analysis of molecular variance) tool in order to measure the genetic relatedness between different populations.

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