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A comparative study on Glutathione S-transferase activities of the Needles of Two Pine species under Drought and Cold stresses

Author Affiliations

  • 1Department of Biology, Faculty of Science, Yuzuncu Yil University (YYU), Van-TURKEY

Int. Res. J. Biological Sci., Volume 3, Issue (7), Pages 65-70, July,10 (2014)


Total cytosolic Glutathione S-transferase (GST, EC activities and the changes in the GSH pools of the needle samples from cold-resistant Pinus sylvestris and relatively drought resistant Pinus brutia species were screened for three seasons. Samples of spring season were accepted as reference for other two groups of sampling done in summer and winter; and, daily total precipitation, air humidity, minimum and maximum temperature values and freeze incidents were recorded. Results revealed the possible correlation between the dimensions of GSH pools and total GST activities of needle homogenates at cold and drought stresses. Those two species showed different behaviours for the same climatic conditions.


  1. Gezer A., The Sylviculture of Pinus brutia in Turkey, Technical Report for CIHEAM, Paris, (1986)
  2. Labra M., Grassi F., Sgorbati S. and Ferrari C., Distribution of genetic variability in southern populations of Scots pine (Pinus sylvestris L.) from the Alps to the Apennines, Flora, 201(6), 468-476 (2006)
  3. Sutinena M.L., Repob T., Sutinenc S., Lasarovd H., Alvilad L. and Pakkanen T.T., Physiological changes in Pinus sylvestris needles during early spring under sub-arctic conditions, Forest. Ecol. Manag., 135(1-3), 217-228 (2000)
  4. Marrs K.A., The functions and regulation of Glutathione S-transferases in plants, Annu. Rev. Plant Physiol. Plant Mol. Biol., 47, 127-158 (1996)
  5. Seppanen M., Cardi T., Hyökki B.M. and Pehu E., Characterization and expression of cold-induced glutathione S-transferase in freezing tolerant Solanum commersonii, sensitive S. tuberosum and their interspecific somatic hybrids, Plant Sci., 153(2), 125-133 (2000)
  6. Warade W.N., Levels of Glutathione S-Transferasein Different Larval Tissues of Papilio Demoleus, Res. J. Recent. Sci., 1(ISC-2011) , 313-316 (2012)
  7. Bose P. and Bathri R., Glutathione S-Transferase gene polymorphisms (GSTT1, GSTM1, GSTP1) as increased risk factors for asthma and COPD among Isocyanate exposed population of Bhopal, India, Res. J. Recent. Sci., 1(ISC-2011), 219-223 (2012)
  8. Yadav S.K., Cold stress tolerance mechanisms in plants, A review, Agron. Sustain. Dev., 30(3), 515-527 (2010)
  9. Galant A., Preuss M.L., Cameron J.C. and Joseph M.J., Plant glutathione biosynthesis: diversity in biochemical regulation and reaction products, Front. Plant Sci., 45 (2011)
  10. Schröder P. and Berkau C., Characterization of cytosolic glutathione S-transferase in spruce needles, Botanica Acta, 106(4), 301-306 (1993)
  11. Lowry O., Rosebrough N., Farr A. and Randall R., Protein measurement with the Folin phenol reagent, J. Biol. Chem., 193(1), 265-275 (1951)
  12. Grill D., Pfeifhofer H. and Esterbauer H., Further investigations on the thiol content of Norway spruce needles, Phyton (Austria), 27(2), 311-317 (1987)
  13. Pukacka S. and Pukacki P.M., Seasonal changes in antioxidant level of Scots pine (Pinus sylvestris L.) needles exposed to industrial pollution, I. Ascorbate and thiol content, Acta Physiol. Plant, 22(4), 451-456 (2000)
  14. Sedlak J. and Lindsay R., Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent, Anal. Biochem., 25(1), 192-205 (1968)
  15. Habig W., Pabst M., Jakoby W., Glutathione S-transferases. The first enzymatic step in mercapturic acid formation, J. Biol. Chem., 249(22), 7130-7139 (1974)
  16. Noctor G., Mhamdi A., Chaouch S., Han Y., Neukermans J. and Garcia B.M., Glutathione in plants: an integrated overview, Plant Cell Environ., 35(2), 454-484 (2012)
  17. Kawamura Y. and Uemura M., Mass spectrometric approach for identifying putative plasma membrane proteins of Arabidopsis leaves associated with cold acclimation, Plant J., 36, 141-154 (2003)
  18. Kosmala A., Bocian A., Rapacz M., Jurczyk B. and Zwierzykowski Z., Identification of leaf proteins differentially accumulated during cold acclimation between Festuca pratensis plants with distinct levels of frost tolerance, J. Exp. Bot., 60, 3595-3609 (2009)
  19. Manoj K. and Padhy P.K., Oxidative Stress and Heavy Metals: An Appraisal with Reference to Environmental Biology, Int. Res. J. Biological Sci.,2(10), 91-101 (2013)
  20. Davis D.G. and Swanson H.R., Activity of stress-related enzymes in the perennial weed leafy spurge (Euphorbia esula L.), Environ. Exp. Bot., 46, 95-108 (2001)
  21. Hausladen A. and Alscher R.G., Cold-Hardiness-Specific Glutathione Reductase lsozymes in Red Spruce-Thermal Dependence of Kinetic Parameters and Possible Regulatory Mechanisms, Plant Physiol., 105, 215-223 (1994)
  22. Rigling, A., Brühlhart, H., Bräker, O. U., Forater, T., Schweingruber, F. H., Effects of irrigation on diameter growth and vertical resin duct production in Pinus sylvestris L. on dry sites in the central Alps, Switzerland, Forest Ecol. Manag.,175 (1-3), 285-296,(2003)
  23. Gruber A., Pirkebner D., Florian C. and Oberhuber W., No evidence for depletion of carbohydrate pools in Scots pine Pinus sylvestris L.) under drought stress, Plant Biol., 14 (1), 142-148 (2012)
  24. Bigler C., Bräker O., Bugmann H., Dobbertin M., Rigling A., Drought as an inciting mortality factor in Scots pine stands of the Valais, Switzerland, Ecosystems, 330-343 (2006)