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A new improved Stability-Indicating RP-HPLC method for Determination of Diosmin and hesperidin in combination

Author Affiliations

  • 1Department of Pharmaceutics, College of Pharmacy, Salman Bin Abdulaziz University, Al-kharj, Kingdom of Saudi Arabia
  • 2Department of Pharmacogonosy, College of Pharmacy, Salman Bin Abdulaziz University, Al-kharj, Kingdom of Saudi Arabia
  • 3Department of Pharmacogonosy, College of Pharmacy, Alexandria University, Alexandria, EGYPT

Int. Res. J. Biological Sci., Volume 3, Issue (6), Pages 41-46, June,10 (2014)


A rapid, simple, precise and cost effective stability-indicating RP-HPLC method has been developed and validated for determination of diosmin and hesperidin in combination. The mobile phase consisted of mixture of methanol: water (45:55 v/v). UV detection was performed at 346 nm. The method was linear over the concentration ranges 4.8-7.2 g mL-1, and 20-40 g mL-1 with correlation coefficient 0.999 both for diosmin and hesperidin. The developed method was validated as per ICH guidelines. The recovery of diosmin and hesperidin were in the range of 100.3 to 102.2 %. The limit of detection and limit of quantitation were 0.0102 and 0.0311g mL-1for diosmin and 0.0558 and 0.1693 g mL-1for hesperidin respectively. The proposed method is reproducible, accurate, robust and suitable for the simultaneous quantitative analysis of the studied drugs in bulk and dosage formulation.


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