9th International Science Congress (ISC-2019).  International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Antioxidant Potential of Tinospora cordifolia Climber from Eucalyptus hybrid, Ficus religiosa and Roystonea regia

Author Affiliations

  • 1Department of Biotechnology & Biosciences, Lovely Professional University, Ludhiana-Jhalandhar G.T. Road, Phagwara, Punjab-144402, INDIA

Int. Res. J. Biological Sci., Volume 3, Issue (5), Pages 5-9, May,10 (2014)


Stems of Tinospora cordifolia were collected from different hosts namely, Eucalyptus hybrid, Ficus religiosa and Roystonea regia. To evaluate antioxidant potential, various parameters such as total reducing power, DPPH radical scavenging activity, lipid peroxidation inhibitory activity and superoxide radical scavenging assay were performed. Stems collected from the host Ficus religiosa showed highest phenolic content 5.25 mg/gm and hence higher reducing ability. Highest antioxidant activity of 41.73 µM Fe (II)/g and EC50 value for DPPH radical scavenging activity was also observed in stems collected from Ficus religiosa (0.6 mg/ml).


  1. Maffei M., Dietary supplements of plant origin-Nutrition and health approach. Taylor and Francis e-library 18 (2003)
  2. Mathew S. and Kuttan G., Antioxidant activity of Tinospora cordifolia and its usefulness in the amelioration of cyclophosphamide-induced toxicity, J Exp Clin Cancer Res,16, 407- 411 (1997)
  3. Sohni Y.R., Kaimol P., Bhatt R.M., The antiamoebic effect of a crude drug formulation of herbal extract against anti amoebahistolytica In vitro and In vivo.J Ethnopharm45, 43 (1995)
  4. Prince P.S. and Menon V.P., Antioxidant activity of Tinospora cordifolia roots in experimental diabete, J Ethnopharma, 65, 277-281 (1999)
  5. Bandyopadhyay U., Das D. and Banerjee R.K., Reactive oxygen species: oxidative damage and pathogenesis, Curr Sci, 77, 658-65 (1999)
  6. Cheung L.M., Cheung P.C.K. and Ooi V.E.C., Antioxidant activity and total phenolic of edible mushroom extracts, Food Chem, 81, 249-255 (2003)
  7. Higenaga M.K., Hagen T.M., Oxidants, antioxidants and the degenerative diseases of aging, Proc of the Nat. Acad. of Sci. of the Unt. States. of Am., 90, 7915-7922 (1993)
  8. Baruchel S. and Wainberg M.A., The role of oxidative stress in disease progression in individuals injected by human deficiency virus, J Leukocyte Bio, 52, 111-114 (1992)
  9. Elliot R.H. and Strunin L., Hepatotoxicity of volatile anaesthetics, Br J Anaesthesia, 70, 339-349 (1993)
  10. Halliwell B., Free radicals and anti-oxidants: A Personal View, Nut Review, 52, 253-265 (1994)
  11. Fry D.E., Multiple system organ failure. (Ed) Mosby Year Book St Louis Missouri, 3-14 (1992)
  12. Parke D.V., The pathogenesis of inflammatory disease: surgical shock and multiple system organ failure, Inflammo Pharma,149-168 (1995)
  13. Salvimini D., Wang Z.Q., Bovedon D.M., Ster M.K., Currie M.C., Manniy P.T., Evidence of peroxinitrite involvement in the carragenan induced rat paw oedema, Eur J Pharmacol303, 217 (1996)
  14. Kaur C., Kapoor H.C., Antioxidants in fruits and vegetables – the millennium’s health, Int J Food Sci Technol,36,703-725 (2001)
  15. Ivanova D., Gerova D., Chervenkov T., Yankova T., Polyphenols and antioxidant capacity of Bulgarian medicinal plants, J Ethnopharmacol, 97, 145-150 (2005)
  16. Edge R., Mc Greevy D.J., Truscott T.G., The carotenoids as antioxidants: A review, J Photochem Photobiol B Biol, 189-200 (1997)
  17. Jha A., Kaur P., Khan, M.A., Siddiqui S., Sharma N.R., Viola species and Tinospora cordifolia as Herbal Antibiotics, Journal of Pharmacy Research, 5, 4, 2286-2289 (2012)
  18. Sharma N.R., Bala J., Singh A., Kaur P., Antibacterial Potential of Achyranthus aspera Linn Procured from Himachal Pradesh, Punjab and Haryana, India, Research Journal of Chemical Sciences, 1(8), 80-82, Nov. (2011)
  19. Benzie I.F.F., Strain J.J., The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power: The FRAP assay, Ana Biochem, 239, 70-76 (1996)
  20. Premanath R., Lakshmidevi N., Studies on Anti-oxidant activity of Tinospora cordifolia (Miers.) leaves using in vitro models, J Am Sci, 10 (2010)
  21. Malick C.P., Singh M.B., In: Plant Enzymology and Histo-Enzymology, Kalyani Publishers New Delhi, 286 (1980)
  22. Yen G.C., Duh P.D., Antioxidative properties of methanolic extracts from peanut hulls, J Am Oil Chemists Soc,70, 383-386 (1993)
  23. Moon J.H., Terao J., Antioxidant activity of caffeic acid and dihydrocaffeic acid in lard and human low density protein, J Agr Food Chem,46, 5062-5065 (1998)
  24. Fontana M., Mosca L., Rosei M.A., Interaction of enkephalines with oxyradicals, Biochem Pharmacol, 61, 1253-1257 (2001)
  25. Kroon P., Williamson G., Polyphenols: Dietary components with established benefits to health, J Sci Food Agri,85, 1239–1240 (2005)
  26. Kessler M., Ubeaud G., Jung L., Anti- and pro-oxidant activity of rutin and quercetin derivatives, J Pharm Pharmacol55, 131-142 (2003)
  27. Wilhelm R., Klaus K. and Juergen S., Method for increasing the content of flavonoids and phenolic substances in plants, BASF AG, Patent AO1N37/42 (2000)
  28. Kontogiorgis A.C., Pontiki A.E., Hadjipavlou-Litina D A., Review on quantitative structure–activity relationships (QSAR) of natural and synthetic antioxidant compounds, Mini-Reviews Med Chem, 563–574 (2005)
  29. Nenadis N., Zhang, Tsimidou H.Y.M.Z., Structure antioxidant activity relationships of ferulic acid derivatives: Effect of carbon side chain characteristic groups, J Ag and Food Chem, 57, 874–1879 (2003)
  30. Demitray S., Pintado M.E., Castro P.M.L., Evaluation of phenolic profiles and antioxidant activities of Turkish medicinal plants: Tiliaargentea, Crataegi folium leaves and Polygo numbistorta roots, World Acad Sci Engg Technol, 54, 312-317 (2009)
  31. Sirisha N., Sreenivasulu M., Sangeeta K.C., Madhusudhana C., Antioxidant Properties of Ficus Species - A Review, Int J Pharm Tech Res, 2174-2182 (2010)
  32. Luo Q,, Cai Y., Sun M., Corke H., Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer, Life Sci, 74, 2157-2184 (2004)
  33. Pitchaon M., Suttajit M., Pongsawatmani R., Assessment of phenolic content and free radical scavenging capacity of some Thai indigenous plants, Food Chem,,1409-1418 (2007)
  34. Pokorny J., Yanishlieva N., Gordan M., Antioxidants in food: Practical applications, Woodhead Publishing Ltd Cambridge, 3 (2001)
  35. Bhawya D. and Anilkumar K.R., In Vitro Antioxidant potency of Tinospora cordifolia (gulancha) in sequential extracts, Int J pharm Biol Arch, 448-456 (2010)