6th International Virtual Congress (IVC-2019) And Workshop.  International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

A strain of Chryseobacterium sp. isolated from necrotic leaf tissue of chayote (Sechium edule Jacq)

Author Affiliations

  • 1Biotechnology and AppliedEcologyInstitute (INBIOTECA), Veracruzana University, Culturas Veracruzanas No 101, Col. Emiliano Zapata CP91090, Xalapa, Veracruz, MÉXICO
  • 2 High TechnologyLaboratory of Xalapa, Veracruzana University, Médicos No. 5, Col. Unidad del Bosque, CP 91010, Xalapa, Veracruz,MÉXICO
  • 3 Faculty of Biology, Zone Xalapa, VeracruzanaUniversity, UniversityZone, Xalapa, CP 91000, Veracruz, MÉXICO

Int. Res. J. Biological Sci., Volume 3, Issue (4), Pages 52-60, April,10 (2014)

Abstract

There are few reports of bacterial diseases in chayote because these are usually attributed to other pathogens, mainly fungi. However, from two Sechium edule Jaqc Sw plants showing necrosis of the leaves, the presumptive pathogen was isolated from symptomatic leaves on yeast-dextrose-calcium carbonate agar yielding yellow-orange color colonies that developed after 36 hours. This study was proposed to identify the microorganism associated with this pathology and to evaluate its aptitude to induce necrotic damage in four varieties in S. edule. The bacterium identification was carried out by examining the characteristics of the colonies and cells, the biochemical characteristics and a sequence of the gene fragment coding 16S rRNA. It was found that leaf necrosis of chayote may be caused by Chryseobacterium sp. Similarly, a phylogenetic analysis was performed with 26 species of this genus together with the strain under study, finding more similarity to C. indologenes and C. gleum. Significant differences were found in the expression of the symptoms of chlorosis and necrosis amongst the varieties of chayote constantly appearing throughout our study. Chryseobacterium was described in 2010 for the first time as a pathogen associated with Soft Rot of the Calla Lily in Poland. This genus has been found in different environments, and therefore it is considered appropriate to investigate its ecological role or any other association it may have with plants.

References

  1. Vandamme P., Bernarder J.F., Segers P., Kersters K., Holmes B., New Perspectives in the Classification of the Flavobacteria: Description of Chryseobacterium gen. nov., Bergeyella gen. nov., and Empedobacter nom. Rev, Int J Syst Bacteriol, 44(4), 827–831 (1994)
  2. Kirby J.T., Sader H.S., Walsh T.R., Jones R.N., Antimicrobial susceptibility and epidemiology of a worldwide collection of Chryseobacterium spp: Report from the SENTRY Antimicrobial Surveillance Program (1997-2001), J Clin Microbiol,42(1), 445-448 (2004)
  3. Refik M., Aktas E., Yasemin E., Aysegul C., Durmaz R., Postoperative Chryseobacterium indologenes bloodstream infection caused by contamination of distillate water, Infect Control Hosp Epidemiol,28 (3), 9-368 (2007)
  4. 594.Zhou Y., Dong J., Wang X., Huang X., Zhang K-Y., Zhang Y-Q., Guo Yu-Feng, Lai R., Wen-Jun L., Chryseobacterium flavum sp. nov., isolated from polluted soil, Int J Syst Evol Microbiol,57, 1765–1769 (2007)
  5. Herzog P., Winkler I., Wolking D., Kämpfer P., Lipski A., Chryseobacterium ureilyticum sp. nov, Chryseobacterium gambrini sp. nov., Chryseobacterium pallidum sp. nov. and Chryseobacterium molle sp. nov., isolated from beer-bottling plants, Int J Syst Evol Microbiol,58, 26–33 (2008)
  6. Kämpfer P., Dreyer U., Neef A., Dott W., Busse H.J., Chryseobacterium defluvii sp. nov., isolated from wastewater, Int J Syst Evol Microbiol,53, 93–97 (2003)
  7. Hugo C.J., Segers P., Hoste B., Vancanneyt M., Kersters K., Chryseobacterium joostei sp. nov., isolated from the dairy environment, Int J Syst Evol Microbiol,53, 771–777 (2003)
  8. de Beer H., Hugo C.J., Jooste P.J., Willems A., Vancanneyt M., Coenye T., Vandamme P., Chryseobacterium vrystaatense sp. nov., isolated from raw chicken in a chicken-processing plant, Int J Syst Evol Microbiol, 55(5), 2149–2153 (2005)
  9. de Beer H., Hugo C.J., Jooste P.J., Vancanneyt M., Coenye T., Vandamme P., Chryseobacterium piscium sp. nov., isolated from fish of the South Atlantic Ocean off South Africa, Int J Syst Evol Microbiol,56(6), 1317–322 (2006)
  10. Park M.S., Jung S.R., Lee M.S., Kim K.O., Do J.O., Lee K.H., Kim S.B., Bae K.S., Isolation and characterization of bacteria associated with two sand dune plant species, Calystegia soldanella and Elymus mollis,J Microbiol,43, 219–227 (2005)
  11. Shen F.T., Kämpfer P., Young C.C., Lai1 Wei-An, Arun A.B., Chryseobacterium taichungense sp. nov., isolated from contaminated soil, Int J Syst Evol Microbiol, 55, 1301–1304 (2005)
  12. Tai C.J., Kuo H.P., Lee F.L., Chen H-K., Yokota A., Lo C-C., Chryseobacterium taiwanense sp. nov., isolated from soil in Taiwan, Int J Syst Evol Microbiol,56, 1771–1776 (2006)
  13. Young C.C., Kampfer P., Shen F.T., Lai W.A., Arun A.B., Chryseobacterium formosense sp. nov., isolated from the rhizosphere of Lactuca sativa L. (garden lettuce), Int J Syst Evol Microbiol, 55(1), 423–426 (2005)
  14. Hantsis-Zacharov E., Halpern M., Chryseobacterium haifense sp. nov., a psychrotolerant bacterium isolated from raw milk, Int J Syst Evol Microbiol, 57(10), 2344-2348 (2007)
  15. Quan Z.X., Kim K.K., Kim M.K., Long J., Sung-Taik L., Chryseobacterium caeni sp. nov., isolated from bioreactor sludge, Int J Syst Evol Microbiol, 57(1),141-145 (2007)
  16. Vaneechoutte M., Kämpfer P., De Baere T., Avesani V., Janssens M., Wauters G., Chryseobacterium hominis sp. nov., to accommodate clinical isolates biochemically similar to CDC groups II-h and II-c, Int J Syst Evol Microbiol, 57, 2623–2628 (2007)
  17. Yoon J.H., Kang S.J., Oh T.K., Chryseobacterium daeguense sp. nov., isolated from wastewater of a textile dye worksInt, Int J Syst Evol Microbiol, 57(6), 1355–1359 (2007)
  18. Goddard-Juárez, M.G., Hernández-Gama, R.; Rojas-Avelizapa, N.G., Identificación Molecular de Microorganismos Capaces de Remover Bifenilo Provenientes de Suelos Contaminados, Centro de Investigación en Ciencia Aplicada y Tecnología Avanzada del IPN, 3, 37-51 (2010)
  19. Gallego V., Garcia M.T., Ventosa A., Chryseobacterium hispanicum sp. nov., isolated from the drinking water distribution system of Sevilla, Spain, Int J Syst Evol Microbiol, 56, 1589-1592 (2006)
  20. Park M.S., Jung S.R., Lee K.H., Lee M.S., Do JO, Kim S.B., Bae K.S., Chryseobacterium soldanellicola sp. nov. and Chryseobacterium taeanense sp. nov., isolated from roots of sand-dune plants, Int J Syst Evol Microbiol,56(2),433–438 (2006)
  21. Weon H.Y., Kim B.Y., Yoo S.H., Kwon S.W., Cho Y.H., Go S.J., Stackebrandt E., Chryseobacterium wanjuense sp. nov., isolated from greenhouse soil in Korea, Int J Syst Evol Microbiol,56 (7), 1501-1504 (2006)
  22. Ilardi P., Fernández J., Avendaño-Herrera R., Chryseobacterium piscicola sp. nov., isolated from diseased salmonid fish, Int J Syst Evol Microbiol,59, 3001-3005 (2009)
  23. Tuon F.F., Campos L., Duboc de Almeida G., Gryschek R.C., Chryseobacterium meningosepticum as a cause of cellulitis and sepsis in an immunocompetent patient, J Med Microbiol, 56, 1116–1117 (2007)
  24. Bernardet J.F., Hugo C., Bruun B., The genus Chryseobacterium. In The Prokaryotes: an Evolving Electronic Resource for the Microbiological Community. (eds) M. Dworkin, Springer New York, USA, http://link.springer-ny.com/link/ service/books (2001)
  25. McSpadden-Gardener B.B., Weller D.M., Changes in Populations of Rhizosphere Bacteria Associated with Take-All Disease of Wheat, Appl Environ Microbiol, 67 (10), 4414–4425 (2001)
  26. Mikicinski A., Sobiczewski P., Sulikowska M., Pu awska J., Treder J., Pectolytic Bacteria Associated with Soft Rot of Calla Lily (Zantedeschia spp.) Tubers, J Phytopath, 158, 201–209 (2010)
  27. Gamboa W., Producción agroecológica una opción para el desarrollo del cultivo del Chayote (Sechium edule Jac Sw), Universidad de Costa Rica, San José Costa Rica, 219 (2005)
  28. 60Las variedades del chayote mexicano, recurso ancestral con potencial de comercialización México, Grupo Interdisciplinario de Investigación en Sechium edule en México, A.C., 88 (2010)
  29. Zar J.H., Bioestatistical analysis. Prentice Hall, New Jersey, U.S.A, 842 (1999)
  30. Fautz E., Reichenbach H., A simple test for flexirubin-type pigments, Microbiol Lett, , 87–91 (1980)
  31. Holt J.G., Krieg N.R., Sneath P.H.A., Staley J.T., Williams S.T., Bergey’s Manual of Determinative Bacteriology. Lippincott Williams Wilkins, Maryland, USA, 2816 (2000)
  32. Schaad N.W., Jones J.B., Chun W., Laboratory guide for identification of plant pathogenic bacteria, The American Phytopathological Society Press, Minnesota USA, 373 (2001)
  33. Madigan M.T., Martinko J.M., Brock biology of microorganisms, Prentice Hall, New Jersey, USA, 842 (2006)
  34. Flamm R.K., Hinrichs D.J., Thomashow M.F., Introduction of pAM31 into Listeria monocytogenes by Conjugation and Homology Between Native L. monocytogenes Plasmids, Ifect Immun,44(1), 157-161(1984)
  35. Felske A., Rheims H., Wolterink A., Stackebrandt E., Akkermans A.D.L., Ribosome analysis reveals prominent activity of an uncultured member of the class Actinobacteria in grassland soils, Microbiology,143, 2983-2989 (1997)
  36. Luna M., García S., García O., Trigos Á., Serratin a new metabolite obtained from Serratia marcescens, a bacterium isolated from the microflora associated with banana plantations, Nat Prod Lett,27(1), 49-53 (2013)
  37. Nei M., Kumar S., Molecular Evolution and Phylogenetics, Oxford University Press, New York, USA, 300 (2000)
  38. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S., MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Pasrimony Methods, Mol Biol Evol, 28, 2731-2739 (2011)
  39. Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., Higgins D.G., The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools, Nucleic Acids Res,25, 4876–4882 (1997)
  40. Mauel M.J., Miller D.L., Frazier K.S., Hinnes I.I.E., Bacterial pathogens isolated from cultured bullfrogs (Rana castesbeiana), J Vet Diagn Invest,14, 431-433 (2002)
  41. Bernardet J.F., Vancanneyt M., Matte-Tailliez O., Grisez L., Tailliez P., Bizet C., Nowakowski M., Kerouault B., and Swings J., Polyphasic study of Chryseobacterium strains isolated from diseased aquatic animals, Syst Appl Microbiol, 28, 640–660 (2005)
  42. Madriz, K.O., Mecanismos de defensa en las interacciones planta-patógeno, Manejo Integrado de Plagas63, 22–32 (2002)
  43. Cadena Iñiguez J., Hernández M.S., Arévalo Galarza Ma. de L., Avendaño-Arrazate C.H., Aguirre-Medina J. F., Ruiz-Posadas L. del Mar., Caracterización bioquímica de variedades domesticadas de Chayote Sechium edule (Jacq.) Sw. comparadas con parientes silvestres, Revista Chapingo Serie Horticultura, 2, 45-55 (2011)
  44. Bramley P., The regulation and genetic manipulation of carotenoid biosynthesis in tomato fruit, Pure Appl Chem, 69, 2159–2162 (1997)
  45. Cadena-Iñiguez J., Avendaño-Arrazate C.H., Soto-Hernández M., Ruiz-Posadas L. M., Aguirre-Medina J. F, Arévalo-Galarza L., Infraspecific variation of Sechium edule (Jacq.) Sw.in the state of Veracruz, Mexico, Genet Resour Crop Evol, 55, 835–847 (2008)
  46. Cadena-Iñiguez J., Ruiz-Posadas M.L., Aguirre-Medina J.F., Sánchez-García P., Estudio de los síntomas asociados a la pérdida de color del Chayote, Revista Chapingo Serie Horticultura, 11 (2), 309-316 (2005)
  47. Robinson, R., El retorno de la resistencia. Fitomejoramiento de los cultivos para reducirconsecuencias y estrategias de respuesta a la dependencia de plaguicidas, Editorial Grupo Chorlaví, Futura SA México, http://www.GrupoChorlavi.org/cafe http://www.sharebooks.ca/eBooks/Resistencia, 281 (1996)
  48. Altieri M.A., The ecological role of biodiversity in agroecosystems, Agricult Ecosys Environ, 74, 19–31, (1999)
  49. Dardanelli M.S., Manyani H., González-Barroso S., Rodríguez-Carvajal M.A., Gil-Serrano A.M., Espun M.R., López-Baena, F.J., Bellogín R.A., Megías M., Ollero F.J., Effect of the presence of the plant growth promoting rhizobacterium (PGPR) Chryseobacterium balustinumAur9 and salt stress in the pattern of flavonoids exuded by soybean roots, Plant Soil, 328, 483–493 (2010)
  50. Domenech J., Reddy M.S., Kloepper J.W., Ramos B., Gutiérrez-Mañero J., Combined application of the biological product LS213 with Bacillus, Pseudomonas or Chryseobacterium for growth promotion and biological control of soil-borne diseases in pepper and tomato, BioControl, 51, 245–258 (2006)