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Prevalence of Mercury-Resistant and Antibiotic-Resistant Bacteria found in Dental Amalgam

Author Affiliations

  • 1Department of Biological Sciences, College of Science and Mathematics, MSU-Iligan Institute of Technology, Iligan City, PHILIPPINES

Int. Res. J. Biological Sci., Volume 3, Issue (4), Pages 1-4, April,10 (2014)

Abstract

Mercury and antibiotic resistance has long been a subject of interest in microbiology that a vast of literature consisting of studies that looked at its genetics and molecular mechanisms. The aim of this study was to isolate and identify Mercury-resistant and antibiotic resistant bacteria and determine the Hg-resistant isolates were also antibiotic resistant. Twenty four bacterial isolates (54% Gram-negative and 46% Gram-positive) from saliva samples with amalgam fillings were screened for Mercury and resistance by cultivation in an HgCl-containing medium. Surviving organisms were identified using the conventional method of identification and susceptibility to antibiotics was determined by Kirby-Bauer disc diffusion. All of the twenty four isolates were able to grow in Mercury-containing medium and were considered as Hg-resistant bacteria. The rate of susceptibility of the bacterial isolates against the antibacterial disk showed high mean percentage in each antibiotic disk, showing that there is a growing trend of susceptibility to antibiotic which might result to antibiotic resistance. The result of the study showed that Mercury-resistant and antibiotic-resistant bacteria can be isolated from the oral saliva samples of the amalgam filled individual. This is a matter of interest for science and medicine since more and more bacterial species acquire genes that confer them resistance and presents new challenges for treating the associated conditions it cause and in eliminating the bacteria themselves.

References

  1. Koral S.M., The Scientific Case Against Amalgam, International Academy of Oral Medicine and Toxicology,(2002)
  2. Green Facts Organization, Tooth filling materials: Dental Amalgams and Alternative Materials, Scenihr/Scher Source Documents(2008)
  3. Mutter J., Is Dental Amalgam Safe for Humans? The opinion of the scientific committee of the European Commission, Journal of Occupational Medicine and Toxicology,, 2 (2011)
  4. Havarinasab S. and Hultman P., Organic mercury compounds and autoimmunity, Autoimmunity Rev., 4, 270-5 (2005)
  5. Scenihr, The safety of dental amalgam and alternative dental restoration materials for patients and users: Preliminary Report. Scientific Committee on Emerging and Newly Identified Health Risks Plenary, Available on http://ec.europa.eu/health/ph_risk/risk_en.htm (2007)
  6. Liebert C.A., Wireman J., Smith T. and Summers A.O., The Impact of mercury released from dental “silver” fillings on antibiotic resistances in the primate oral and intestinal bacterial flora, Met. Ions Biol Syst., 34, 441-460 (1997)
  7. Lorscheider F.L., Vimy M.U., Summers A.O. and Zwiers H., The dental amalgam mercury controversy-inorganic mercury and the CNS; genetic linkage of mercury and antibiotic resistances in intestinal bacteria, Toxicology,97, 12-22 (1995)
  8. Summers A.O., Wireman J., Vimy M.U., Lorscheider F.L., Marshall B. and Levy S.B., Mercury released from dental “silver” fillings provokes an increase in mercury-and antibiotic-resistant bacteria in oral and intestinal floras of primates, Antimicrob Agents Chemother., 37, 825-834 (1993)
  9. Davis U., Roberts A.P., Ready D., Richards H, Wilson, M, Mullany P. Linkage of a novel mercury resistance operon with streptomycin resistance on a conjugative plasmid Enterococcus faedium,Plasmid,54, 26-38 (2005)
  10. Skumik D., Ruimy R., Ready D., Ruppe E., Bemede-Bauduin C., Djossou F., Guillemot D., Pier G.B., Andremont A. Is exposure to mercury driving force for the carriage of antibiotic resistance genes? Journal Med Microbiol., 59, 804-807 (2010)
  11. Radha K., Mahima R., Ramanathan G., and Thangapandian V. Survey on Drug Resistant Pattern of Clinical Isolates and Effect of Plant Extract on the Drug Resistant Pattern, ISCA Journal of Biological Sciences, 1(3), 14-19 (2012)
  12. Nakade D.B., Antibiotic sensitivity of common Bacterial Pathogens against selected Quinolones, ISCA Journal of Biological Sciences,1(1), 77-79 (2012)
  13. Sotero-Martins A., de Jesus M.S., Lacerda M, Moreira J.C., Filguerias A.L.L., and Barrocas P.R.G. A conservative region of the Mercuric Reductase Gene (MERA) as a Molecular Marker of Bacterial Mercury Resistance, Brazilian Journal of Microbiology,39, 307-310 (2008)
  14. Bass L., Liebert C.A., Lee M.D., Summers A.O., White D.G., Thayer S.G., and Maurer J.J. Incidence and Characterization of Integrons, Genetic Elements, Mediating Multiple-Drug Resistance, in Avian Escherichia coli,Antimicrobial Agents and Chemotherapy,43, 2925-2929 (1999)
  15. Essa A.M.M., Julian D.J., Kidd S.P., Brown N.L., and Hobman J.L. Mercury Resistance Determinants Relatedd to Tn21, Tn1696, and Tn5053 in Enterobacteria from the Preantibiotic Era, Antimicrobial Agents Chemother,47, 1115-1119 (2003)