5th International Young Scientist Congress (IYSC-2019).  International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Evaluation of Microbial Quality of Street Vended Vegetable and Fruit Juices

Author Affiliations

  • 1 School of Biotechnology, Vignan University, Vadlamudi, Guntur Dt., AP, INDIA

Int. Res. J. Biological Sci., Volume 3, Issue (3), Pages 60-64, March,10 (2014)


Millions of people throughout the world consume fresh juices sold by street vendors as they are rich source of nutrients such as vitamins and minerals. They cause a great health problem in unhygienic conditions. There are reports of food borne illness associated with the consumption of street vended juices throughout the world and at several places in India. The present study was undertaken to evaluate the quality of juices sold by street vendors in Guntur, A.P., India. Two types of vegetable juices namely beetroot and carrot; three types of fruit juices namely mousambi, grapes and pineapple were selected based on the consumers demand. The juices are collected from five different localities viz., Brundavan Gardens, Sankar vilas, RTC Bus terminus, Kothapet and Nazz center. TVC (Total Viable Count), coliforms and yeast counts were analysed using standard methods like serial dilution and plate count. The results showed that TVC was highest in carrot juice followed by beetroot, pineapple, grape and mousambi juices respectively. TVC count ranged from 87-250 (x 10 CFU/ml) in beetroot juice; 80-271 in carrot juice; 15-150 in mousambi juice; 19-184 in grape juice and 25-217 in pineapple juice. The coliform count ranged from 8-66 (x 105 CFU/ml) in beetroot juice; 1-54 in carrot juice; 0-73 in mousambi juice; 1-10 in grape juice and 1-63 in pineapple juice respectively. However, the coliform count was highest in mousambi juice followed by beetroot, pineapple, carrot and grape juices. The yeast count ranged from 11-106 (x 10 CFU/ml) in beetroot juice; 5-41 in carrot juice; 1-53 in mousambi juice, 3-20 in grape juice and 7-126 in pineapple juice respectively. The yeast count was highest in pineapple juice followed by beetroot, mousambi, carrot, and grape juice respectively. It is concluded that imparting training to the street vendors and creating awareness regarding good manufacturing practices (GMP) and good hygienic practices (GHP), implementation of standard hygienic practices and regular monitoring of the quality of fruit juices must be introduced to avoid frequent pathogen outbreaks.


  1. Bagde N.I. and Tumane P.M., Studies on microbial flora of fruit juices and cold drinks, Asiatic J. Biotechnology Resources, (4), 454-460 (2011)
  2. Sunday P. Ukwo , Nyaudoh U. Ndaeyo. and Etido J. Udoh ., Microbiological quality and safety evaluation of fresh juices and edible ice sold in Uyo Metropolis, South-South, Nigeria, Internet J. Food Safety, 13,374-378 (2011)
  3. Al-jedah J.H. and Robinson R. K., Nutritional value and microbial safety of fresh fruit juices sold through retail outlets in Qatar, Pakistan J. Nutr.,,79-81 (2001)
  4. Mahale D.P, Khade R.G. and Vaidya V.K., Microbiological analysis of street vended fruit juices from Mumbai city, India, Internet J. Food Safety, 10,31-34 (2008)
  5. Kumari V., Nutritional and microbial quality of sugarcane juice in Udipur city. M.Sc dissertation, RAU, Bikaner., (1995)
  6. Andres S.C, Giannuzzi L. and Zaritzky N.E., The effect o temperature on microbial growth in apple cubes packed in film and preserved by use of orange juice, International J. Food Sci. and Tech.,39(9)927-933 (2004)
  7. Eckert J.W., Fungicidal and fungistatic agents: Control of pathogenic microorganisms on fresh fruits and vegetables after harvest, Food Mycology, Hall, Boston, 149-164 (1979)
  8. Chumber S.K., Kaushik K. and Savy S., Bacteriological analysis of street foods in Pune, Indian J. Public Health, 51 (2), 114-116 (2007)
  9. Ghosh M., Wahi S. and Kumar M. Ganguli., Prevalence of enterotoxigenic Staphylococcus aureus and Shigella spp. in some raw street vended Indian foods, Int. J. Environ. Health Res.,17(2), 151-6 (2007)
  10. Lewis J. E., Thompson P., Rao, B.V.V.B.N ., Kalavati, C. and Rjanna B., Human Bacteria in street vended Fruit Juices: A case study of Visakhapatnam City, India, Internet J. Food Safety, 35- 38 (2006)
  11. Muinde O.K. and Kuria E., Hygienic and sanitary practices of vendors of street foods in Nairobi, Kenya, AJFAND online www.ajfand.net 5 (1), 1-13 (2005)
  12. Mosupye F.M. and Van Holy A., Microbiological hazard identification and exposure assessment of street food vending in Johannesburg, South Africa, Int. J. Food Microbiol., 61,137-145 (2000)
  13. Beuchat L. R., Ecological factors influencing survival and growth of human pathogens on raw fruits and vegetables, Microbes Infect., 413-423 (2002)
  14. Hatcher W. S., Weihe J.L., Splittstoesser D.F., Hill E.C. and Parish M.E., Fruit beverages. In: Compendium of methods for the microbial examination of food. Vanderzant C and Splittstoesser D.F. (eds) American Public Health Association, Washigton DC., (1992)
  15. Burnett S.L. and Beuchal L.R., Human pathogens associated with raw produce and unpasteurized juices and difficulties in contamination, J. Indust. Microbiol. Biotechno.,27, 104-110 (2001)
  16. Mosupye F.M. and Holy Von A., Microbiological quality and safety of readt to eat street vended foods in Johannesburg South Africa,J. Food Protectio.,1278-1284(1999)
  17. Mukhopadhyay M., Majumdar M. and Basu P., Microbial contamination of street vended fruit juices in Kolkata city, India, Internet J. Food Safety., 13, 1-5 (2011)
  18. Titarmare A., Dabholka P. and Godbole S., Bacteiological analysis of street vended fresh fruit and vegetable juices in Nagpur city, India, Internet J. Food Safety, 11, 1-3 (2009)
  19. Tsige Ketema, Tsegaye Gaddisa and Ketema Bacha., Microbiological safety of fruit juices served in cafes/restaurants, Jimma town, south west Ethiopia, Ethopian J. Health Sci., 18(3), 95-100 (2008)
  20. Lateef A., Oloke J. K., Kana E.B. and Pacheco E., The microbiological quality of ice used to cool drinks and foods in Ogbomoso Metropolis, Southwest, Nigeria, Internet J. Food Safety, 39-43 (2006)
  21. Tambekar D.H., Jaiswal V.J., Dhanorkar D.V., Gulhane P.B. and Dudhane M.N., Microbial quality and safety of street vended fruit juices: A case study of Amravati city, Internet J. Food Safety, 10, 72-76 (2009)
  22. Bhaskar J., Usman M., Smitha S. and Bhat G.K., Bacteriological profile of street foods in Mangalore, Indian J. Med. Microbiol., 22,197-197 (2004)